Strain Fitness in Pseudomonas putida KT2440 around PP_0200

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0198 and PP_0199 are separated by 48 nucleotidesPP_0199 and PP_0200 are separated by 8 nucleotidesPP_0200 and PP_0201 are separated by 65 nucleotidesPP_0201 and PP_5432 are separated by 61 nucleotidesPP_5432 and PP_5433 are separated by 69 nucleotides PP_0198: PP_0198 - putative transporter, at 248,420 to 249,052 _0198 PP_0199: PP_0199 - putative membrane protease family, stomatin, at 249,101 to 249,847 _0199 PP_0200: PP_0200 - putative membrane bound peptidase, nefD, at 249,856 to 251,202 _0200 PP_0201: PP_0201 - conserved protein of unknown function, at 251,268 to 251,759 _0201 PP_5432: PP_5432 - conserved protein of unknown function, at 251,821 to 252,063 _5432 PP_5433: PP_5433 - putative NADH:ubiquinone oxidoreductase subunit 2, at 252,133 to 252,771 _5433 Position (kb) 249 250 251 252Strain fitness (log2 ratio) -2 -1 0 1 2at 248.864 kb on + strand, within PP_0198at 248.864 kb on + strand, within PP_0198at 248.865 kb on - strand, within PP_0198at 248.865 kb on - strand, within PP_0198at 248.919 kb on - strand, within PP_0198at 249.062 kb on + strandat 249.063 kb on - strandat 249.102 kb on + strandat 249.102 kb on + strandat 249.103 kb on - strandat 249.137 kb on + strandat 249.199 kb on + strand, within PP_0199at 249.199 kb on + strandat 249.199 kb on + strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.200 kb on - strand, within PP_0199at 249.201 kb on + strand, within PP_0199at 249.201 kb on + strandat 249.202 kb on - strand, within PP_0199at 249.202 kb on - strand, within PP_0199at 249.202 kb on - strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.425 kb on + strand, within PP_0199at 249.647 kb on + strand, within PP_0199at 249.664 kb on + strand, within PP_0199at 249.664 kb on + strand, within PP_0199at 249.664 kb on + strandat 249.664 kb on + strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.665 kb on - strand, within PP_0199at 249.680 kb on + strand, within PP_0199at 249.681 kb on - strand, within PP_0199at 249.772 kb on - strand, within PP_0199at 249.925 kb on + strandat 249.925 kb on + strandat 250.341 kb on - strand, within PP_0200at 250.341 kb on - strand, within PP_0200at 250.341 kb on - strand, within PP_0200at 250.362 kb on + strand, within PP_0200at 250.362 kb on + strandat 250.363 kb on - strand, within PP_0200at 250.363 kb on - strand, within PP_0200at 250.451 kb on - strand, within PP_0200at 250.724 kb on + strand, within PP_0200at 250.785 kb on + strand, within PP_0200at 250.870 kb on - strand, within PP_0200at 250.870 kb on - strand, within PP_0200at 250.870 kb on - strand, within PP_0200at 250.887 kb on + strand, within PP_0200at 250.887 kb on + strand, within PP_0200at 250.888 kb on - strand, within PP_0200at 250.888 kb on - strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.889 kb on + strand, within PP_0200at 250.890 kb on - strand, within PP_0200at 250.890 kb on - strand, within PP_0200at 250.890 kb on - strand, within PP_0200at 250.931 kb on + strand, within PP_0200at 250.931 kb on + strand, within PP_0200at 250.932 kb on - strand, within PP_0200at 250.932 kb on - strand, within PP_0200at 251.073 kb on - strandat 251.226 kb on - strandat 251.229 kb on - strandat 251.352 kb on + strand, within PP_0201at 251.352 kb on + strand, within PP_0201at 251.353 kb on - strand, within PP_0201at 251.353 kb on - strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.466 kb on + strand, within PP_0201at 251.467 kb on - strand, within PP_0201at 251.467 kb on - strand, within PP_0201at 251.467 kb on - strand, within PP_0201at 251.467 kb on - strand, within PP_0201at 251.614 kb on - strand, within PP_0201at 251.614 kb on - strand, within PP_0201at 251.664 kb on + strand, within PP_0201at 251.665 kb on - strand, within PP_0201at 251.715 kb on + strandat 251.715 kb on + strandat 251.716 kb on - strandat 251.716 kb on - strandat 251.716 kb on - strandat 251.716 kb on - strandat 251.889 kb on + strand, within PP_5432at 252.101 kb on + strandat 252.101 kb on + strandat 252.101 kb on + strandat 252.102 kb on - strandat 252.102 kb on - strandat 252.102 kb on - strandat 252.102 kb on - strandat 252.119 kb on - strandat 252.199 kb on + strand, within PP_5433at 252.199 kb on + strand, within PP_5433at 252.200 kb on - strand, within PP_5433

