Strain Fitness in Pseudomonas putida KT2440 around PP_2417

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,761,925	-		PP_2415	0.80	-0.8
2,761,925	-		PP_2415	0.80	-0.4
2,761,932	+		PP_2415	0.81	-0.4
2,761,932	+		PP_2415	0.81	+0.4
2,761,932	+		PP_2415	0.81	-0.6
2,761,933	-		PP_2415	0.81	-0.1
2,761,933	-				+1.8
2,762,043	-				+0.7
2,762,158	+				+1.5
2,762,159	-				-0.5
2,762,298	+		PP_2416	0.24	-0.4
2,762,344	-		PP_2416	0.30	+0.2
2,762,344	-		PP_2416	0.30	-1.4
2,763,548	+		PP_2417	0.64	-2.5
2,763,548	+		PP_2417	0.64	+0.8
2,763,624	+		PP_2417	0.72	-0.0
2,763,727	-		PP_2417	0.82	-0.8
2,763,727	-		PP_2417	0.82	-1.0
2,764,002	+				-0.5
2,764,162	+		PP_2418	0.23	-0.9
2,764,254	-		PP_2418	0.32	-0.2
2,764,254	-		PP_2418	0.32	+0.4
2,764,254	-		PP_2418	0.32	-0.4
2,764,254	-		PP_2418	0.32	+0.1
2,764,427	+		PP_2418	0.47	+1.4
2,764,427	+		PP_2418	0.47	+1.6
2,764,428	-		PP_2418	0.48	-0.6
2,764,514	+		PP_2418	0.55	-1.4
2,764,515	-		PP_2418	0.56	+0.0
2,764,515	-		PP_2418	0.56	-0.2
2,764,515	-		PP_2418	0.56	+0.1
2,764,515	-		PP_2418	0.56	+1.3
2,764,515	-		PP_2418	0.56	+0.4
2,764,682	+		PP_2418	0.71	+1.8
2,764,682	+		PP_2418	0.71	-0.5
2,764,693	+		PP_2418	0.72	-0.2
2,764,694	-		PP_2418	0.72	+0.4
2,764,694	-		PP_2418	0.72	-0.2
2,764,748	+		PP_2418	0.77	-0.9
2,764,748	+		PP_2418	0.77	-1.0
2,764,748	+		PP_2418	0.77	+1.3
2,764,749	-		PP_2418	0.77	-0.3
2,764,882	+		PP_2418	0.89	+0.9
2,764,882	+		PP_2418	0.89	-0.2
2,764,883	-		PP_2418	0.89	+0.3
2,764,883	-		PP_2418	0.89	-0.6