Strain Fitness in Pseudomonas putida KT2440 around PP_1831

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,055,978	+				+0.9
2,055,990	+				-2.0
2,055,990	+				-0.4
2,055,990	+				+0.4
2,055,990	+				-0.8
2,055,991	-				+0.2
2,055,991	-				-0.1
2,055,991	-				-0.6
2,055,991	-				+1.2
2,056,000	+				+0.2
2,056,110	-				+0.9
2,056,130	+				-0.2
2,056,130	+				-1.2
2,056,131	-				-0.4
2,056,131	-				+0.2
2,056,131	-				+0.2
2,056,139	+				-0.1
2,056,139	+				-1.9
2,056,140	-				-0.2
2,056,182	-				-1.1
2,056,370	+		PP_1831	0.24	-0.2
2,056,370	+		PP_1831	0.24	-2.4
2,056,370	+		PP_1831	0.24	+0.5
2,056,371	-		PP_1831	0.24	-0.9
2,056,478	+		PP_1831	0.33	+0.3
2,056,488	+		PP_1831	0.34	-2.2
2,056,488	+		PP_1831	0.34	-1.7
2,056,489	-		PP_1831	0.34	+0.4
2,056,489	-		PP_1831	0.34	-1.3
2,056,489	-		PP_1831	0.34	+0.0
2,056,568	+		PP_1831	0.41	+0.0
2,056,712	+		PP_1831	0.54	-0.4
2,056,713	-		PP_1831	0.54	+0.9
2,056,713	-		PP_1831	0.54	-0.8
2,056,754	+		PP_1831	0.57	+0.5
2,056,754	+		PP_1831	0.57	-1.4
2,056,765	+		PP_1831	0.58	-0.2
2,056,816	-		PP_1831	0.63	-1.2
2,056,816	-		PP_1831	0.63	-0.5
2,056,968	+		PP_1831	0.76	-0.2
2,056,969	-		PP_1831	0.76	-2.2
2,057,085	-		PP_1831	0.86	+0.1
2,057,127	-		PP_1831	0.89	-1.7
2,057,135	+				-0.3
2,057,173	+				+0.2
2,057,174	-				-0.5
2,057,174	-				-0.0
2,057,207	-				-1.3
2,057,329	-		PP_1832	0.15	+1.0
2,057,329	-		PP_1832	0.15	+0.4
2,057,428	+		PP_1832	0.34	-1.2
2,057,602	+		PP_1832	0.66	+0.8
2,057,602	+		PP_1832	0.66	-1.3
2,057,602	+		PP_1832	0.66	+0.0
2,057,603	-		PP_1832	0.66	+2.8
2,057,676	+		PP_1832	0.80	+2.8
2,057,676	+		PP_1832	0.80	+1.1
2,057,676	+		PP_1832	0.80	+1.8
2,057,676	+		PP_1832	0.80	-1.9
2,057,677	-		PP_1832	0.80	+1.1
2,057,677	-		PP_1832	0.80	+1.8
2,057,705	-		PP_1832	0.85	+0.9
2,057,705	-		PP_1832	0.85	+0.5
2,057,705	-		PP_1832	0.85	-0.4
2,057,705	-		PP_1832	0.85	-0.2
2,057,783	-				+0.5
2,057,821	+				-0.2
2,057,822	-				+0.4
2,057,822	-				-0.5
2,057,822	-				-0.4
2,057,842	-				+0.6
2,057,842	-				-0.5
2,057,865	+				-0.4
2,057,865	+				+0.2
2,057,866	-				-0.5
2,057,866	-				-1.6
2,057,904	+				+0.4
2,057,904	+				-1.4
2,057,904	+				+0.5
2,057,904	+				+0.9
2,057,905	-				+0.4
2,057,905	-				-0.8
2,057,905	-				+0.2
2,057,920	-				-1.2
2,058,051	+		PP_1833	0.46	-0.5
2,058,051	+		PP_1833	0.46	-2.1
2,058,098	-		PP_1833	0.61	+0.5
2,058,104	+		PP_1833	0.63	+0.3
2,058,104	+		PP_1833	0.63	+0.5
2,058,130	+		PP_1833	0.72	+1.8
2,058,184	+				+1.0