Strain Fitness in Pseudomonas putida KT2440 around PP_0996

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,133,363	+				+1.2
1,133,364	-		PP_0994	0.89	-0.8
1,133,536	+		PP_0995	0.19	+0.2
1,133,536	+		PP_0995	0.19	-2.0
1,133,536	+		PP_0995	0.19	-1.3
1,133,536	+		PP_0995	0.19	-1.0
1,133,537	-		PP_0995	0.19	+2.7
1,133,537	-		PP_0995	0.19	-1.3
1,133,539	+		PP_0995	0.19	+1.2
1,133,540	-		PP_0995	0.20	-1.7
1,133,540	-		PP_0995	0.20	+0.6
1,133,540	-		PP_0995	0.20	-0.8
1,133,563	+		PP_0995	0.23	-1.1
1,133,797	+		PP_0995	0.60	-1.2
1,133,812	+		PP_0995	0.62	-1.9
1,133,987	+		PP_0995	0.89	-0.7
1,134,053	+				-0.4
1,134,054	-				-0.4
1,134,054	-				+0.5
1,134,135	-				+1.6
1,134,164	+				-1.0
1,134,164	+				+0.0
1,134,252	-				-0.6
1,134,294	+		PP_0996	0.13	+1.7
1,134,295	-		PP_0996	0.13	-1.0
1,134,295	-		PP_0996	0.13	+0.1
1,134,295	-		PP_0996	0.13	+1.5
1,134,359	-		PP_0996	0.19	-2.2
1,134,359	-		PP_0996	0.19	+0.5
1,134,420	+		PP_0996	0.24	+0.4
1,134,421	-		PP_0996	0.24	+0.9
1,134,421	-		PP_0996	0.24	-0.0
1,134,430	+		PP_0996	0.25	-0.4
1,134,431	-		PP_0996	0.25	-1.3
1,134,540	-		PP_0996	0.34	+1.7
1,134,575	-		PP_0996	0.37	+0.7
1,134,575	-		PP_0996	0.37	+0.4
1,134,589	+		PP_0996	0.38	-1.6
1,134,640	-		PP_0996	0.42	+1.0
1,134,643	+		PP_0996	0.42	+0.6
1,134,644	-		PP_0996	0.42	+0.1
1,134,672	+		PP_0996	0.45	+0.5
1,134,672	+		PP_0996	0.45	-0.7
1,134,672	+		PP_0996	0.45	+0.5
1,134,673	-		PP_0996	0.45	+0.8
1,134,673	-		PP_0996	0.45	-0.8
1,134,673	-		PP_0996	0.45	-1.6
1,134,688	+		PP_0996	0.46	-0.5
1,134,688	+		PP_0996	0.46	+0.4
1,135,027	+		PP_0996	0.74	+0.0
1,135,027	+		PP_0996	0.74	+1.7
1,135,027	+		PP_0996	0.74	-0.2
1,135,028	-		PP_0996	0.74	-0.2
1,135,114	-		PP_0996	0.81	+0.6
1,135,241	+				-0.8
1,135,241	+				-0.4
1,135,241	+				-1.9
1,135,242	-				+1.7
1,135,242	-				+0.6
1,135,242	-				+2.7
1,135,285	+				-2.2
1,135,285	+				+0.4
1,135,306	+				+0.7
1,135,306	+				-0.9
1,135,306	+				+0.6
1,135,306	+				+0.9
1,135,467	+				+0.1
1,135,492	+				+0.4
1,135,492	+				+1.8
1,135,493	-				-2.1
1,135,560	+				-0.7
1,135,560	+				+0.4
1,135,561	-				+0.2
1,135,569	+				-1.0
1,135,569	+				+0.1
1,135,571	+				-0.3
1,135,572	-				-0.3
1,135,581	-				-0.2
1,135,604	+				-0.3
1,135,604	+				-0.7
1,135,604	+				-0.6
1,135,605	-				+0.5
1,135,605	-				+0.4
1,135,705	+		PP_0997	0.16	-1.6
1,135,706	-		PP_0997	0.16	+0.6
1,135,889	+		PP_0997	0.28	-0.4
1,135,890	-		PP_0997	0.28	-0.4
1,135,918	-		PP_0997	0.30	-0.3
1,136,073	+		PP_0997	0.40	+0.4