Strain Fitness in Pseudomonas putida KT2440 around PP_2748

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,130,191	+				+0.3
3,130,191	+				-0.6
3,130,192	-				+0.1
3,130,192	-				-0.1
3,130,192	-				-0.7
3,130,192	-				+0.3
3,130,221	+				-1.5
3,130,570	-		PP_2747	0.40	-1.0
3,130,807	-				+0.3
3,130,825	-		PP_2747	0.66	-0.1
3,131,080	+				-0.3
3,131,081	-				+0.4
3,131,081	-				+0.1
3,131,081	-				-0.5
3,131,081	-				-1.3
3,131,101	-				-1.2
3,131,135	+				+1.5
3,131,312	-		PP_2748	0.24	-0.5
3,131,615	-		PP_2748	0.62	-1.4
3,131,841	+		PP_2748	0.90	-0.5
3,131,841	+		PP_2748	0.90	-1.0
3,132,003	+				-0.6
3,132,003	+				-0.7
3,132,122	-		PP_2749	0.23	-1.6
3,132,269	-		PP_2749	0.40	-0.2
3,132,574	+		PP_2749	0.74	-0.3
3,132,574	+		PP_2749	0.74	-0.2
3,132,682	-		PP_2749	0.87	-1.4
3,132,751	-				+1.3
3,132,751	-				-0.6
3,132,751	-				+0.1
3,132,751	-				-0.9
3,132,797	-				-0.3