Strain Fitness in Pseudomonas putida KT2440 around PP_3708

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,229,482	-		PP_3707	0.49	+0.2
4,229,511	-		PP_3707	0.51	+0.8
4,229,940	+		PP_3707	0.81	+1.0
4,230,026	-		PP_3707	0.87	+0.1
4,230,028	-		PP_3707	0.87	-0.3
4,230,174	-				+0.8
4,230,186	+				+1.4
4,230,186	+				+0.2
4,230,186	+				-0.6
4,230,252	+				-0.3
4,230,252	+				-1.0
4,230,253	-				+1.8
4,230,253	-				+2.8
4,230,257	-				+0.5
4,230,257	-				+0.0
4,230,257	-				-1.6
4,230,265	+				-1.0
4,230,266	-				-1.7
4,230,379	-				+0.5
4,230,379	-		PP_3708	0.15	+0.8
4,230,379	-		PP_3708	0.15	-0.6
4,230,379	-		PP_3708	0.15	+0.5
4,230,383	-		PP_3708	0.15	+0.3
4,230,383	-		PP_3708	0.15	+0.2
4,230,415	-		PP_3708	0.18	+0.9
4,230,445	+		PP_3708	0.21	+0.7
4,230,446	-		PP_3708	0.21	+1.6
4,230,446	-		PP_3708	0.21	-0.0
4,230,446	-		PP_3708	0.21	+0.6
4,230,477	+		PP_3708	0.24	+0.1
4,230,477	+		PP_3708	0.24	+1.8
4,230,478	-		PP_3708	0.24	-0.5
4,230,478	-		PP_3708	0.24	+0.8
4,230,478	-		PP_3708	0.24	-1.3
4,230,500	-		PP_3708	0.26	+0.7
4,230,510	+		PP_3708	0.27	+0.1
4,230,521	-		PP_3708	0.28	-0.3
4,230,585	+		PP_3708	0.34	+0.8
4,230,630	+		PP_3708	0.39	+0.8
4,230,631	-		PP_3708	0.39	-0.1
4,230,837	-		PP_3708	0.59	+0.5
4,230,837	-		PP_3708	0.59	-0.4
4,230,856	-		PP_3708	0.61	-2.0
4,230,929	+		PP_3708	0.68	+0.4
4,230,929	+		PP_3708	0.68	+0.9
4,230,929	+		PP_3708	0.68	+0.6
4,230,930	-		PP_3708	0.68	-0.8
4,230,933	+		PP_3708	0.68	+0.2
4,230,933	+		PP_3708	0.68	+3.3
4,230,938	+		PP_3708	0.68	-0.3
4,230,969	-		PP_3708	0.71	+0.1
4,231,038	+		PP_3708	0.78	+0.0
4,231,038	+		PP_3708	0.78	+0.7
4,231,118	+		PP_3708	0.86	+0.2
4,231,123	-		PP_3708	0.86	-0.4
4,231,123	-		PP_3708	0.86	+0.2
4,231,201	-				+0.8
4,231,201	-				-0.1
4,231,230	-				+0.3
4,231,372	+				+0.5
4,231,372	+				-1.1
4,231,373	-				-0.3
4,231,373	-				+0.7
4,231,373	-				+1.8
4,231,382	+				-0.6
4,231,425	+		PP_3709	0.12	+0.7
4,231,425	+		PP_3709	0.12	+0.6
4,231,425	+		PP_3709	0.12	+0.2
4,231,425	+		PP_3709	0.12	+0.8
4,231,425	+		PP_3709	0.12	+0.6
4,231,426	-		PP_3709	0.12	+0.1
4,231,461	-		PP_3709	0.22	+1.3
4,231,477	-		PP_3709	0.27	-0.6
4,231,623	+		PP_3709	0.67	-0.3
4,231,624	-		PP_3709	0.68	-0.1
4,231,690	+		PP_3709	0.86	+0.3
4,231,691	-		PP_3709	0.86	+1.7
4,231,699	+		PP_3709	0.88	+0.6
4,231,699	+		PP_3709	0.88	+0.2
4,231,700	-		PP_3709	0.89	+1.3
4,231,756	-				+0.5
4,231,856	+		PP_5625	0.11	+0.2
4,231,856	+		PP_5625	0.11	-0.9
4,231,938	+		PP_5625	0.39	+0.6
4,231,971	+		PP_5625	0.51	-0.9
4,231,972	-		PP_5625	0.51	-0.4
4,231,972	-		PP_5625	0.51	-0.2
4,232,116	-				+0.8
4,232,186	+				-0.6
4,232,207	-				+0.6
4,232,207	-				-0.9
4,232,241	+				-0.1
4,232,257	-				+0.4