Strain Fitness in Pseudomonas putida KT2440 around PP_1253

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,431,448	+				-0.7
1,431,483	+				+1.8
1,431,498	+				-0.2
1,431,499	-				-0.5
1,431,534	+				-2.6
1,431,534	+				-0.2
1,431,610	+		PP_1253	0.22	+1.0
1,431,780	+		PP_1253	0.73	+1.2
1,431,780	+		PP_1253	0.73	-0.3
1,431,781	-		PP_1253	0.73	-0.7
1,431,781	-		PP_1253	0.73	+1.2
1,431,781	-		PP_1253	0.73	+0.1
1,431,823	+		PP_1253	0.85	-0.8
1,431,823	+		PP_1253	0.85	+0.1
1,431,823	+		PP_1253	0.85	-1.0
1,431,823	+		PP_1253	0.85	+1.2
1,431,823	+		PP_1253	0.85	+0.8
1,431,824	-		PP_1253	0.86	+0.7
1,431,824	-		PP_1253	0.86	-0.8
1,431,825	+		PP_1253	0.86	-0.6
1,431,825	+		PP_1253	0.86	-2.2
1,431,825	+		PP_1253	0.86	-0.1
1,431,826	-		PP_1253	0.86	+0.1
1,431,826	-		PP_1253	0.86	-1.9
1,431,826	-		PP_1253	0.86	-0.4
1,431,848	-				+0.0
1,431,942	+				+2.8
1,431,942	+				-0.1
1,431,943	-				-2.5
1,431,969	-				-0.2
1,431,999	+				+0.2
1,431,999	+				-0.4
1,431,999	+				-0.3
1,431,999	+				-0.4
1,431,999	+				-0.1
1,431,999	+				+1.2
1,431,999	+				+0.1
1,432,000	-				+0.9
1,432,000	-				+2.0
1,432,000	-				+0.6
1,432,000	-				-0.4
1,432,115	+		PP_1254	0.18	-1.5
1,432,115	+		PP_1254	0.18	-0.9
1,432,115	+		PP_1254	0.18	+0.3
1,432,116	-		PP_1254	0.18	-1.5
1,432,137	-		PP_1254	0.20	-1.8
1,432,444	+		PP_1254	0.48	+0.9
1,432,445	-		PP_1254	0.48	-0.7
1,432,467	+		PP_1254	0.50	-0.5
1,432,468	-		PP_1254	0.50	-1.7