Strain Fitness in Pseudomonas putida KT2440 around PP_2736

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,118,737	-		PP_2735	0.13	+0.6
3,118,758	+		PP_2735	0.16	+0.7
3,118,758	+		PP_2735	0.16	+1.0
3,118,759	-		PP_2735	0.16	-0.2
3,118,806	+		PP_2735	0.22	-1.1
3,118,806	+		PP_2735	0.22	+2.2
3,118,807	-		PP_2735	0.22	+0.6
3,118,807	-		PP_2735	0.22	+0.0
3,118,904	+		PP_2735	0.34	-1.4
3,118,904	+		PP_2735	0.34	-1.1
3,118,904	+		PP_2735	0.34	+0.3
3,118,904	+		PP_2735	0.34	+1.3
3,118,904	+		PP_2735	0.34	-0.1
3,119,024	+		PP_2735	0.49	+2.9
3,119,025	-				+0.7
3,119,025	-		PP_2735	0.49	-0.6
3,119,029	-		PP_2735	0.49	+0.6
3,119,029	-		PP_2735	0.49	+0.1
3,119,352	+		PP_2735	0.89	+2.1
3,119,353	-		PP_2735	0.89	+0.4
3,119,529	-				+0.2
3,119,592	-		PP_2736	0.12	+0.8
3,119,592	-		PP_2736	0.12	-0.1
3,119,638	+		PP_2736	0.16	+0.6
3,119,638	+		PP_2736	0.16	-0.6
3,119,639	-		PP_2736	0.16	-1.2
3,119,688	+		PP_2736	0.20	+1.0
3,119,832	-		PP_2736	0.32	-0.4
3,120,045	-		PP_2736	0.49	+1.0
3,120,194	-		PP_2736	0.61	+0.0
3,120,194	-		PP_2736	0.61	+0.8
3,120,200	+		PP_2736	0.61	+1.5
3,120,201	-		PP_2736	0.61	-1.1
3,120,201	-		PP_2736	0.61	-0.1
3,120,201	-		PP_2736	0.61	+1.9
3,120,201	-		PP_2736	0.61	+0.1
3,120,396	+		PP_2736	0.77	+0.5
3,120,398	+		PP_2736	0.77	+1.0
3,120,398	+		PP_2736	0.77	+0.1
3,120,494	+		PP_2736	0.85	+1.9
3,120,495	-		PP_2736	0.85	-0.9
3,120,526	-		PP_2736	0.87	+0.7
3,120,529	+		PP_2736	0.88	-0.9
3,120,530	-		PP_2736	0.88	+0.7
3,120,530	-		PP_2736	0.88	+0.3
3,120,530	-		PP_2736	0.88	-1.3
3,120,530	-		PP_2736	0.88	+1.3
3,120,677	-				-0.4
3,120,678	+				+2.1
3,120,679	-				+1.2
3,120,679	-				+0.0
3,120,831	+		PP_2737	0.19	+0.8
3,121,182	-		PP_2737	0.65	+0.1
3,121,407	+				-1.0
3,121,498	+		PP_2738	0.14	+0.8
3,121,637	-		PP_2738	0.47	-1.8
3,121,678	+		PP_2738	0.56	-0.6