Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0768 and PP_0769 overlap by 4 nucleotides PP_0769 and PP_0770 are separated by 193 nucleotides PP_0770 and PP_0771 overlap by 4 nucleotides PP_0771 and PP_0772 are separated by 18 nucleotides
PP_0768: PP_0768 - Response regulator/TPR domain protein, at 886,967 to 888,574
_0768
PP_0769: PP_0769 - Sensor histidine kinase, at 888,571 to 889,263
_0769
PP_0770: PP_0770 - PemI-like protein, at 889,457 to 889,711
_0770
PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055
_0771
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
Position (kb) 889
890 Strain fitness (log2 ratio) -2
-1
0
1
2 at 888.530 kb on + strand at 888.779 kb on - strand, within PP_0769 at 888.779 kb on - strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.817 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.151 kb on - strand, within PP_0769 at 889.211 kb on + strand at 889.211 kb on + strand at 889.211 kb on + strand at 889.257 kb on - strand at 889.278 kb on + strand at 889.278 kb on + strand at 889.324 kb on + strand at 889.324 kb on + strand at 889.382 kb on + strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.387 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.440 kb on + strand at 889.441 kb on - strand at 889.452 kb on + strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.515 kb on + strand, within PP_0770 at 889.610 kb on + strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.805 kb on + strand, within PP_0771 at 889.806 kb on - strand, within PP_0771 at 889.810 kb on - strand, within PP_0771 at 889.844 kb on + strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.991 kb on + strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 890.067 kb on - strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.080 kb on - strand at 890.080 kb on - strand at 890.392 kb on + strand, within PP_0772 at 890.392 kb on + strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.470 kb on - strand, within PP_0772
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 888,530 + +1.0 888,779 - PP_0769 0.30 +1.0 888,779 - PP_0769 0.30 -0.6 888,806 + PP_0769 0.34 -0.6 888,806 + PP_0769 0.34 -0.3 888,817 + PP_0769 0.35 +0.5 888,869 + PP_0769 0.43 +0.7 888,869 + PP_0769 0.43 -1.0 888,870 - PP_0769 0.43 -0.6 888,870 - PP_0769 0.43 -0.4 889,103 - PP_0769 0.77 -1.2 889,103 - PP_0769 0.77 +1.1 889,103 - PP_0769 0.77 +2.4 889,151 - PP_0769 0.84 +0.5 889,211 + +0.6 889,211 + -1.3 889,211 + -0.9 889,257 - +0.4 889,278 + -0.9 889,278 + -0.0 889,324 + -0.2 889,324 + -0.7 889,382 + +0.4 889,383 - +0.4 889,383 - +1.2 889,383 - +0.3 889,383 - +0.8 889,387 - +0.7 889,430 - +0.2 889,430 - +0.9 889,432 + +0.1 889,432 + +1.1 889,432 + +0.1 889,432 + +0.0 889,432 + +0.5 889,432 + +1.4 889,433 - -0.8 889,433 - -0.1 889,433 - -0.7 889,433 - -0.0 889,433 - +1.0 889,433 - -0.8 889,433 - +0.1 889,433 - -0.8 889,433 - +0.9 889,436 + +1.8 889,436 + +0.5 889,436 + +0.4 889,436 + -0.1 889,436 + +0.2 889,436 + -1.9 889,436 + +0.3 889,437 - -0.1 889,437 - -0.4 889,437 - -0.2 889,437 - +0.6 889,437 - +0.4 889,437 - -2.6 889,437 - +0.2 889,437 - -0.1 889,437 - +0.4 889,437 - -1.2 889,440 + +0.7 889,441 - -1.2 889,452 + -0.6 889,453 - -0.9 889,453 - -0.1 889,453 - -1.1 889,515 + PP_0770 0.23 -1.4 889,610 + PP_0770 0.60 -1.3 889,611 - PP_0770 0.60 -0.9 889,611 - PP_0770 0.60 -0.2 889,805 + PP_0771 0.28 -1.0 889,806 - PP_0771 0.28 +0.0 889,810 - PP_0771 0.29 -0.3 889,844 + PP_0771 0.39 -1.8 889,845 - PP_0771 0.39 +0.3 889,845 - PP_0771 0.39 +1.0 889,991 + PP_0771 0.81 -0.4 889,992 - PP_0771 0.82 -1.5 889,992 - PP_0771 0.82 -0.3 890,067 - -0.1 890,079 + +0.9 890,079 + +1.6 890,079 + +1.0 890,079 + +0.5 890,080 - +1.0 890,080 - -0.1 890,392 + PP_0772 0.49 -0.1 890,392 + PP_0772 0.49 +0.8 890,393 - PP_0772 0.49 -0.5 890,393 - PP_0772 0.49 -0.2 890,393 - PP_0772 0.49 +0.1 890,469 + PP_0772 0.61 -0.1 890,469 + PP_0772 0.61 -0.0 890,470 - PP_0772 0.61 -0.3 890,470 - PP_0772 0.61 -1.0
Or see this region's nucleotide sequence