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
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248,864 + PP_0198 0.70 +1.7
248,864 + PP_0198 0.70 +0.9
248,865 - PP_0198 0.70 +1.4
248,865 - PP_0198 0.70 +1.1
248,919 - PP_0198 0.79 +2.7
249,062 + +0.3
249,063 - +2.7
249,102 + +0.1
249,102 + -2.2
249,103 - +0.2
249,137 + +0.2
249,199 + PP_0199 0.13 +1.0
249,199 + +1.4
249,199 + PP_0199 0.13 +0.5
249,200 - PP_0199 0.13 +1.4
249,200 - PP_0199 0.13 +1.3
249,200 - PP_0199 0.13 -0.1
249,201 + PP_0199 0.13 +0.5
249,201 + +1.7
249,202 - PP_0199 0.14 -0.3
249,202 - PP_0199 0.14 +0.0
249,202 - PP_0199 0.14 -1.1
249,425 + PP_0199 0.43 +0.7
249,425 + PP_0199 0.43 +2.7
249,425 + PP_0199 0.43 +0.8
249,647 + PP_0199 0.73 -0.1
249,664 + PP_0199 0.75 +2.7
249,664 + PP_0199 0.75 -0.5
249,664 + +0.7
249,664 + PP_0199 0.75 +0.4
249,665 - PP_0199 0.76 +1.2
249,665 - PP_0199 0.76 -1.4
249,665 - PP_0199 0.76 -1.3
249,680 + PP_0199 0.78 -0.2
249,681 - PP_0199 0.78 -0.8
249,772 - PP_0199 0.90 +0.1
249,925 + -0.3
249,925 + -0.1
250,341 - PP_0200 0.36 -0.9
250,341 - PP_0200 0.36 +0.3
250,341 - PP_0200 0.36 +0.7
250,362 + PP_0200 0.38 -0.5
250,362 + +1.1
250,363 - PP_0200 0.38 -1.2
250,363 - PP_0200 0.38 +0.0
250,451 - PP_0200 0.44 -1.1
250,724 + PP_0200 0.64 -1.9
250,785 + PP_0200 0.69 -0.4
250,870 - PP_0200 0.75 +0.1
250,870 - PP_0200 0.75 +0.6
250,870 - PP_0200 0.75 -0.1
250,887 + PP_0200 0.77 -1.3
250,887 + PP_0200 0.77 +0.2
250,888 - PP_0200 0.77 -1.5
250,888 - PP_0200 0.77 +0.7
250,889 + PP_0200 0.77 -0.7
250,889 + PP_0200 0.77 -0.4
250,889 + PP_0200 0.77 +0.1
250,889 + PP_0200 0.77 +0.1
250,889 + PP_0200 0.77 +1.0
250,889 + PP_0200 0.77 +0.8
250,889 + PP_0200 0.77 +1.7
250,889 + PP_0200 0.77 -0.1
250,889 + PP_0200 0.77 +0.2
250,890 - PP_0200 0.77 +0.4
250,890 - PP_0200 0.77 +1.0
250,890 - PP_0200 0.77 -0.2
250,931 + PP_0200 0.80 +1.7
250,931 + PP_0200 0.80 +0.5
250,932 - PP_0200 0.80 +0.3
250,932 - PP_0200 0.80 -0.0
251,073 - +0.0
251,226 - -0.3
251,229 - +0.5
251,352 + PP_0201 0.17 +0.5
251,352 + PP_0201 0.17 -0.8
251,353 - PP_0201 0.17 +1.9
251,353 - PP_0201 0.17 +1.0
251,466 + PP_0201 0.40 -0.9
251,466 + PP_0201 0.40 +0.4
251,466 + PP_0201 0.40 -0.2
251,466 + PP_0201 0.40 -0.1
251,466 + PP_0201 0.40 -1.5
251,466 + PP_0201 0.40 -0.8
251,467 - PP_0201 0.40 -0.3
251,467 - PP_0201 0.40 -0.3
251,467 - PP_0201 0.40 -0.3
251,467 - PP_0201 0.40 +0.2
251,614 - PP_0201 0.70 +0.3
251,614 - PP_0201 0.70 -0.9
251,664 + PP_0201 0.80 +0.3
251,665 - PP_0201 0.81 +0.5
251,715 + +0.3
251,715 + -1.7
251,716 - +0.4
251,716 - +0.0
251,716 - +2.7
251,716 - -0.7
251,889 + PP_5432 0.28 +0.7
252,101 + -0.8
252,101 + -0.2
252,101 + -1.0
252,102 - -1.6
252,102 - +1.7
252,102 - +0.3
252,102 - +0.3
252,119 - -0.4
252,199 + PP_5433 0.10 -0.8
252,199 + PP_5433 0.10 -0.2
252,200 - PP_5433 0.10 -0.3

Or see this region's nucleotide sequence