Strain Fitness in Pseudomonas putida KT2440 around PP_0530

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0528 and PP_0529 overlap by 4 nucleotidesPP_0529 and PP_0530 are separated by 165 nucleotidesPP_0530 and PP_0531 are separated by 371 nucleotides PP_0528: PP_0528 - farnesyl diphosphate synthase, at 614,359 to 615,246 _0528 PP_0529: PP_0529 - Exodeoxyribonuclease 7 small subunit, at 615,243 to 615,485 _0529 PP_0530: PP_0530 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 615,651 to 616,301 _0530 PP_0531: PP_0531 - hypothetical protein, at 616,673 to 617,653 _0531 Position (kb) 615 616 617Strain fitness (log2 ratio) -2 -1 0 1 2at 615.730 kb on + strand, within PP_0530at 615.742 kb on + strand, within PP_0530at 615.742 kb on + strand, within PP_0530at 615.742 kb on + strand, within PP_0530at 615.742 kb on + strand, within PP_0530at 615.743 kb on - strand, within PP_0530at 615.744 kb on + strand, within PP_0530at 615.744 kb on + strand, within PP_0530at 615.744 kb on + strand, within PP_0530at 615.854 kb on + strand, within PP_0530at 615.924 kb on - strand, within PP_0530at 615.924 kb on - strand, within PP_0530at 616.001 kb on + strand, within PP_0530at 616.002 kb on - strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.163 kb on + strand, within PP_0530at 616.164 kb on - strand, within PP_0530at 616.164 kb on - strand, within PP_0530at 616.227 kb on + strand, within PP_0530at 616.228 kb on - strand, within PP_0530at 616.228 kb on - strand, within PP_0530at 616.405 kb on - strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.501 kb on + strandat 616.502 kb on - strandat 616.502 kb on - strandat 616.502 kb on - strandat 616.530 kb on + strandat 616.530 kb on + strandat 616.531 kb on - strandat 616.531 kb on - strandat 616.531 kb on - strandat 616.568 kb on - strandat 616.571 kb on - strandat 616.571 kb on - strandat 616.571 kb on - strandat 616.594 kb on + strandat 616.594 kb on + strandat 616.594 kb on + strandat 616.595 kb on - strandat 616.603 kb on - strandat 616.628 kb on + strandat 616.629 kb on - strandat 616.685 kb on + strandat 616.760 kb on + strandat 616.761 kb on - strandat 616.761 kb on - strandat 616.826 kb on + strand, within PP_0531at 616.826 kb on + strand, within PP_0531at 616.827 kb on - strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.871 kb on + strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.872 kb on - strand, within PP_0531at 616.884 kb on - strand, within PP_0531at 616.953 kb on - strand, within PP_0531at 616.953 kb on - strand, within PP_0531at 617.003 kb on + strand, within PP_0531at 617.003 kb on + strand, within PP_0531at 617.004 kb on - strand, within PP_0531at 617.004 kb on - strand, within PP_0531at 617.004 kb on - strand, within PP_0531at 617.004 kb on - strand, within PP_0531at 617.004 kb on - strand, within PP_0531at 617.021 kb on + strand, within PP_0531at 617.022 kb on - strand, within PP_0531at 617.273 kb on - strand, within PP_0531at 617.273 kb on - strand, within PP_0531at 617.280 kb on + strand, within PP_0531

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
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615,730 + PP_0530 0.12 +0.8
615,742 + PP_0530 0.14 -0.1
615,742 + PP_0530 0.14 -1.0
615,742 + PP_0530 0.14 -0.4
615,742 + PP_0530 0.14 -0.8
615,743 - PP_0530 0.14 +0.1
615,744 + PP_0530 0.14 +0.1
615,744 + PP_0530 0.14 +0.2
615,744 + PP_0530 0.14 +1.8
615,854 + PP_0530 0.31 -0.1
615,924 - PP_0530 0.42 -0.6
615,924 - PP_0530 0.42 -0.2
616,001 + PP_0530 0.54 -1.8
616,002 - PP_0530 0.54 +0.3
616,163 + PP_0530 0.79 -1.0
616,163 + PP_0530 0.79 -0.7
616,163 + PP_0530 0.79 -0.9
616,163 + PP_0530 0.79 +0.2
616,163 + PP_0530 0.79 -0.3
616,163 + PP_0530 0.79 -2.4
616,163 + PP_0530 0.79 +0.8
616,163 + PP_0530 0.79 -1.0
616,164 - PP_0530 0.79 -0.1
616,164 - PP_0530 0.79 -1.1
616,227 + PP_0530 0.88 +0.4
616,228 - PP_0530 0.89 -1.1
616,228 - PP_0530 0.89 -1.2
616,405 - -0.8
616,501 + -0.7
616,501 + +1.2
616,501 + -0.5
616,501 + -0.2
616,501 + +0.9
616,501 + +2.2
616,501 + +1.1
616,502 - -0.4
616,502 - +0.3
616,502 - -0.3
616,530 + -0.7
616,530 + -0.7
616,531 - -0.0
616,531 - -1.2
616,531 - -2.2
616,568 - -0.0
616,571 - -0.1
616,571 - -1.8
616,571 - -1.6
616,594 + -0.2
616,594 + +0.3
616,594 + -0.1
616,595 - -0.0
616,603 - +1.2
616,628 + +0.5
616,629 - -0.7
616,685 + -0.4
616,760 + +0.2
616,761 - +0.2
616,761 - -0.7
616,826 + PP_0531 0.16 +0.2
616,826 + PP_0531 0.16 -0.2
616,827 - PP_0531 0.16 -1.3
616,871 + PP_0531 0.20 -0.2
616,871 + PP_0531 0.20 +0.9
616,871 + PP_0531 0.20 -1.2
616,871 + PP_0531 0.20 +0.6
616,871 + PP_0531 0.20 +0.8
616,871 + PP_0531 0.20 -0.4
616,872 - PP_0531 0.20 +0.4
616,872 - PP_0531 0.20 +0.3
616,872 - PP_0531 0.20 -0.3
616,872 - PP_0531 0.20 -0.8
616,872 - PP_0531 0.20 +0.3
616,872 - PP_0531 0.20 +0.6
616,872 - PP_0531 0.20 -0.6
616,884 - PP_0531 0.22 +0.5
616,953 - PP_0531 0.29 -0.5
616,953 - PP_0531 0.29 -0.1
617,003 + PP_0531 0.34 +0.6
617,003 + PP_0531 0.34 -0.0
617,004 - PP_0531 0.34 -0.1
617,004 - PP_0531 0.34 -1.2
617,004 - PP_0531 0.34 +0.9
617,004 - PP_0531 0.34 +1.8
617,004 - PP_0531 0.34 +0.5
617,021 + PP_0531 0.35 -0.1
617,022 - PP_0531 0.36 -0.1
617,273 - PP_0531 0.61 -0.4
617,273 - PP_0531 0.61 +0.6
617,280 + PP_0531 0.62 +0.2

Or see this region's nucleotide sequence