Strain Fitness in Pseudomonas putida KT2440 around PP_2150

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,456,758	+				-0.6
2,456,758	+				-0.4
2,456,758	+				-0.6
2,456,759	-				+0.2
2,456,854	+				+1.6
2,456,855	-				-1.3
2,456,863	-				-0.1
2,456,877	-				-0.5
2,456,877	-				+0.8
2,456,943	+				+2.8
2,456,961	+				+1.8
2,456,961	+				+0.3
2,457,027	-				+0.8
2,457,027	-				-1.9
2,457,027	-				+0.1
2,457,027	-				+1.2
2,457,039	-		PP_2150	0.11	-0.3
2,457,120	-		PP_2150	0.19	+1.0
2,457,563	-		PP_2150	0.63	+0.5
2,457,622	+		PP_2150	0.69	+0.6
2,457,633	+		PP_2150	0.70	+0.3
2,457,659	+		PP_2150	0.73	+1.4
2,457,660	-		PP_2150	0.73	+2.8
2,457,660	-		PP_2150	0.73	+0.1
2,457,684	-		PP_2150	0.75	+1.4
2,457,893	-				-0.2
2,457,972	+				-0.9
2,457,972	+				-0.9
2,457,972	+				-1.0
2,457,972	+				-0.1
2,457,973	-				+0.3
2,457,973	-				+2.2
2,457,973	-				+2.8
2,457,973	-				+3.4
2,457,973	-				+1.2
2,457,976	+				-0.7
2,457,976	+				-0.2
2,457,976	+				+0.7
2,457,976	+				+0.0
2,457,976	+				-0.5
2,457,977	-				+2.8
2,457,977	-				+1.3
2,457,977	-				+2.8
2,457,977	-				+0.6
2,458,014	+				-0.9
2,458,040	+				+1.0