Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0493 and PP_0494 overlap by 4 nucleotides PP_0494 and PP_t75 are separated by 6 nucleotides PP_t75 and PP_0495 are separated by 106 nucleotides
PP_0493: PP_0493 - selenocysteine synthase, at 580,711 to 582,138
_0493
PP_0494: PP_0494 - selenocysteyl-tRNA-specific translation elongation factor, at 582,135 to 584,057
_0494
PP_t75: PP_t75 - tRNA-Sec, at 584,064 to 584,159
_t75
PP_0495: PP_0495 - type 1 L-asparaginase, at 584,266 to 585,255
_0495
Position (kb) 582
583
584
585 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 581.193 kb on - strand, within PP_0493 at 581.193 kb on - strand, within PP_0493 at 581.331 kb on + strand, within PP_0493 at 581.332 kb on - strand, within PP_0493 at 581.332 kb on - strand, within PP_0493 at 581.347 kb on + strand, within PP_0493 at 581.404 kb on + strand, within PP_0493 at 581.404 kb on + strand, within PP_0493 at 581.405 kb on - strand, within PP_0493 at 581.405 kb on - strand, within PP_0493 at 581.436 kb on + strand, within PP_0493 at 581.437 kb on - strand, within PP_0493 at 581.454 kb on + strand, within PP_0493 at 581.454 kb on + strand, within PP_0493 at 581.455 kb on - strand, within PP_0493 at 581.474 kb on + strand, within PP_0493 at 581.475 kb on - strand, within PP_0493 at 581.517 kb on + strand, within PP_0493 at 581.549 kb on + strand, within PP_0493 at 582.329 kb on + strand, within PP_0494 at 582.380 kb on - strand, within PP_0494 at 582.650 kb on + strand, within PP_0494 at 582.650 kb on + strand, within PP_0494 at 582.651 kb on - strand, within PP_0494 at 582.651 kb on - strand, within PP_0494 at 582.651 kb on - strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.758 kb on + strand, within PP_0494 at 582.759 kb on - strand, within PP_0494 at 582.759 kb on - strand, within PP_0494 at 582.759 kb on - strand, within PP_0494 at 582.963 kb on + strand, within PP_0494 at 582.964 kb on - strand, within PP_0494 at 582.964 kb on - strand, within PP_0494 at 582.971 kb on - strand, within PP_0494 at 583.037 kb on - strand, within PP_0494 at 583.645 kb on + strand, within PP_0494 at 583.777 kb on + strand, within PP_0494 at 583.897 kb on + strand at 583.897 kb on + strand at 583.898 kb on - strand at 583.922 kb on + strand at 583.922 kb on + strand at 583.922 kb on + strand at 584.203 kb on - strand at 584.203 kb on - strand at 584.339 kb on - strand at 584.426 kb on + strand, within PP_0495 at 584.426 kb on + strand, within PP_0495 at 584.427 kb on - strand, within PP_0495 at 584.427 kb on - strand, within PP_0495 at 584.427 kb on - strand, within PP_0495 at 584.427 kb on - strand, within PP_0495 at 584.467 kb on - strand, within PP_0495 at 584.552 kb on + strand, within PP_0495 at 584.586 kb on - strand, within PP_0495 at 584.587 kb on + strand, within PP_0495 at 584.626 kb on + strand, within PP_0495 at 584.670 kb on - strand, within PP_0495 at 584.670 kb on - strand, within PP_0495 at 584.756 kb on - strand, within PP_0495 at 584.798 kb on + strand, within PP_0495 at 584.798 kb on + strand, within PP_0495 at 584.798 kb on + strand, within PP_0495 at 584.799 kb on - strand, within PP_0495 at 584.911 kb on + strand, within PP_0495 at 584.912 kb on - strand, within PP_0495 at 584.912 kb on - strand, within PP_0495 at 584.912 kb on - strand, within PP_0495 at 584.961 kb on - strand, within PP_0495
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 581,193 - PP_0493 0.34 -0.0 581,193 - PP_0493 0.34 +0.5 581,331 + PP_0493 0.43 -1.3 581,332 - PP_0493 0.43 -0.0 581,332 - PP_0493 0.43 +1.4 581,347 + PP_0493 0.45 +0.4 581,404 + PP_0493 0.49 -1.4 581,404 + PP_0493 0.49 +0.1 581,405 - PP_0493 0.49 -0.6 581,405 - PP_0493 0.49 -0.2 581,436 + PP_0493 0.51 +1.2 581,437 - PP_0493 0.51 +1.5 581,454 + PP_0493 0.52 +0.9 581,454 + PP_0493 0.52 -2.9 581,455 - PP_0493 0.52 +2.8 581,474 + PP_0493 0.53 +1.2 581,475 - PP_0493 0.54 -1.4 581,517 + PP_0493 0.56 +0.5 581,549 + PP_0493 0.59 -2.7 582,329 + PP_0494 0.10 +0.5 582,380 - PP_0494 0.13 -1.0 582,650 + PP_0494 0.27 +0.6 582,650 + PP_0494 0.27 +0.3 582,651 - PP_0494 0.27 +1.0 582,651 - PP_0494 0.27 +0.5 582,651 - PP_0494 0.27 +2.8 582,758 + PP_0494 0.32 +0.9 582,758 + PP_0494 0.32 -0.1 582,758 + PP_0494 0.32 +1.4 582,758 + PP_0494 0.32 +1.6 582,758 + PP_0494 0.32 -0.6 582,758 + PP_0494 0.32 -0.1 582,759 - PP_0494 0.32 +0.5 582,759 - PP_0494 0.32 +0.3 582,759 - PP_0494 0.32 +0.3 582,963 + PP_0494 0.43 +0.9 582,964 - PP_0494 0.43 +0.5 582,964 - PP_0494 0.43 +0.1 582,971 - PP_0494 0.43 +0.3 583,037 - PP_0494 0.47 +0.7 583,645 + PP_0494 0.79 -0.2 583,777 + PP_0494 0.85 +0.7 583,897 + +0.9 583,897 + +0.2 583,898 - -1.8 583,922 + +0.2 583,922 + +1.5 583,922 + -0.4 584,203 - +1.1 584,203 - +0.2 584,339 - +0.0 584,426 + PP_0495 0.16 +1.5 584,426 + PP_0495 0.16 -0.5 584,427 - PP_0495 0.16 +2.1 584,427 - PP_0495 0.16 -0.0 584,427 - PP_0495 0.16 +0.4 584,427 - PP_0495 0.16 +1.4 584,467 - PP_0495 0.20 +2.2 584,552 + PP_0495 0.29 -1.1 584,586 - PP_0495 0.32 -0.0 584,587 + PP_0495 0.32 +0.7 584,626 + PP_0495 0.36 +0.8 584,670 - PP_0495 0.41 +1.6 584,670 - PP_0495 0.41 +0.5 584,756 - PP_0495 0.49 -1.4 584,798 + PP_0495 0.54 +1.1 584,798 + PP_0495 0.54 +1.2 584,798 + PP_0495 0.54 +2.8 584,799 - PP_0495 0.54 +0.8 584,911 + PP_0495 0.65 +3.2 584,912 - PP_0495 0.65 +2.8 584,912 - PP_0495 0.65 +0.8 584,912 - PP_0495 0.65 -0.8 584,961 - PP_0495 0.70 -0.0
Or see this region's nucleotide sequence