Strain Fitness in Pseudomonas putida KT2440 around PP_3450

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,907,488	+				+0.8
3,907,813	+		PP_3449	0.29	-0.8
3,907,940	-		PP_3449	0.59	+1.8
3,907,971	-		PP_3449	0.66	-0.4
3,908,093	-				+0.1
3,908,109	+				+0.6
3,908,110	-				+0.2
3,908,110	-				+0.4
3,908,322	+		PP_3450	0.10	+0.6
3,908,322	+		PP_3450	0.10	+0.2
3,908,322	+		PP_3450	0.10	+0.5
3,908,322	+		PP_3450	0.10	-0.6
3,908,322	+		PP_3450	0.10	-0.5
3,908,323	-		PP_3450	0.10	-0.2
3,908,323	-		PP_3450	0.10	-0.7
3,908,323	-		PP_3450	0.10	-0.7
3,908,676	+		PP_3450	0.24	+1.0
3,908,701	-		PP_3450	0.25	-1.0
3,908,842	+		PP_3450	0.30	-0.1
3,908,842	+		PP_3450	0.30	+0.3
3,908,842	+		PP_3450	0.30	+0.3
3,908,843	-		PP_3450	0.30	-1.5
3,908,843	-		PP_3450	0.30	-0.1
3,908,856	+		PP_3450	0.30	-0.3
3,908,856	+		PP_3450	0.30	+0.8
3,908,894	-		PP_3450	0.32	-0.2
3,909,129	+		PP_3450	0.41	-0.5
3,909,129	+		PP_3450	0.41	-0.7
3,909,130	-		PP_3450	0.41	-1.4
3,909,130	-		PP_3450	0.41	-1.8
3,909,130	-		PP_3450	0.41	+0.4
3,909,132	+		PP_3450	0.41	-0.9
3,909,132	+		PP_3450	0.41	-0.3
3,909,184	-		PP_3450	0.43	-0.4
3,909,459	+		PP_3450	0.53	+0.2
3,909,460	-		PP_3450	0.53	+0.5
3,909,460	-		PP_3450	0.53	+1.3
3,909,463	-		PP_3450	0.53	-0.4
3,909,752	-		PP_3450	0.64	-0.5
3,909,784	-		PP_3450	0.66	+1.1
3,909,854	+		PP_3450	0.68	+0.4
3,909,854	+		PP_3450	0.68	+0.5
3,909,855	-		PP_3450	0.68	-0.5
3,910,026	+		PP_3450	0.75	+1.8
3,910,026	+		PP_3450	0.75	-1.6
3,910,026	+		PP_3450	0.75	-1.0
3,910,026	+		PP_3450	0.75	-2.0
3,910,027	-		PP_3450	0.75	+2.1
3,910,027	-		PP_3450	0.75	+0.5
3,910,065	+		PP_3450	0.76	+0.4
3,910,134	+		PP_3450	0.79	+0.4
3,910,146	+		PP_3450	0.79	-0.1
3,910,201	+		PP_3450	0.81	-0.4
3,910,363	-		PP_3450	0.88	+0.0
3,910,363	-		PP_3450	0.88	-1.8
3,910,363	-		PP_3450	0.88	+0.1
3,910,696	+				-0.1
3,910,760	+				-0.3
3,910,760	+				+0.4
3,910,761	-				-1.0
3,910,761	-				+1.2
3,910,761	-				+0.2
3,910,961	+		PP_3451	0.32	+0.3
3,910,961	+		PP_3451	0.32	-0.6
3,910,961	+		PP_3451	0.32	+0.5
3,910,962	-				+2.2
3,910,976	+		PP_3451	0.34	+0.6
3,910,976	+		PP_3451	0.34	-0.7
3,910,976	+				+2.8
3,910,979	+		PP_3451	0.34	-0.4
3,911,022	-		PP_3451	0.39	-1.4
3,911,022	-		PP_3451	0.39	-1.1
3,911,102	+		PP_3451	0.49	-1.5
3,911,163	-		PP_3451	0.56	+1.2
3,911,209	+		PP_3451	0.62	-1.5
3,911,210	-		PP_3451	0.62	+0.9
3,911,210	-		PP_3451	0.62	-0.3
3,911,512	+				+2.2
3,911,581	+				+2.6
3,911,582	-				-0.2
3,911,582	-				+0.7
3,911,592	+				+0.6
3,911,592	+				-0.6
3,911,592	+				-1.3
3,911,593	-				+0.3
3,911,634	-				-0.6
3,911,680	+				-2.0
3,911,681	-				+0.5
3,911,681	-				-0.6
3,911,681	-				-1.6
3,911,681	-				-0.2