Strain Fitness in Pseudomonas putida KT2440 around PP_2373

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,709,596	+		PP_2372	0.16	+0.6
2,709,596	+		PP_2372	0.16	-0.2
2,709,596	+		PP_2372	0.16	-2.2
2,709,596	+		PP_2372	0.16	-0.4
2,709,597	-		PP_2372	0.16	-0.2
2,709,597	-		PP_2372	0.16	+0.0
2,709,598	+		PP_2372	0.16	+0.6
2,709,598	+		PP_2372	0.16	-0.1
2,709,692	+		PP_2372	0.25	+0.4
2,709,692	+		PP_2372	0.25	+0.1
2,709,693	-		PP_2372	0.25	+1.7
2,709,693	-		PP_2372	0.25	+1.0
2,709,806	+		PP_2372	0.36	+1.8
2,709,807	-		PP_2372	0.36	-1.2
2,709,807	-		PP_2372	0.36	-0.4
2,709,807	-		PP_2372	0.36	-0.9
2,709,823	+		PP_2372	0.37	-0.3
2,709,823	+		PP_2372	0.37	+0.1
2,710,020	-		PP_2372	0.56	+1.1
2,710,046	+		PP_2372	0.58	-1.8
2,710,047	-		PP_2372	0.58	-0.8
2,710,239	+		PP_2372	0.76	-0.1
2,710,239	+		PP_2372	0.76	+0.4
2,710,240	-		PP_2372	0.76	-0.1
2,710,433	+				+0.6
2,710,533	+				-1.0
2,710,569	-		PP_2373	0.17	-0.8
2,710,677	+		PP_2373	0.87	-1.1
2,710,678	-		PP_2373	0.87	-0.4
2,710,721	-				+0.6
2,710,721	-				-0.1
2,710,721	-				+0.8
2,710,762	+				+0.1
2,710,796	+				+0.9
2,710,797	-				+0.2
2,710,825	+				-0.1
2,710,925	+				+0.0
2,710,975	+				+0.0
2,711,266	+				+1.5
2,711,266	+				-1.2
2,711,266	+				+0.3
2,711,267	-				+0.3
2,711,267	-				-0.0
2,711,267	-				+0.4
2,711,327	+				+0.3
2,711,327	+				-2.3
2,711,328	-				-0.0
2,711,355	+				-0.3
2,711,356	-				+0.2
2,711,398	-				-0.3
2,711,398	-				-0.0
2,711,417	-				+0.5
2,711,447	-				+0.5
2,711,470	-				-1.7
2,711,586	+				+0.5
2,711,586	+				+0.3
2,711,586	+				-1.1
2,711,587	-				+0.4