Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0816 and PP_0817 are separated by 312 nucleotides PP_0817 and PP_0818 are separated by 113 nucleotides
PP_0816: PP_0816 - protoheme IX farnesyltransferase, at 954,159 to 955,046
_0816
PP_0817: PP_0817 - aminotransferase, at 955,359 to 956,567
_0817
PP_0818: PP_0818 - conserved protein of unknown function, at 956,681 to 957,808
_0818
Position (kb)
955
956
957 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 955.035 kb on + strand at 955.036 kb on - strand at 955.036 kb on - strand at 955.036 kb on - strand at 955.036 kb on - strand at 955.145 kb on + strand at 955.145 kb on + strand at 955.145 kb on + strand at 955.159 kb on - strand at 955.159 kb on - strand at 955.340 kb on + strand at 955.340 kb on + strand at 955.340 kb on + strand at 955.341 kb on - strand at 955.414 kb on - strand at 955.534 kb on + strand, within PP_0817 at 955.535 kb on - strand, within PP_0817 at 955.535 kb on - strand, within PP_0817 at 955.618 kb on - strand, within PP_0817 at 955.618 kb on - strand, within PP_0817 at 955.630 kb on - strand, within PP_0817 at 955.727 kb on + strand, within PP_0817 at 955.768 kb on - strand, within PP_0817 at 955.965 kb on + strand, within PP_0817 at 955.965 kb on + strand, within PP_0817 at 955.966 kb on - strand, within PP_0817 at 955.966 kb on - strand, within PP_0817 at 955.966 kb on - strand, within PP_0817 at 955.972 kb on + strand, within PP_0817 at 955.972 kb on + strand, within PP_0817 at 955.973 kb on - strand, within PP_0817 at 955.973 kb on - strand, within PP_0817 at 955.995 kb on + strand, within PP_0817 at 956.094 kb on + strand, within PP_0817 at 956.094 kb on + strand, within PP_0817 at 956.094 kb on + strand, within PP_0817 at 956.172 kb on + strand, within PP_0817 at 956.172 kb on + strand, within PP_0817 at 956.172 kb on + strand, within PP_0817 at 956.343 kb on + strand, within PP_0817 at 956.343 kb on + strand, within PP_0817 at 956.343 kb on + strand, within PP_0817 at 956.343 kb on + strand, within PP_0817 at 956.370 kb on + strand, within PP_0817 at 956.458 kb on - strand at 956.565 kb on + strand at 956.645 kb on - strand at 956.782 kb on + strand at 956.996 kb on + strand, within PP_0818 at 956.997 kb on - strand, within PP_0818 at 957.068 kb on + strand, within PP_0818 at 957.240 kb on + strand, within PP_0818 at 957.252 kb on + strand, within PP_0818 at 957.252 kb on + strand, within PP_0818 at 957.252 kb on + strand, within PP_0818 at 957.252 kb on + strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.253 kb on - strand, within PP_0818 at 957.288 kb on + strand, within PP_0818 at 957.288 kb on + strand, within PP_0818 at 957.289 kb on - strand, within PP_0818 at 957.317 kb on - strand, within PP_0818 at 957.317 kb on - strand, within PP_0818 at 957.330 kb on - strand, within PP_0818 at 957.330 kb on - strand, within PP_0818 at 957.330 kb on - strand, within PP_0818 at 957.333 kb on + strand, within PP_0818 at 957.333 kb on + strand, within PP_0818 at 957.334 kb on - strand, within PP_0818 at 957.334 kb on - strand, within PP_0818 at 957.334 kb on - strand, within PP_0818 at 957.334 kb on - strand, within PP_0818 at 957.341 kb on + strand, within PP_0818 at 957.342 kb on - strand, within PP_0818 at 957.409 kb on - strand, within PP_0818 at 957.409 kb on - strand, within PP_0818
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 955,035 + -1.2 955,036 - -0.3 955,036 - +1.3 955,036 - +2.7 955,036 - +3.1 955,145 + -0.2 955,145 + -0.2 955,145 + -0.7 955,159 - -1.3 955,159 - +0.0 955,340 + -0.7 955,340 + +2.1 955,340 + +0.3 955,341 - -0.9 955,414 - +0.0 955,534 + PP_0817 0.14 +3.7 955,535 - PP_0817 0.15 +0.8 955,535 - PP_0817 0.15 +0.6 955,618 - PP_0817 0.21 +0.8 955,618 - PP_0817 0.21 -0.8 955,630 - PP_0817 0.22 +2.7 955,727 + PP_0817 0.30 -0.7 955,768 - PP_0817 0.34 +0.2 955,965 + PP_0817 0.50 +1.4 955,965 + PP_0817 0.50 +0.6 955,966 - PP_0817 0.50 +0.7 955,966 - PP_0817 0.50 +0.9 955,966 - PP_0817 0.50 +0.8 955,972 + PP_0817 0.51 -0.2 955,972 + PP_0817 0.51 +1.3 955,973 - PP_0817 0.51 -0.0 955,973 - PP_0817 0.51 -0.4 955,995 + PP_0817 0.53 -0.4 956,094 + PP_0817 0.61 +0.3 956,094 + PP_0817 0.61 +0.4 956,094 + PP_0817 0.61 +0.4 956,172 + PP_0817 0.67 -0.7 956,172 + PP_0817 0.67 +0.8 956,172 + PP_0817 0.67 +0.4 956,343 + PP_0817 0.81 -0.3 956,343 + PP_0817 0.81 +3.3 956,343 + PP_0817 0.81 +2.1 956,343 + PP_0817 0.81 +1.7 956,370 + PP_0817 0.84 -1.0 956,458 - +0.1 956,565 + -2.1 956,645 - -1.4 956,782 + +0.7 956,996 + PP_0818 0.28 +1.0 956,997 - PP_0818 0.28 -0.9 957,068 + PP_0818 0.34 -1.8 957,240 + PP_0818 0.50 -0.0 957,252 + PP_0818 0.51 -1.6 957,252 + PP_0818 0.51 -0.3 957,252 + PP_0818 0.51 +0.7 957,252 + PP_0818 0.51 -0.3 957,253 - PP_0818 0.51 +0.0 957,253 - PP_0818 0.51 -0.4 957,253 - PP_0818 0.51 +1.1 957,253 - PP_0818 0.51 -0.3 957,253 - PP_0818 0.51 +1.1 957,253 - PP_0818 0.51 +0.7 957,253 - PP_0818 0.51 +0.1 957,288 + PP_0818 0.54 -0.9 957,288 + PP_0818 0.54 +0.2 957,289 - PP_0818 0.54 -0.2 957,317 - PP_0818 0.56 -0.3 957,317 - PP_0818 0.56 +1.8 957,330 - PP_0818 0.58 +0.6 957,330 - PP_0818 0.58 -0.5 957,330 - PP_0818 0.58 +0.1 957,333 + PP_0818 0.58 -1.2 957,333 + PP_0818 0.58 +0.5 957,334 - PP_0818 0.58 +0.4 957,334 - PP_0818 0.58 -0.1 957,334 - PP_0818 0.58 -2.1 957,334 - PP_0818 0.58 -0.0 957,341 + PP_0818 0.59 -0.6 957,342 - PP_0818 0.59 +0.2 957,409 - PP_0818 0.65 -1.1 957,409 - PP_0818 0.65 +0.6
Or see this region's nucleotide sequence