Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0786 and PP_0787 are separated by 75 nucleotides PP_0787 and PP_0788 are separated by 243 nucleotides
PP_0786: PP_0786 - thioredoxin reductase, at 901,612 to 902,574
_0786
PP_0787: PP_0787 - quinolinate phosphoribosyltransferase (decarboxylating) monomer, at 902,650 to 903,498
_0787
PP_0788: PP_0788 - conserved protein of unknown function, at 903,742 to 905,901
_0788
Position (kb) 902
903
904 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.800 kb on - strand, within PP_0787 at 903.008 kb on + strand, within PP_0787 at 903.009 kb on - strand, within PP_0787 at 903.016 kb on + strand, within PP_0787 at 903.016 kb on + strand, within PP_0787 at 903.017 kb on - strand, within PP_0787 at 903.487 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.530 kb on - strand at 903.531 kb on + strand at 903.531 kb on + strand at 903.532 kb on - strand at 903.551 kb on + strand at 903.551 kb on + strand at 903.551 kb on + strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.641 kb on - strand at 903.641 kb on - strand at 903.641 kb on - strand at 903.644 kb on + strand at 903.645 kb on - strand at 903.645 kb on - strand at 903.675 kb on + strand at 903.687 kb on + strand at 903.799 kb on - strand at 903.799 kb on - strand at 903.953 kb on - strand at 903.953 kb on - strand at 903.953 kb on - strand at 904.014 kb on + strand, within PP_0788 at 904.015 kb on - strand, within PP_0788 at 904.015 kb on - strand, within PP_0788 at 904.213 kb on + strand, within PP_0788 at 904.214 kb on - strand, within PP_0788 at 904.255 kb on + strand, within PP_0788 at 904.255 kb on + strand, within PP_0788 at 904.275 kb on + strand, within PP_0788 at 904.275 kb on + strand, within PP_0788 at 904.330 kb on + strand, within PP_0788 at 904.331 kb on - strand, within PP_0788 at 904.331 kb on - strand, within PP_0788 at 904.357 kb on + strand, within PP_0788 at 904.358 kb on - strand, within PP_0788 at 904.395 kb on - strand, within PP_0788
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 902,717 + -3.3 902,717 + -2.9 902,717 + -2.0 902,717 + -0.2 902,717 + -3.1 902,717 + -2.6 902,717 + +0.2 902,717 + -2.5 902,717 + -1.1 902,718 - -0.5 902,718 - -1.8 902,718 - +2.7 902,718 - -2.0 902,800 - PP_0787 0.18 -2.2 903,008 + PP_0787 0.42 -3.3 903,009 - PP_0787 0.42 -3.0 903,016 + PP_0787 0.43 -1.3 903,016 + PP_0787 0.43 -0.6 903,017 - PP_0787 0.43 -1.9 903,487 + -0.4 903,515 + -1.2 903,515 + -1.8 903,515 + -0.2 903,515 + -0.1 903,515 + -0.1 903,516 - -0.1 903,516 - +0.3 903,516 - -0.0 903,516 - +0.1 903,530 - -0.8 903,531 + +1.7 903,531 + +0.2 903,532 - +0.7 903,551 + -0.3 903,551 + -0.8 903,551 + +0.7 903,552 - +0.6 903,552 - -0.2 903,552 - -0.1 903,552 - -0.5 903,640 + -0.5 903,640 + -1.0 903,640 + +1.2 903,640 + +0.9 903,640 + -0.5 903,641 - +0.7 903,641 - +0.4 903,641 - +0.5 903,644 + +0.4 903,645 - -1.0 903,645 - -0.5 903,675 + +3.7 903,687 + +1.5 903,799 - -1.8 903,799 - +2.7 903,953 - -1.8 903,953 - -0.1 903,953 - -1.3 904,014 + PP_0788 0.13 +0.7 904,015 - PP_0788 0.13 +1.7 904,015 - PP_0788 0.13 -0.6 904,213 + PP_0788 0.22 -0.2 904,214 - PP_0788 0.22 +1.9 904,255 + PP_0788 0.24 -0.2 904,255 + PP_0788 0.24 +0.9 904,275 + PP_0788 0.25 -0.5 904,275 + PP_0788 0.25 -1.6 904,330 + PP_0788 0.27 +0.0 904,331 - PP_0788 0.27 -0.7 904,331 - PP_0788 0.27 +1.5 904,357 + PP_0788 0.28 +1.7 904,358 - PP_0788 0.29 +0.1 904,395 - PP_0788 0.30 -0.3
Or see this region's nucleotide sequence