Strain Fitness in Pseudomonas putida KT2440 around PP_1986

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,251,230	+		PP_1985	0.39	-2.5
2,251,398	+		PP_1985	0.51	-2.3
2,251,409	-		PP_1985	0.52	-0.6
2,251,427	-		PP_1985	0.53	-1.6
2,251,657	+		PP_1985	0.69	-1.4
2,251,657	+		PP_1985	0.69	+1.8
2,251,658	-		PP_1985	0.69	-1.7
2,252,241	+		PP_1986	0.22	+0.0
2,252,242	-		PP_1986	0.22	-0.2
2,252,263	-		PP_1986	0.25	-0.8
2,252,320	-		PP_1986	0.34	-3.0
2,252,403	+		PP_1986	0.47	-2.3
2,252,780	-				-0.4
2,252,784	-				-1.2
2,253,076	+		PP_1987	0.28	+0.3
2,253,385	+		PP_1987	0.68	-1.2
2,253,555	-				-2.9
2,253,562	+				+0.8
2,253,563	-				+0.8
2,253,563	-				+0.5
2,253,563	-				-2.6
2,253,565	+				+1.5