Strain Fitness in Pseudomonas putida KT2440 around PP_3039

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,423,121	+		PP_3038	0.58	-1.9
3,423,122	-		PP_3038	0.58	-0.4
3,423,202	+		PP_3038	0.61	+0.7
3,423,203	-		PP_3038	0.62	+0.9
3,423,203	-		PP_3038	0.62	+0.1
3,423,267	+		PP_3038	0.64	+0.3
3,423,268	-		PP_3038	0.64	-0.7
3,423,358	-		PP_3038	0.69	+2.8
3,423,358	-		PP_3038	0.69	-0.2
3,423,405	+		PP_3038	0.71	-1.0
3,423,405	+		PP_3038	0.71	-0.3
3,423,406	-		PP_3038	0.71	-0.8
3,423,486	+		PP_3038	0.74	-0.5
3,423,486	+		PP_3038	0.74	-0.2
3,423,486	+		PP_3038	0.74	-0.5
3,423,487	-		PP_3038	0.74	+1.1
3,423,496	+		PP_3038	0.75	-0.6
3,423,509	-		PP_3038	0.75	-0.2
3,423,574	+		PP_3038	0.78	+0.2
3,423,575	-		PP_3038	0.78	-0.6
3,423,575	-		PP_3038	0.78	+0.1
3,423,575	-		PP_3038	0.78	-0.4
3,423,575	-		PP_3038	0.78	-0.3
3,423,579	+		PP_3038	0.79	+0.1
3,423,579	+		PP_3038	0.79	+0.5
3,423,605	-		PP_3038	0.80	-0.8
3,423,767	+		PP_3038	0.87	-1.1
3,423,767	+		PP_3038	0.87	+0.8
3,423,966	+				-1.5
3,423,967	-				+0.2
3,423,982	+				+0.2
3,424,063	+				+0.1
3,424,063	+				-0.7
3,424,063	+				-0.2
3,424,063	+				-1.1
3,424,063	+				+1.2
3,424,063	+				-0.1
3,424,063	+				-0.4
3,424,064	-				+0.5
3,424,064	-				-0.8
3,424,064	-				-0.8
3,424,137	-		PP_3039	0.24	+1.0
3,424,137	-		PP_3039	0.24	-0.8
3,424,137	-		PP_3039	0.24	+0.5
3,424,205	-		PP_3039	0.43	+0.1
3,424,205	-		PP_3039	0.43	-0.4
3,424,205	-		PP_3039	0.43	+0.4
3,424,205	-		PP_3039	0.43	+0.4
3,424,205	-		PP_3039	0.43	+0.6
3,424,241	+		PP_3039	0.53	+0.4
3,424,241	+		PP_3039	0.53	+1.0
3,424,242	-		PP_3039	0.53	+0.3
3,424,284	+		PP_3039	0.64	-0.5
3,424,285	-		PP_3039	0.65	+0.2
3,424,285	-		PP_3039	0.65	+1.7
3,424,318	-		PP_3039	0.74	+0.8
3,424,334	+				-0.9
3,424,343	+		PP_3039	0.81	-0.9
3,424,344	-		PP_3039	0.81	-0.4
3,424,344	-		PP_3039	0.81	-1.8
3,424,347	+		PP_3039	0.82	-0.8
3,424,347	+		PP_3039	0.82	-0.0
3,424,347	+		PP_3039	0.82	+0.3
3,424,347	+		PP_3039	0.82	+1.0
3,424,348	-		PP_3039	0.82	+0.5
3,424,350	+		PP_3039	0.83	+0.9
3,424,350	+		PP_3039	0.83	-0.1
3,424,350	+		PP_3039	0.83	+0.3
3,424,351	-		PP_3039	0.83	-0.6
3,424,351	-		PP_3039	0.83	-0.9
3,424,360	+		PP_3039	0.85	+1.2
3,424,361	-		PP_3039	0.86	+1.2
3,424,379	+				-0.8
3,424,431	-				+0.0
3,424,474	-				-1.2
3,424,518	+				-0.6
3,424,538	+				-0.1
3,424,539	-				+0.4
3,424,539	-				+0.3
3,424,622	+				+0.6
3,424,647	+				+0.5
3,424,691	+				+0.5
3,424,692	-				-0.9
3,424,715	+				-0.5
3,424,716	-				-1.2
3,424,716	-				-0.3
3,424,730	+				-2.0
3,424,731	-				-0.7
3,424,731	-				+1.8
3,424,767	+				-0.8
3,424,774	+				+0.5
3,424,775	-				-0.7
3,424,790	+				+0.1
3,424,791	-				-0.1
3,424,823	+				+0.7
3,424,946	+				+1.8
3,424,946	+				-0.7
3,424,946	+				-0.7
3,424,946	+				-0.1
3,424,947	-				+0.5
3,425,193	-		PP_3040	0.77	-0.6
3,425,200	+		PP_3040	0.79	-1.2
3,425,201	-		PP_3040	0.80	+0.3
3,425,201	-				+1.2
3,425,371	+				+0.7