Strain Fitness in Pseudomonas putida KT2440 around PP_3292

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,725,830	+		PP_3291	0.35	-0.5
3,725,830	+		PP_3291	0.35	-0.3
3,725,831	-		PP_3291	0.35	+0.4
3,725,831	-		PP_3291	0.35	-0.6
3,725,968	+				+2.8
3,725,968	+		PP_3291	0.45	-0.3
3,725,968	+		PP_3291	0.45	+0.2
3,725,972	+		PP_3291	0.46	-1.4
3,725,972	+		PP_3291	0.46	+1.4
3,725,973	-		PP_3291	0.46	+0.1
3,726,012	-		PP_3291	0.49	-1.0
3,726,063	+		PP_3291	0.52	+0.5
3,726,101	+		PP_3291	0.55	+0.8
3,726,101	+		PP_3291	0.55	+2.8
3,726,101	+		PP_3291	0.55	+1.5
3,726,101	+		PP_3291	0.55	+0.2
3,726,101	+		PP_3291	0.55	+0.6
3,726,101	+		PP_3291	0.55	+2.8
3,726,102	-		PP_3291	0.55	+0.6
3,726,102	-		PP_3291	0.55	-0.6
3,726,102	-		PP_3291	0.55	-0.5
3,726,104	+		PP_3291	0.55	-0.1
3,726,104	+		PP_3291	0.55	-1.2
3,726,104	+		PP_3291	0.55	+0.8
3,726,104	+		PP_3291	0.55	-0.2
3,726,104	+		PP_3291	0.55	-0.3
3,726,104	+		PP_3291	0.55	-0.3
3,726,104	+		PP_3291	0.55	+0.5
3,726,104	+		PP_3291	0.55	+1.5
3,726,104	+		PP_3291	0.55	+0.5
3,726,104	+		PP_3291	0.55	+0.3
3,726,105	-		PP_3291	0.55	+1.3
3,726,105	-		PP_3291	0.55	+0.4
3,726,105	-		PP_3291	0.55	+0.2
3,726,110	+		PP_3291	0.56	-0.4
3,726,111	-		PP_3291	0.56	-0.2
3,726,111	-		PP_3291	0.56	+1.1
3,726,111	-		PP_3291	0.56	-0.8
3,726,111	-		PP_3291	0.56	-0.6
3,726,152	+		PP_3291	0.59	-1.9
3,726,153	-		PP_3291	0.59	+0.0
3,726,153	-		PP_3291	0.59	+0.0
3,726,490	+		PP_3291	0.83	-0.0
3,726,491	-		PP_3291	0.84	-0.2
3,726,593	+				+0.5
3,726,593	+				+0.8
3,726,594	-				-1.4
3,726,594	-				-0.5
3,726,594	-				-0.5
3,726,594	-				+0.3
3,726,784	-				+0.7
3,726,784	-				-0.7
3,726,803	-				+0.2
3,726,858	+		PP_3292	0.11	-0.7
3,726,858	+		PP_3292	0.11	+0.3
3,726,859	-		PP_3292	0.11	-1.1
3,726,874	-		PP_3292	0.13	-0.7
3,727,214	-		PP_3292	0.66	-0.9
3,727,214	-		PP_3292	0.66	-2.1
3,727,215	+		PP_3292	0.66	-1.1
3,727,215	+		PP_3292	0.66	+0.6
3,727,215	+		PP_3292	0.66	-1.7
3,727,216	-				+0.2
3,727,216	-		PP_3292	0.66	-1.3
3,727,493	+				-0.1
3,727,589	+		PP_3293	0.23	+0.7
3,727,635	+		PP_3293	0.34	+1.5
3,727,636	-		PP_3293	0.34	+0.1
3,727,637	+		PP_3293	0.34	-2.8
3,727,637	+		PP_3293	0.34	-0.3
3,727,638	-		PP_3293	0.34	+0.1
3,727,638	-		PP_3293	0.34	-0.6
3,727,638	-		PP_3293	0.34	+1.0
3,727,638	-		PP_3293	0.34	+0.0
3,727,638	-		PP_3293	0.34	-1.9
3,727,638	-		PP_3293	0.34	+0.6
3,727,638	-		PP_3293	0.34	-0.4
3,727,727	+		PP_3293	0.55	+1.5
3,727,727	+		PP_3293	0.55	+0.2
3,727,728	-		PP_3293	0.55	+0.7
3,727,728	-		PP_3293	0.55	+0.2
3,727,730	+		PP_3293	0.56	+1.1
3,727,730	+		PP_3293	0.56	-1.1
3,727,730	+		PP_3293	0.56	+0.0
3,727,859	+		PP_3293	0.86	+1.8
3,727,978	+				+0.1
3,727,978	+				+0.8
3,727,979	-				+0.1
3,728,006	-				+0.0
3,728,019	+				+0.2
3,728,019	+				-0.8
3,728,019	+				+0.2
3,728,019	+				-0.1
3,728,019	+				+0.3
3,728,020	-				+0.3
3,728,053	+				-1.0
3,728,053	+				-0.3
3,728,054	-				-0.5
3,728,054	-				+0.8
3,728,057	+				+1.0
3,728,057	+				-0.9
3,728,058	-				-0.9
3,728,087	-				+0.7
3,728,125	+				+0.3
3,728,125	+				-0.4
3,728,126	-				+0.1
3,728,157	-				+1.4
3,728,236	+		PP_3294	0.12	-0.1
3,728,236	+		PP_3294	0.12	-1.7
3,728,237	-		PP_3294	0.12	-0.7
3,728,260	+		PP_3294	0.15	-2.6