Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2723 and PP_2724 are separated by 194 nucleotides PP_2724 and PP_2725 are separated by 56 nucleotides PP_2725 and PP_2726 are separated by 89 nucleotides PP_2726 and PP_2727 are separated by 95 nucleotides
PP_2723: PP_2723 - Uncharacterized oxidoreductase YhdF, at 3,109,323 to 3,110,180
_2723
PP_2724: PP_2724 - conserved protein of unknown function, at 3,110,375 to 3,110,899
_2724
PP_2725: PP_2725 - Protease PfpI, at 3,110,956 to 3,111,498
_2725
PP_2726: PP_2726 - conserved membrane protein of unknown function, at 3,111,588 to 3,112,280
_2726
PP_2727: PP_2727 - putative C-factor, at 3,112,376 to 3,113,146
_2727
Position (kb)
3110
3111
3112 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3109.964 kb on - strand, within PP_2723 at 3109.996 kb on + strand, within PP_2723 at 3109.997 kb on - strand, within PP_2723 at 3110.081 kb on - strand, within PP_2723 at 3110.166 kb on - strand at 3110.166 kb on - strand at 3110.313 kb on + strand at 3110.487 kb on - strand, within PP_2724 at 3110.555 kb on + strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.556 kb on - strand, within PP_2724 at 3110.690 kb on - strand, within PP_2724 at 3110.753 kb on + strand, within PP_2724 at 3110.753 kb on + strand, within PP_2724 at 3111.063 kb on + strand, within PP_2725 at 3111.102 kb on - strand, within PP_2725 at 3111.102 kb on - strand, within PP_2725 at 3111.355 kb on + strand, within PP_2725 at 3111.516 kb on - strand at 3111.526 kb on + strand at 3111.526 kb on + strand at 3111.574 kb on - strand at 3111.629 kb on + strand at 3111.629 kb on + strand at 3111.629 kb on + strand at 3111.630 kb on - strand at 3111.630 kb on - strand at 3111.790 kb on + strand, within PP_2726 at 3111.797 kb on - strand, within PP_2726 at 3111.871 kb on - strand, within PP_2726 at 3111.922 kb on - strand, within PP_2726 at 3111.928 kb on + strand, within PP_2726 at 3111.929 kb on - strand, within PP_2726 at 3112.065 kb on + strand, within PP_2726 at 3112.232 kb on + strand at 3112.248 kb on + strand at 3112.248 kb on + strand at 3112.249 kb on - strand at 3112.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 3,109,964 - PP_2723 0.75 +0.4 3,109,996 + PP_2723 0.78 -0.5 3,109,997 - PP_2723 0.79 -0.7 3,110,081 - PP_2723 0.88 +0.8 3,110,166 - -1.2 3,110,166 - +0.3 3,110,313 + -0.1 3,110,487 - PP_2724 0.21 +2.9 3,110,555 + PP_2724 0.34 +0.5 3,110,556 - PP_2724 0.34 -0.2 3,110,556 - PP_2724 0.34 +0.3 3,110,556 - PP_2724 0.34 -0.3 3,110,690 - PP_2724 0.60 -1.4 3,110,753 + PP_2724 0.72 -0.3 3,110,753 + PP_2724 0.72 -0.2 3,111,063 + PP_2725 0.20 -1.7 3,111,102 - PP_2725 0.27 -2.3 3,111,102 - PP_2725 0.27 -0.8 3,111,355 + PP_2725 0.73 +0.5 3,111,516 - -2.2 3,111,526 + -0.1 3,111,526 + +0.1 3,111,574 - +0.1 3,111,629 + -0.5 3,111,629 + +0.5 3,111,629 + -0.3 3,111,630 - -0.0 3,111,630 - -0.4 3,111,790 + PP_2726 0.29 -0.7 3,111,797 - PP_2726 0.30 -0.4 3,111,871 - PP_2726 0.41 +0.6 3,111,922 - PP_2726 0.48 +0.8 3,111,928 + PP_2726 0.49 +0.9 3,111,929 - PP_2726 0.49 -0.1 3,112,065 + PP_2726 0.69 +1.4 3,112,232 + +0.1 3,112,248 + -2.0 3,112,248 + -0.6 3,112,249 - +0.8 3,112,312 + +0.5
Or see this region's nucleotide sequence