Strain Fitness in Pseudomonas putida KT2440 around PP_2496

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,843,764	-		PP_2495	0.13	+0.1
2,843,883	+		PP_2495	0.25	+0.1
2,843,883	+		PP_2495	0.25	-0.9
2,844,013	-		PP_2495	0.38	+1.3
2,844,107	+		PP_2495	0.47	+1.0
2,844,108	-		PP_2495	0.47	-1.1
2,844,113	+		PP_2495	0.47	-0.0
2,844,113	+		PP_2495	0.47	+0.0
2,844,113	+		PP_2495	0.47	-1.1
2,844,114	-		PP_2495	0.48	-0.4
2,844,114	-		PP_2495	0.48	-0.8
2,844,114	-		PP_2495	0.48	+0.7
2,844,172	-		PP_2495	0.53	-0.4
2,844,172	-		PP_2495	0.53	+1.3
2,844,325	+		PP_2495	0.69	+1.4
2,844,326	-		PP_2495	0.69	-1.3
2,844,343	+		PP_2495	0.70	+0.6
2,844,343	+		PP_2495	0.70	+0.3
2,844,344	-		PP_2495	0.70	+2.3
2,844,344	-		PP_2495	0.70	-0.5
2,844,344	-		PP_2495	0.70	-0.7
2,844,344	-		PP_2495	0.70	+2.3
2,844,357	+		PP_2495	0.72	+0.0
2,844,357	+		PP_2495	0.72	-0.8
2,844,358	-		PP_2495	0.72	+1.4
2,844,358	-		PP_2495	0.72	-0.3
2,844,396	+		PP_2495	0.76	+0.3
2,844,396	+		PP_2495	0.76	+0.1
2,844,396	+		PP_2495	0.76	-0.5
2,844,396	+		PP_2495	0.76	+1.3
2,844,400	+		PP_2495	0.76	+0.3
2,844,400	+		PP_2495	0.76	-1.9
2,844,400	+		PP_2495	0.76	-1.0
2,844,401	-		PP_2495	0.76	-0.7
2,844,401	-		PP_2495	0.76	-0.4
2,844,401	-		PP_2495	0.76	+0.1
2,844,401	-		PP_2495	0.76	-0.5
2,844,401	-		PP_2495	0.76	-0.6
2,844,401	-		PP_2495	0.76	-0.4
2,844,401	-		PP_2495	0.76	+1.1
2,844,401	-		PP_2495	0.76	+0.5
2,844,484	+		PP_2495	0.84	-0.6
2,844,484	+		PP_2495	0.84	+0.6
2,844,484	+		PP_2495	0.84	-1.2
2,844,485	-		PP_2495	0.84	+0.1
2,844,485	-		PP_2495	0.84	-1.9
2,844,486	+		PP_2495	0.85	+0.2
2,844,640	+				-0.3
2,844,640	+				-1.9
2,844,640	+				+2.1
2,844,641	-				+0.1
2,844,641	-				-0.2
2,844,641	-				-1.1
2,844,714	-				+0.9
2,844,743	+				-1.0
2,845,064	-		PP_2496	0.33	-2.2
2,845,064	-		PP_2496	0.33	-0.2
2,845,268	-		PP_2496	0.53	-1.9
2,845,268	-		PP_2496	0.53	+1.2
2,845,268	-		PP_2496	0.53	-0.7
2,845,268	-		PP_2496	0.53	-1.1
2,845,578	-		PP_2496	0.83	-1.0
2,845,660	+				+1.0
2,845,661	-				-0.6
2,845,760	+				-1.7
2,845,760	+				-0.3
2,845,760	+				+0.3
2,845,760	+				+0.5
2,845,761	-				-1.1
2,845,761	-				+0.4
2,845,819	+		PP_2497	0.10	-0.5
2,845,819	+		PP_2497	0.10	-0.4
2,846,150	+				-1.1
2,846,151	-				-1.4
2,846,151	-				-0.2
2,846,151	-				-0.5
2,846,194	+				-1.8
2,846,195	-				+0.7
2,846,196	+				+1.0
2,846,214	+				-1.1
2,846,214	+				-0.4
2,846,215	-				+0.6
2,846,215	-				+0.7
2,846,220	+				-1.0
2,846,220	+				-0.3
2,846,412	-				+3.9
2,846,412	-		PP_2498	0.37	+0.7
2,846,437	-		PP_2498	0.48	+0.2
2,846,443	+		PP_2498	0.50	-0.0
2,846,443	+		PP_2498	0.50	-0.8
2,846,444	-		PP_2498	0.50	-0.0
2,846,444	-		PP_2498	0.50	-0.4
2,846,444	-		PP_2498	0.50	-1.1
2,846,464	+		PP_2498	0.59	+0.2
2,846,465	-		PP_2498	0.59	+0.3
2,846,465	-		PP_2498	0.59	+1.3
2,846,474	+		PP_2498	0.63	+0.3
2,846,475	-		PP_2498	0.63	-0.7
2,846,563	+				+0.1
2,846,563	+				+0.0
2,846,618	+				-0.7
2,846,618	+				-0.3
2,846,700	-				-1.3