Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0006 and PP_0007 are separated by 2 nucleotides PP_0007 and PP_0008 overlap by 8 nucleotides PP_0008 and PP_0009 are separated by 13 nucleotides
PP_0006: PP_0006 - probable membrane protein insertase, at 6,471 to 8,153
_0006
PP_0007: PP_0007 - conserved hypothetical protein, at 8,156 to 8,401
_0007
PP_0008: PP_0008 - ribonuclease P protein component, at 8,394 to 8,798
_0008
PP_0009: PP_0009 - 50S ribosomal protein L34, at 8,812 to 8,946
_0009
Position (kb)
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 8.209 kb on - strand, within PP_0007 at 8.209 kb on - strand, within PP_0007 at 8.299 kb on - strand at 8.299 kb on - strand, within PP_0007 at 8.354 kb on - strand, within PP_0007 at 8.396 kb on - strand at 9.056 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.058 kb on - strand at 9.152 kb on + strand at 9.154 kb on + strand at 9.154 kb on + strand at 9.155 kb on - strand at 9.177 kb on - strand at 9.186 kb on - strand at 9.238 kb on + strand at 9.238 kb on + strand at 9.249 kb on - strand at 9.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 8,209 - PP_0007 0.22 -0.9 8,209 - PP_0007 0.22 -2.0 8,299 - +2.7 8,299 - PP_0007 0.58 -0.6 8,354 - PP_0007 0.80 +0.7 8,396 - +2.7 9,056 - +1.3 9,058 - -0.9 9,058 - +0.4 9,058 - +0.6 9,058 - +0.4 9,152 + +2.7 9,154 + +2.7 9,154 + +2.7 9,155 - +1.3 9,177 - +0.2 9,186 - +1.7 9,238 + +1.5 9,238 + -0.1 9,249 - -0.6 9,395 - -0.5
Or see this region's nucleotide sequence