Strain Fitness in Pseudomonas putida KT2440 around PP_1539

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,741,050	+		PP_5472	0.56	+0.0
1,741,050	+		PP_5472	0.56	-0.3
1,741,050	+		PP_5472	0.56	-0.0
1,741,051	-		PP_5472	0.56	-0.5
1,741,051	-		PP_5472	0.56	+0.8
1,741,051	-		PP_5472	0.56	+2.1
1,741,054	+		PP_5472	0.57	-0.1
1,741,054	+		PP_5472	0.57	+0.3
1,741,055	-		PP_5472	0.58	-0.9
1,741,055	-		PP_5472	0.58	-0.3
1,741,063	-		PP_5472	0.61	+1.1
1,741,308	+		PP_1538	0.20	-0.7
1,741,308	+		PP_1538	0.20	+0.1
1,741,309	-		PP_1538	0.20	-0.9
1,741,309	-		PP_1538	0.20	+0.0
1,741,309	-		PP_1538	0.20	+0.3
1,741,309	-		PP_1538	0.20	+3.8
1,741,311	+		PP_1538	0.20	-1.9
1,741,311	+		PP_1538	0.20	+0.0
1,741,312	-		PP_1538	0.20	+0.9
1,741,312	-		PP_1538	0.20	-0.5
1,741,343	+		PP_1538	0.24	+0.2
1,741,540	-		PP_1538	0.50	-0.2
1,741,560	+		PP_1538	0.53	-1.5
1,741,561	-		PP_1538	0.53	-0.2
1,741,624	+		PP_1538	0.62	+0.4
1,741,701	+		PP_1538	0.72	+1.2
1,741,760	+		PP_1538	0.80	-1.2
1,741,760	+		PP_1538	0.80	+0.2
1,741,760	+		PP_1538	0.80	-0.8
1,741,761	-		PP_1538	0.80	-1.4
1,741,761	-		PP_1538	0.80	-0.1
1,741,761	-		PP_1538	0.80	+0.5
1,741,761	-		PP_1538	0.80	+1.5
1,741,761	-		PP_1538	0.80	-0.4
1,741,761	-		PP_1538	0.80	+0.2
1,741,912	+				+0.8
1,741,912	+				+0.3
1,741,912	+				+0.2
1,741,912	+				-1.6
1,741,913	-				-1.0
1,741,913	-				-0.5
1,741,947	+				-2.3
1,741,947	+				-0.4
1,741,947	+				-1.4
1,741,947	+				-0.1
1,741,948	-				+0.2
1,742,050	+				+1.1
1,742,050	+				-0.0
1,742,050	+				-0.0
1,742,051	-				-0.9
1,742,091	+		PP_1539	0.11	-0.6
1,742,092	-		PP_1539	0.11	+1.5
1,742,092	-		PP_1539	0.11	+0.1
1,742,092	-		PP_1539	0.11	+0.5
1,742,092	-		PP_1539	0.11	+1.3
1,742,181	+		PP_1539	0.21	+0.3
1,742,182	-		PP_1539	0.21	+1.6
1,742,182	-		PP_1539	0.21	+0.6
1,742,206	+		PP_1539	0.24	+1.0
1,742,253	+		PP_1539	0.30	+1.8
1,742,254	-		PP_1539	0.30	+0.7
1,742,258	+		PP_1539	0.30	-0.0
1,742,258	+		PP_1539	0.30	-0.3
1,742,259	-		PP_1539	0.30	+0.7
1,742,259	-		PP_1539	0.30	-1.4
1,742,259	-		PP_1539	0.30	+0.6
1,742,272	-		PP_1539	0.32	+1.3
1,742,272	-		PP_1539	0.32	-0.0
1,742,334	+		PP_1539	0.39	+0.2
1,742,402	+		PP_1539	0.47	+0.3
1,742,402	+		PP_1539	0.47	+0.8
1,742,402	+		PP_1539	0.47	+0.3
1,742,403	-		PP_1539	0.47	+0.9
1,742,403	-		PP_1539	0.47	-0.0
1,742,403	-		PP_1539	0.47	+0.8
1,742,403	-		PP_1539	0.47	-0.5
1,742,418	+		PP_1539	0.49	-0.6
1,742,418	+		PP_1539	0.49	+0.5
1,742,432	-		PP_1539	0.50	-0.0
1,742,468	+		PP_1539	0.54	+0.6
1,742,469	-		PP_1539	0.55	-0.7
1,742,469	-		PP_1539	0.55	+0.7
1,742,587	-		PP_1539	0.68	-1.6
1,742,635	-		PP_1539	0.74	-0.9
1,742,708	+		PP_1539	0.82	-1.4
1,742,756	+		PP_1539	0.88	-0.1
1,742,756	+		PP_1539	0.88	-0.1
1,742,756	+		PP_1539	0.88	+0.9
1,742,756	+		PP_1539	0.88	+2.0
1,742,757	-		PP_1539	0.88	-0.3
1,742,757	-		PP_1539	0.88	+0.6
1,742,757	-		PP_1539	0.88	-0.3
1,742,757	-		PP_1539	0.88	-0.2
1,742,757	-		PP_1539	0.88	-1.1
1,742,779	-				+1.3
1,742,805	+				-0.2
1,742,805	+				+0.3
1,742,806	-				+1.2
1,743,155	+				+0.4
1,743,155	+				-1.1
1,743,315	+				-0.4
1,743,727	+		PP_1541	0.47	+1.5
1,743,728	-		PP_1541	0.47	-0.4
1,743,793	+		PP_1541	0.55	-0.7
1,743,793	+		PP_1541	0.55	+0.9
1,743,794	-		PP_1541	0.55	+0.9
1,743,794	-		PP_1541	0.55	-0.4
1,743,819	+		PP_1541	0.57	-0.3
1,743,819	+		PP_1541	0.57	+0.2
1,743,819	+		PP_1541	0.57	+1.0
1,743,820	-		PP_1541	0.57	-0.8
1,743,820	-		PP_1541	0.57	+0.2