Strain Fitness in Pseudomonas putida KT2440 around PP_3978

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,485,157	+		PP_3977	0.25	-1.0
4,485,157	+		PP_3977	0.25	+0.1
4,485,158	-				+1.2
4,485,189	+		PP_3977	0.28	-0.1
4,485,189	+		PP_3977	0.28	+1.2
4,485,190	-		PP_3977	0.28	-0.3
4,485,190	-		PP_3977	0.28	-0.2
4,485,190	-		PP_3977	0.28	+1.2
4,485,199	+		PP_3977	0.29	+0.2
4,485,199	+		PP_3977	0.29	+0.5
4,485,200	-		PP_3977	0.29	+0.2
4,485,207	+		PP_3977	0.29	+0.5
4,485,208	-		PP_3977	0.29	+0.1
4,485,215	+		PP_3977	0.30	-1.3
4,485,216	-		PP_3977	0.30	+0.1
4,485,216	-		PP_3977	0.30	-0.0
4,485,216	-		PP_3977	0.30	-0.6
4,485,216	-		PP_3977	0.30	-1.3
4,485,237	+		PP_3977	0.32	-0.4
4,485,238	-		PP_3977	0.32	+0.7
4,485,277	-		PP_3977	0.35	-0.5
4,485,277	-		PP_3977	0.35	-0.5
4,485,277	-		PP_3977	0.35	+0.5
4,485,310	-		PP_3977	0.37	-0.1
4,485,318	+		PP_3977	0.38	+1.2
4,485,318	+		PP_3977	0.38	-1.3
4,485,318	+		PP_3977	0.38	+1.1
4,485,407	+		PP_3977	0.45	+0.2
4,485,407	+		PP_3977	0.45	-0.7
4,485,408	-		PP_3977	0.45	-0.5
4,485,429	+		PP_3977	0.46	+0.2
4,485,429	+		PP_3977	0.46	-0.6
4,485,430	-		PP_3977	0.46	+1.6
4,485,430	-		PP_3977	0.46	-1.5
4,485,430	-		PP_3977	0.46	-0.2
4,485,456	-		PP_3977	0.48	-0.3
4,485,464	+		PP_3977	0.49	+0.9
4,485,464	+		PP_3977	0.49	+0.1
4,485,465	-		PP_3977	0.49	+0.2
4,485,496	+		PP_3977	0.52	-0.9
4,485,524	+		PP_3977	0.54	+1.2
4,485,525	-		PP_3977	0.54	+0.5
4,485,525	-		PP_3977	0.54	+0.2
4,485,590	+		PP_3977	0.59	-1.5
4,485,591	-		PP_3977	0.59	-0.5
4,485,630	-		PP_3977	0.62	-1.0
4,485,690	+		PP_3977	0.66	-0.1
4,485,690	+		PP_3977	0.66	-0.5
4,485,691	-		PP_3977	0.67	-0.9
4,485,768	+		PP_3977	0.72	-1.1
4,485,780	+		PP_3977	0.73	-1.7
4,485,780	+		PP_3977	0.73	-0.1
4,485,780	+		PP_3977	0.73	-0.2
4,485,780	+				+1.8
4,485,781	-		PP_3977	0.73	+0.4
4,485,781	-		PP_3977	0.73	+0.2
4,485,781	-		PP_3977	0.73	+0.7
4,485,799	-		PP_3977	0.75	-1.1
4,485,799	-		PP_3977	0.75	-0.1
4,485,903	+		PP_3977	0.83	-1.4
4,485,904	-		PP_3977	0.83	+0.1
4,485,962	+		PP_3977	0.87	-1.2
4,485,962	+		PP_3977	0.87	+1.8
4,485,963	-		PP_3977	0.87	+0.2
4,485,963	-		PP_3977	0.87	-0.2
4,485,970	+		PP_3977	0.88	-0.1
4,485,970	+		PP_3977	0.88	+0.2
4,485,970	+		PP_3977	0.88	-0.2
4,485,970	+		PP_3977	0.88	+0.3
4,485,970	+		PP_3977	0.88	-1.1
4,485,970	+		PP_3977	0.88	-0.4
4,485,970	+		PP_3977	0.88	-0.1
4,485,970	+		PP_3977	0.88	+0.7
4,485,970	+		PP_3977	0.88	-0.1
4,485,970	+		PP_3977	0.88	-0.6
4,485,971	-		PP_3977	0.88	-0.9
4,485,971	-		PP_3977	0.88	+2.8
4,485,971	-		PP_3977	0.88	+1.8
4,485,971	-		PP_3977	0.88	-1.0
4,485,971	-		PP_3977	0.88	+0.1
4,485,974	+		PP_3977	0.88	-2.2
4,485,975	-		PP_3977	0.88	+0.7
4,485,975	-		PP_3977	0.88	+0.8
4,485,975	-		PP_3977	0.88	+1.7
4,486,019	-				-0.6
4,486,028	+				+0.1
4,486,028	+				+1.2
4,486,033	+				-0.1
4,486,034	-				+0.2
4,486,034	-				+0.8
4,486,042	+				+0.4
4,486,092	-				-0.4
4,486,097	+				+0.6
4,486,097	+				+0.1
4,486,097	+				-0.4
4,486,097	+				+0.2
4,486,097	+				-0.7
4,486,097	+				-0.5
4,486,097	+				+0.9
4,486,097	+				+0.9
4,486,097	+				-0.3
4,486,097	+				-0.4
4,486,098	-				+1.1
4,486,098	-				+4.4
4,486,124	+				-0.3
4,486,124	+				-1.0
4,486,124	+				-0.9
4,486,124	+				-1.3
4,486,125	-				-1.5
4,486,125	-				+0.4
4,486,125	-				+1.0
4,486,128	+				+2.4
4,486,128	+				-0.0
4,486,128	+				+0.5
4,486,128	+				-0.1
4,486,128	+				+1.0
4,486,128	+				-0.1
4,486,128	+				-0.5
4,486,128	+				-2.6
4,486,128	+				-1.4
4,486,128	+				-0.6
4,486,128	+				-0.8
4,486,128	+				+0.4
4,486,128	+				-0.4
4,486,128	+				-0.5
4,486,128	+				-1.0
4,486,128	+				-0.7
4,486,129	-				+0.1
4,486,129	-				+0.2
4,486,129	-				+1.8
4,486,129	-				-1.3
4,486,129	-				+0.8
4,486,129	-				+1.6
4,486,129	-				+0.1
4,486,129	-				+0.4
4,486,129	-				+0.2
4,486,129	-				-0.6
4,486,129	-				+1.8
4,486,177	+		PP_3978	0.28	+0.3
4,486,177	+		PP_3978	0.28	-2.3
4,486,177	+		PP_3978	0.28	-1.8
4,486,177	+		PP_3978	0.28	+2.8
4,486,178	-		PP_3978	0.29	-0.3
4,486,178	-		PP_3978	0.29	-0.8
4,486,178	-		PP_3978	0.29	-0.9
4,486,178	-		PP_3978	0.29	-1.0
4,486,178	-		PP_3978	0.29	-1.7
4,486,260	+		PP_3978	0.77	-1.1
4,486,288	+				-0.8
4,486,288	+				-0.3
4,486,288	+				-0.3
4,486,335	-				-0.1