Strain Fitness in Pseudomonas putida KT2440 around PP_1421

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,620,190	-				-0.5
1,620,261	-				+0.3
1,620,318	+				-0.5
1,620,318	+				+1.6
1,620,318	+				-0.6
1,620,318	+				-1.4
1,620,394	+				+0.5
1,620,445	+				-1.5
1,620,446	-				+0.3
1,620,446	-				+0.0
1,620,514	+		PP_1420	0.12	+0.4
1,620,514	+		PP_1420	0.12	-0.5
1,620,514	+		PP_1420	0.12	-0.8
1,620,515	-		PP_1420	0.12	-0.4
1,620,515	-		PP_1420	0.12	-0.5
1,620,515	-		PP_1420	0.12	-0.4
1,620,567	+		PP_1420	0.20	-0.5
1,620,567	+		PP_1420	0.20	-0.2
1,620,567	+		PP_1420	0.20	+0.6
1,620,568	-		PP_1420	0.20	-0.2
1,620,619	+		PP_1420	0.27	-0.7
1,620,619	+		PP_1420	0.27	+1.1
1,620,620	-		PP_1420	0.28	+0.4
1,620,662	+		PP_1420	0.34	-1.1
1,620,663	-		PP_1420	0.34	-0.1
1,620,663	-		PP_1420	0.34	-0.7
1,620,782	-		PP_1420	0.52	+0.1
1,620,782	-		PP_1420	0.52	+0.1
1,620,782	-		PP_1420	0.52	+1.8
1,620,834	+		PP_1420	0.59	-0.3
1,620,834	+		PP_1420	0.59	+1.2
1,620,834	+		PP_1420	0.59	+0.4
1,620,835	-		PP_1420	0.60	+0.8
1,621,011	+		PP_1420	0.86	-0.6
1,621,137	+				+1.8
1,621,182	+				-1.3
1,621,183	-				+0.3
1,621,223	-				+0.6
1,621,223	-				-0.2
1,621,306	+		PP_1421	0.16	-0.3
1,621,307	-		PP_1421	0.16	-0.6
1,621,307	-		PP_1421	0.16	+0.0
1,621,330	+		PP_1421	0.17	-0.4
1,621,331	-		PP_1421	0.18	-0.1
1,621,331	-		PP_1421	0.18	-1.0
1,621,331	-		PP_1421	0.18	+0.2
1,621,351	+		PP_1421	0.19	-0.8
1,621,351	+		PP_1421	0.19	+0.3
1,621,352	-		PP_1421	0.19	+0.1
1,621,355	-		PP_1421	0.19	-0.4
1,621,463	-		PP_1421	0.27	-0.1
1,621,463	-		PP_1421	0.27	-0.8
1,621,474	+		PP_1421	0.28	-0.2
1,621,474	+		PP_1421	0.28	-0.5
1,621,474	+		PP_1421	0.28	-0.1
1,621,474	+		PP_1421	0.28	+0.1
1,621,475	-		PP_1421	0.28	-0.4
1,622,226	+		PP_1421	0.82	-0.7
1,622,226	+		PP_1421	0.82	+1.5
1,622,227	-		PP_1421	0.82	-0.0
1,622,301	+		PP_1421	0.87	-0.3
1,622,341	+				-0.6
1,622,341	+				+0.2
1,622,467	-				+0.7
1,622,539	-				+0.3
1,622,696	-		PP_1422	0.19	+0.7
1,622,821	+		PP_1422	0.34	-2.3
1,622,821	+		PP_1422	0.34	+0.4
1,622,821	+		PP_1422	0.34	-0.5
1,622,822	-		PP_1422	0.34	-0.9
1,622,869	+		PP_1422	0.40	+0.5
1,622,869	+		PP_1422	0.40	+0.1
1,622,869	+		PP_1422	0.40	-0.4
1,622,869	+		PP_1422	0.40	-1.2
1,622,869	+		PP_1422	0.40	+1.8
1,622,870	-		PP_1422	0.40	-0.2
1,622,877	+		PP_1422	0.41	+0.3
1,623,082	+		PP_1422	0.66	+0.8
1,623,220	-		PP_1422	0.83	-1.5
1,623,360	+				+2.2
1,623,360	+				+0.1
1,623,441	-				-1.9
1,623,441	-				-0.5