Strain Fitness in Pseudomonas putida KT2440 around PP_2833

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,232,810	+				-1.0
3,232,812	+				-1.4
3,232,976	+		PP_2832	0.17	+0.1
3,232,977	-		PP_2832	0.17	+0.1
3,233,003	+		PP_2832	0.20	-1.6
3,233,232	+		PP_2832	0.44	-0.6
3,233,232	+		PP_2832	0.44	+0.8
3,233,232	+		PP_2832	0.44	-0.9
3,233,232	+		PP_2832	0.44	-0.4
3,233,232	+		PP_2832	0.44	+1.3
3,233,233	-		PP_2832	0.44	-0.6
3,233,291	+		PP_2832	0.50	-0.6
3,233,296	-		PP_2832	0.51	+1.0
3,233,344	-		PP_2832	0.56	+1.1
3,233,378	+		PP_2832	0.60	+0.7
3,233,379	-		PP_2832	0.60	+1.0
3,233,379	-		PP_2832	0.60	+1.9
3,233,623	+		PP_2832	0.85	-3.0
3,233,624	-		PP_2832	0.86	+1.0
3,233,624	-		PP_2832	0.86	-1.0
3,233,731	-				+0.8
3,233,744	-				-1.8
3,233,744	-				-0.4
3,233,758	+				+1.1
3,233,862	-				-0.5
3,234,013	+		PP_2833	0.23	-1.4
3,234,013	+		PP_2833	0.23	-0.9
3,234,014	-		PP_2833	0.23	+0.1
3,234,014	-		PP_2833	0.23	-0.4
3,234,140	+		PP_2833	0.36	-0.7
3,234,140	+		PP_2833	0.36	-1.0
3,234,141	-		PP_2833	0.36	-0.9
3,234,183	-		PP_2833	0.41	-2.2
3,234,183	-		PP_2833	0.41	-1.0
3,234,183	-		PP_2833	0.41	-2.2
3,234,369	+		PP_2833	0.60	-0.9
3,234,423	+		PP_2833	0.65	+1.3
3,234,637	+		PP_2833	0.87	-1.2
3,234,637	+		PP_2833	0.87	-0.4
3,234,784	+				-2.6
3,234,853	+				-1.7
3,234,854	-				-0.1
3,234,854	-				-2.3
3,234,854	-				+1.9
3,234,854	-				-0.1
3,234,863	-				+0.3
3,234,871	-				-1.9
3,235,192	-		PP_2834	0.19	+1.0
3,235,284	+		PP_2834	0.25	+0.6
3,235,285	-		PP_2834	0.25	+2.9
3,235,285	-		PP_2834	0.25	+0.3
3,235,604	+		PP_2834	0.45	-0.3
3,235,605	-		PP_2834	0.45	+0.6