Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0196 and PP_0197 overlap by 1 nucleotides PP_0197 and PP_0198 are separated by 99 nucleotides PP_0198 and PP_0199 are separated by 48 nucleotides
PP_0196: PP_0196 - putative of ABC superfamily informational factor: ATP-binding components, at 245,823 to 247,733
_0196
PP_0197: PP_0197 - conserved protein of unknown function, at 247,733 to 248,320
_0197
PP_0198: PP_0198 - putative transporter, at 248,420 to 249,052
_0198
PP_0199: PP_0199 - putative membrane protease family, stomatin, at 249,101 to 249,847
_0199
Position (kb) 247
248
249 Strain fitness (log2 ratio) -2
-1
0
1
2 at 246.842 kb on + strand, within PP_0196 at 246.842 kb on + strand, within PP_0196 at 246.952 kb on + strand, within PP_0196 at 246.957 kb on + strand, within PP_0196 at 246.957 kb on + strand, within PP_0196 at 246.958 kb on - strand, within PP_0196 at 246.958 kb on - strand, within PP_0196 at 246.958 kb on - strand, within PP_0196 at 246.958 kb on - strand, within PP_0196 at 246.958 kb on - strand, within PP_0196 at 247.365 kb on + strand, within PP_0196 at 247.366 kb on - strand, within PP_0196 at 247.366 kb on - strand, within PP_0196 at 247.366 kb on - strand, within PP_0196 at 247.412 kb on + strand, within PP_0196 at 247.587 kb on + strand at 247.835 kb on - strand, within PP_0197 at 248.165 kb on - strand, within PP_0197 at 248.233 kb on + strand, within PP_0197 at 248.233 kb on + strand, within PP_0197 at 248.233 kb on + strand, within PP_0197 at 248.233 kb on + strand, within PP_0197 at 248.234 kb on - strand, within PP_0197 at 248.234 kb on - strand, within PP_0197 at 248.281 kb on - strand at 248.380 kb on - strand at 248.433 kb on + strand at 248.433 kb on + strand at 248.433 kb on + strand at 248.433 kb on + strand at 248.433 kb on + strand at 248.434 kb on - strand at 248.434 kb on - strand at 248.434 kb on - strand at 248.434 kb on - strand at 248.672 kb on + strand, within PP_0198 at 248.681 kb on + strand, within PP_0198 at 248.681 kb on + strand, within PP_0198 at 248.682 kb on - strand, within PP_0198 at 248.864 kb on + strand, within PP_0198 at 248.864 kb on + strand, within PP_0198 at 248.865 kb on - strand, within PP_0198 at 248.865 kb on - strand, within PP_0198 at 248.919 kb on - strand, within PP_0198 at 249.062 kb on + strand at 249.063 kb on - strand at 249.102 kb on + strand at 249.102 kb on + strand at 249.103 kb on - strand at 249.137 kb on + strand at 249.199 kb on + strand, within PP_0199 at 249.199 kb on + strand at 249.199 kb on + strand, within PP_0199 at 249.200 kb on - strand, within PP_0199 at 249.200 kb on - strand, within PP_0199 at 249.200 kb on - strand, within PP_0199 at 249.201 kb on + strand, within PP_0199 at 249.201 kb on + strand at 249.202 kb on - strand, within PP_0199 at 249.202 kb on - strand, within PP_0199 at 249.202 kb on - strand, within PP_0199
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 246,842 + PP_0196 0.53 +0.6 246,842 + PP_0196 0.53 +2.7 246,952 + PP_0196 0.59 +0.1 246,957 + PP_0196 0.59 +0.2 246,957 + PP_0196 0.59 +2.7 246,958 - PP_0196 0.59 +2.7 246,958 - PP_0196 0.59 -0.1 246,958 - PP_0196 0.59 -0.6 246,958 - PP_0196 0.59 -0.7 246,958 - PP_0196 0.59 +0.4 247,365 + PP_0196 0.81 -0.1 247,366 - PP_0196 0.81 -0.6 247,366 - PP_0196 0.81 +1.7 247,366 - PP_0196 0.81 +1.0 247,412 + PP_0196 0.83 -0.3 247,587 + -0.9 247,835 - PP_0197 0.17 +0.4 248,165 - PP_0197 0.73 +0.1 248,233 + PP_0197 0.85 -0.2 248,233 + PP_0197 0.85 +1.0 248,233 + PP_0197 0.85 -0.6 248,233 + PP_0197 0.85 +0.5 248,234 - PP_0197 0.85 +0.6 248,234 - PP_0197 0.85 -0.3 248,281 - -1.7 248,380 - -0.2 248,433 + -0.2 248,433 + +0.2 248,433 + +2.7 248,433 + +1.8 248,433 + +0.8 248,434 - +0.2 248,434 - +0.1 248,434 - +2.7 248,434 - +1.1 248,672 + PP_0198 0.40 -0.3 248,681 + PP_0198 0.41 +0.1 248,681 + PP_0198 0.41 +2.1 248,682 - PP_0198 0.41 -1.1 248,864 + PP_0198 0.70 +1.7 248,864 + PP_0198 0.70 +0.9 248,865 - PP_0198 0.70 +1.4 248,865 - PP_0198 0.70 +1.1 248,919 - PP_0198 0.79 +2.7 249,062 + +0.3 249,063 - +2.7 249,102 + +0.1 249,102 + -2.2 249,103 - +0.2 249,137 + +0.2 249,199 + PP_0199 0.13 +1.0 249,199 + +1.4 249,199 + PP_0199 0.13 +0.5 249,200 - PP_0199 0.13 +1.4 249,200 - PP_0199 0.13 +1.3 249,200 - PP_0199 0.13 -0.1 249,201 + PP_0199 0.13 +0.5 249,201 + +1.7 249,202 - PP_0199 0.14 -0.3 249,202 - PP_0199 0.14 +0.0 249,202 - PP_0199 0.14 -1.1
Or see this region's nucleotide sequence