Strain Fitness in Pseudomonas putida KT2440 around PP_1228

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,402,759	+		PP_1227	0.19	+0.4
1,402,760	-		PP_1227	0.19	-1.8
1,402,769	-		PP_1227	0.20	-0.2
1,402,815	+		PP_1227	0.25	+1.1
1,402,815	+		PP_1227	0.25	-0.3
1,402,815	+		PP_1227	0.25	-0.7
1,402,816	-		PP_1227	0.25	-1.2
1,402,816	-		PP_1227	0.25	+0.5
1,402,934	+		PP_1227	0.37	+1.6
1,402,936	+		PP_1227	0.38	+0.2
1,402,936	+		PP_1227	0.38	+0.5
1,402,936	+		PP_1227	0.38	-2.0
1,402,936	+		PP_1227	0.38	-0.5
1,402,936	+		PP_1227	0.38	+1.0
1,402,936	+		PP_1227	0.38	-1.7
1,402,936	+		PP_1227	0.38	-1.0
1,402,936	+		PP_1227	0.38	-0.0
1,402,937	-		PP_1227	0.38	+1.0
1,402,937	-		PP_1227	0.38	-0.2
1,402,937	-		PP_1227	0.38	+0.2
1,403,203	+		PP_1227	0.65	-2.0
1,403,204	-		PP_1227	0.66	-0.1
1,403,204	-		PP_1227	0.66	-1.0
1,403,204	-		PP_1227	0.66	-0.2
1,403,244	+		PP_1227	0.70	+1.4
1,403,244	+		PP_1227	0.70	+0.8
1,403,385	+		PP_1227	0.84	-0.2
1,403,385	+		PP_1227	0.84	-0.1
1,403,478	+				-1.5
1,403,479	-				-1.5
1,403,479	-				+1.4
1,403,528	+				-0.2
1,403,528	+				-0.8
1,403,534	+				-0.2
1,403,535	-				+0.7
1,403,535	-				+0.0
1,403,552	-				+0.6
1,403,552	-				+0.6
1,403,552	-				-1.2
1,403,616	+				-1.9
1,403,616	+				-0.3
1,403,792	+				-1.3
1,403,793	-				+0.9
1,403,837	-		PP_1228	0.11	-1.3
1,404,334	+		PP_1228	0.35	-1.1
1,404,542	-		PP_1228	0.45	-0.8
1,404,662	+		PP_1228	0.51	+0.5
1,404,764	+		PP_1228	0.56	+1.3
1,405,004	+		PP_1228	0.67	-0.3
1,405,005	-		PP_1228	0.67	-1.0
1,405,005	-		PP_1228	0.67	-2.3
1,405,227	+		PP_1228	0.78	-0.1
1,405,278	+		PP_1228	0.80	+0.4
1,405,278	+		PP_1228	0.80	-0.2
1,405,279	-		PP_1228	0.81	+1.8
1,405,324	-		PP_1228	0.83	-0.3
1,405,421	+		PP_1228	0.87	-1.0
1,405,421	+		PP_1228	0.87	-0.3
1,405,422	-		PP_1228	0.87	-0.4
1,405,453	-		PP_1228	0.89	-0.9
1,405,467	+		PP_1228	0.90	-0.7
1,405,761	+				-0.2
1,405,761	+				-0.1
1,405,762	-				-0.1
1,405,762	-				-0.4
1,405,806	+				-1.3
1,405,832	-				-0.0
1,405,908	-		PP_1229	0.12	-0.7
1,405,914	+		PP_1229	0.12	+0.2
1,405,914	+		PP_1229	0.12	-1.5
1,405,915	-		PP_1229	0.12	+0.2
1,405,915	-		PP_1229	0.12	-0.2
1,405,939	+		PP_1229	0.14	+0.5
1,405,939	+		PP_1229	0.14	+0.3
1,405,939	+		PP_1229	0.14	-1.0
1,405,959	+		PP_1229	0.16	-0.5
1,405,959	+		PP_1229	0.16	-1.5
1,405,960	-		PP_1229	0.16	+0.2
1,406,076	-		PP_1229	0.24	+0.3
1,406,162	+		PP_1229	0.31	+0.8
1,406,162	+		PP_1229	0.31	-0.1
1,406,162	+		PP_1229	0.31	+0.7
1,406,163	-		PP_1229	0.31	+1.1
1,406,166	+		PP_1229	0.31	-0.8
1,406,166	+		PP_1229	0.31	-0.7
1,406,166	+		PP_1229	0.31	+0.3
1,406,166	+		PP_1229	0.31	+0.7
1,406,166	+		PP_1229	0.31	-1.2
1,406,167	-		PP_1229	0.31	+0.3
1,406,167	-		PP_1229	0.31	+0.3
1,406,268	+		PP_1229	0.38	+0.1
1,406,269	-		PP_1229	0.39	-0.2
1,406,328	+		PP_1229	0.43	+0.8
1,406,328	+		PP_1229	0.43	-0.2
1,406,328	+		PP_1229	0.43	-0.1
1,406,329	-		PP_1229	0.43	-2.0
1,406,329	-		PP_1229	0.43	-0.7
1,406,343	+				+2.8
1,406,343	+		PP_1229	0.44	-0.5
1,406,344	-		PP_1229	0.44	-0.3
1,406,579	+		PP_1229	0.61	-0.0
1,406,589	+		PP_1229	0.62	-0.1
1,406,590	-		PP_1229	0.62	-1.4