Strain Fitness in Pseudomonas putida KT2440 around PP_0196

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0194 and PP_0195 overlap by 22 nucleotidesPP_0195 and PP_0196 are separated by 50 nucleotidesPP_0196 and PP_0197 overlap by 1 nucleotidesPP_0197 and PP_0198 are separated by 99 nucleotides PP_0194: PP_0194 - Transcriptional regulatory protein AlgP, at 244,325 to 245,314 _0194 PP_0195: PP_0195 - conserved protein of unknown function, at 245,293 to 245,772 _0195 PP_0196: PP_0196 - putative of ABC superfamily informational factor: ATP-binding components, at 245,823 to 247,733 _0196 PP_0197: PP_0197 - conserved protein of unknown function, at 247,733 to 248,320 _0197 PP_0198: PP_0198 - putative transporter, at 248,420 to 249,052 _0198 Position (kb) 245 246 247 248Strain fitness (log2 ratio) -2 -1 0 1 2at 245.053 kb on + strand, within PP_0194at 245.629 kb on + strand, within PP_0195at 245.630 kb on - strand, within PP_0195at 245.735 kb on + strandat 245.735 kb on + strandat 245.735 kb on + strandat 245.736 kb on - strandat 245.807 kb on - strandat 245.848 kb on + strandat 245.849 kb on - strandat 245.849 kb on - strandat 245.872 kb on + strandat 245.872 kb on + strandat 245.872 kb on + strandat 245.872 kb on + strandat 245.873 kb on - strandat 245.873 kb on - strandat 246.069 kb on + strand, within PP_0196at 246.069 kb on + strand, within PP_0196at 246.069 kb on + strand, within PP_0196at 246.069 kb on + strand, within PP_0196at 246.069 kb on + strand, within PP_0196at 246.070 kb on - strand, within PP_0196at 246.070 kb on - strand, within PP_0196at 246.190 kb on - strand, within PP_0196at 246.190 kb on - strand, within PP_0196at 246.190 kb on - strandat 246.192 kb on - strand, within PP_0196at 246.502 kb on - strand, within PP_0196at 246.567 kb on + strand, within PP_0196at 246.567 kb on + strand, within PP_0196at 246.607 kb on - strand, within PP_0196at 246.607 kb on - strand, within PP_0196at 246.842 kb on + strand, within PP_0196at 246.842 kb on + strand, within PP_0196at 246.952 kb on + strand, within PP_0196at 246.957 kb on + strand, within PP_0196at 246.957 kb on + strand, within PP_0196at 246.958 kb on - strand, within PP_0196at 246.958 kb on - strand, within PP_0196at 246.958 kb on - strand, within PP_0196at 246.958 kb on - strand, within PP_0196at 246.958 kb on - strand, within PP_0196at 247.365 kb on + strand, within PP_0196at 247.366 kb on - strand, within PP_0196at 247.366 kb on - strand, within PP_0196at 247.366 kb on - strand, within PP_0196at 247.412 kb on + strand, within PP_0196at 247.587 kb on + strandat 247.835 kb on - strand, within PP_0197at 248.165 kb on - strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.233 kb on + strand, within PP_0197at 248.234 kb on - strand, within PP_0197at 248.234 kb on - strand, within PP_0197at 248.281 kb on - strandat 248.380 kb on - strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.433 kb on + strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.434 kb on - strandat 248.672 kb on + strand, within PP_0198at 248.681 kb on + strand, within PP_0198at 248.681 kb on + strand, within PP_0198at 248.682 kb on - strand, within PP_0198

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
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245,053 + PP_0194 0.74 +0.4
245,629 + PP_0195 0.70 +0.3
245,630 - PP_0195 0.70 +0.2
245,735 + +1.9
245,735 + +0.7
245,735 + -0.5
245,736 - -0.7
245,807 - +0.2
245,848 + -0.7
245,849 - -1.8
245,849 - -0.1
245,872 + -1.2
245,872 + +1.1
245,872 + +1.7
245,872 + +0.5
245,873 - +0.4
245,873 - +2.7
246,069 + PP_0196 0.13 +0.7
246,069 + PP_0196 0.13 +0.2
246,069 + PP_0196 0.13 +0.6
246,069 + PP_0196 0.13 -1.2
246,069 + PP_0196 0.13 +0.7
246,070 - PP_0196 0.13 +0.2
246,070 - PP_0196 0.13 +0.2
246,190 - PP_0196 0.19 +0.9
246,190 - PP_0196 0.19 +0.9
246,190 - +2.7
246,192 - PP_0196 0.19 +1.4
246,502 - PP_0196 0.36 -0.2
246,567 + PP_0196 0.39 -0.6
246,567 + PP_0196 0.39 +0.1
246,607 - PP_0196 0.41 -0.5
246,607 - PP_0196 0.41 +0.2
246,842 + PP_0196 0.53 +0.6
246,842 + PP_0196 0.53 +2.7
246,952 + PP_0196 0.59 +0.1
246,957 + PP_0196 0.59 +0.2
246,957 + PP_0196 0.59 +2.7
246,958 - PP_0196 0.59 +2.7
246,958 - PP_0196 0.59 -0.1
246,958 - PP_0196 0.59 -0.6
246,958 - PP_0196 0.59 -0.7
246,958 - PP_0196 0.59 +0.4
247,365 + PP_0196 0.81 -0.1
247,366 - PP_0196 0.81 -0.6
247,366 - PP_0196 0.81 +1.7
247,366 - PP_0196 0.81 +1.0
247,412 + PP_0196 0.83 -0.3
247,587 + -0.9
247,835 - PP_0197 0.17 +0.4
248,165 - PP_0197 0.73 +0.1
248,233 + PP_0197 0.85 -0.2
248,233 + PP_0197 0.85 +1.0
248,233 + PP_0197 0.85 -0.6
248,233 + PP_0197 0.85 +0.5
248,234 - PP_0197 0.85 +0.6
248,234 - PP_0197 0.85 -0.3
248,281 - -1.7
248,380 - -0.2
248,433 + -0.2
248,433 + +0.2
248,433 + +2.7
248,433 + +1.8
248,433 + +0.8
248,434 - +0.2
248,434 - +0.1
248,434 - +2.7
248,434 - +1.1
248,672 + PP_0198 0.40 -0.3
248,681 + PP_0198 0.41 +0.1
248,681 + PP_0198 0.41 +2.1
248,682 - PP_0198 0.41 -1.1

Or see this region's nucleotide sequence