Strain Fitness in Pseudomonas putida KT2440 around PP_0260

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
313,723	+		PP_0258	0.76	-0.9
313,724	-		PP_0258	0.76	-1.5
313,741	+		PP_0258	0.80	+0.3
313,742	-		PP_0258	0.80	+0.4
313,841	+				+0.9
313,841	+				-0.7
313,879	+				-1.3
313,880	-				+0.1
313,887	+				-1.0
313,893	+				-2.0
314,110	+		PP_0259	0.35	+0.7
314,110	+		PP_0259	0.35	+0.3
314,111	-		PP_0259	0.35	-0.2
314,140	+		PP_0259	0.40	-0.4
314,140	+		PP_0259	0.40	+2.7
314,140	+		PP_0259	0.40	-0.9
314,140	+		PP_0259	0.40	+2.7
314,141	-		PP_0259	0.40	+1.1
314,141	-		PP_0259	0.40	-1.4
314,141	-		PP_0259	0.40	-0.4
314,247	-		PP_0259	0.56	-0.5
314,313	+		PP_0259	0.66	+0.2
314,313	+		PP_0259	0.66	+0.2
314,314	-		PP_0259	0.66	+2.0
314,314	-		PP_0259	0.66	-0.5
314,341	+		PP_0259	0.70	-1.4
314,410	-		PP_0259	0.80	-0.3
314,467	+		PP_0259	0.89	+0.1
314,468	-		PP_0259	0.89	-0.8
314,468	-		PP_0259	0.89	+0.3
314,572	+				-0.7
314,572	+				+0.4
314,572	+				+0.7
314,572	+				+0.7
314,573	-				-0.1
314,573	-				-1.3
314,574	+				+0.8
314,574	+				-0.8
314,574	+				+0.5
314,574	+				-2.0
314,574	+				+2.7
314,575	-				-0.2
314,575	-				-0.5
314,575	-				+1.7
314,575	-				+0.0
314,575	-				-0.4
314,575	-				-0.5
314,575	-				-1.0
314,577	+				-0.2
314,624	+				+0.6
314,675	+		PP_0260	0.12	+2.7
314,675	+		PP_0260	0.12	-0.5
314,710	+		PP_0260	0.19	+0.5
314,710	+		PP_0260	0.19	+1.7
314,710	+		PP_0260	0.19	-0.2
314,710	+		PP_0260	0.19	-1.9
314,711	-		PP_0260	0.19	-3.0
314,711	-		PP_0260	0.19	-1.0
314,711	-		PP_0260	0.19	-2.8
314,711	-		PP_0260	0.19	-1.5
314,800	+		PP_0260	0.34	+0.7
314,801	-		PP_0260	0.35	-1.7
314,821	+		PP_0260	0.38	-0.9
314,822	-		PP_0260	0.38	-0.7
314,822	-		PP_0260	0.38	-2.6
314,959	+		PP_0260	0.62	+0.7
314,960	-		PP_0260	0.63	-2.7
314,960	-		PP_0260	0.63	-0.3
314,960	-		PP_0260	0.63	-0.6
314,960	-		PP_0260	0.63	-0.5
315,167	+				+1.7
315,167	+				+0.5
315,167	+				+0.7
315,167	+				-0.9
315,167	+				-0.6
315,167	+				-0.4
315,167	+				-0.0
315,168	-				-1.6
315,301	+		PP_0261	0.14	-2.5
315,380	+		PP_0261	0.24	-2.0
315,380	+		PP_0261	0.24	-0.5
315,380	+		PP_0261	0.24	-0.7
315,381	-		PP_0261	0.24	-2.3
315,381	-		PP_0261	0.24	-0.9
315,381	-		PP_0261	0.24	-0.9
315,381	-		PP_0261	0.24	-2.3
315,550	-		PP_0261	0.45	-1.5
315,616	+		PP_0261	0.54	-1.6
315,616	+		PP_0261	0.54	-1.1
315,616	+		PP_0261	0.54	-3.4
315,617	-		PP_0261	0.54	-2.1
315,617	-		PP_0261	0.54	-1.8
315,825	-		PP_0261	0.80	-2.2
315,920	+				-2.1
315,927	+				-2.7
315,927	+				+2.7
315,927	+				-2.5
315,946	+				-2.8
315,984	+				-0.3
315,984	+				+2.4
315,984	+				-0.3
315,984	+				+0.5
315,984	+				-0.5
315,984	+				-1.3
315,984	+				-2.0
315,984	+				-3.4
315,985	-				-0.9
316,067	+				+1.1
316,067	+				-1.9
316,067	+				+0.3
316,067	+				-1.2
316,068	-				-0.8
316,070	+				+1.3
316,070	+				+0.4
316,071	-				-0.2
316,071	-				-1.5
316,085	+				-0.9
316,085	+				-0.2
316,086	-				+1.0