Strain Fitness in Pseudomonas putida KT2440 around PP_2459

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,805,396	+		PP_2458	0.19	-0.0
2,805,396	+		PP_2458	0.19	+0.8
2,805,424	+				+1.3
2,805,425	-		PP_2458	0.22	-0.6
2,805,451	+		PP_2458	0.25	+0.8
2,805,451	+		PP_2458	0.25	-0.2
2,805,451	+		PP_2458	0.25	-0.2
2,805,451	+		PP_2458	0.25	-0.9
2,805,452	-		PP_2458	0.25	+0.5
2,805,452	-		PP_2458	0.25	-0.0
2,805,452	-		PP_2458	0.25	+0.3
2,805,452	-		PP_2458	0.25	-0.1
2,805,546	+		PP_2458	0.35	+0.2
2,805,546	+		PP_2458	0.35	+2.8
2,805,546	+		PP_2458	0.35	+0.8
2,805,546	+		PP_2458	0.35	-0.3
2,805,547	-		PP_2458	0.35	-0.0
2,805,547	-		PP_2458	0.35	-0.7
2,805,547	-		PP_2458	0.35	-0.5
2,805,629	+		PP_2458	0.44	+1.0
2,805,629	+		PP_2458	0.44	-1.2
2,805,630	-		PP_2458	0.44	+0.8
2,805,630	-		PP_2458	0.44	+1.3
2,805,630	-		PP_2458	0.44	+0.2
2,805,630	-		PP_2458	0.44	-0.3
2,805,919	-		PP_2458	0.76	-2.7
2,805,940	+		PP_2458	0.79	-0.5
2,805,987	+		PP_2458	0.84	+1.3
2,806,262	+		PP_2459	0.33	-0.8
2,806,472	+		PP_2459	0.85	-0.7
2,806,473	-		PP_2459	0.86	-1.8
2,806,491	+				+0.0
2,806,527	+				-2.0
2,806,528	-				+1.4
2,806,573	+				+0.5
2,806,574	-				+0.3
2,806,610	+				-0.5
2,806,611	-				-0.6
2,806,611	-				-0.9
2,806,847	-		PP_2460	0.23	-0.9
2,806,926	+				+0.5
2,806,926	-		PP_2460	0.31	-0.8
2,806,976	-		PP_2460	0.36	-0.0
2,806,976	-		PP_2460	0.36	-0.8
2,807,164	+		PP_2460	0.55	-0.2
2,807,218	+		PP_2460	0.61	-0.9
2,807,329	+		PP_2460	0.72	-0.5
2,807,329	+		PP_2460	0.72	-0.8
2,807,329	+		PP_2460	0.72	+0.9
2,807,329	+		PP_2460	0.72	-0.5
2,807,330	-		PP_2460	0.73	-0.9
2,807,330	-		PP_2460	0.73	+0.8
2,807,353	+		PP_2460	0.75	+0.1
2,807,353	+		PP_2460	0.75	+1.2
2,807,354	-		PP_2460	0.75	-0.5
2,807,354	-		PP_2460	0.75	-0.0
2,807,360	+		PP_2460	0.76	+0.2
2,807,360	+		PP_2460	0.76	-0.3
2,807,361	-		PP_2460	0.76	+0.2
2,807,399	-		PP_2460	0.80	+0.4
2,807,405	+		PP_2460	0.80	-0.7
2,807,405	+		PP_2460	0.80	-1.3