Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5458 and Atu5459 are separated by 24 nucleotides Atu5459 and Atu5460 are separated by 83 nucleotides
Atu5458: Atu5458 - isomerase/lactonizing enzyme, at 450,316 to 451,482
Atu5458
Atu5459: Atu5459 - ABC transporter substrate binding protein (sugar), at 451,507 to 452,778
Atu5459
Atu5460: Atu5460 - ABC transporter membrane spanning protein, at 452,862 to 453,788
Atu5460
Position (kb)
451
452
453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 450.513 kb on + strand, within Atu5458 at 450.514 kb on + strand, within Atu5458 at 450.558 kb on + strand, within Atu5458 at 450.566 kb on - strand, within Atu5458 at 450.605 kb on + strand, within Atu5458 at 450.651 kb on + strand, within Atu5458 at 450.659 kb on - strand, within Atu5458 at 450.713 kb on + strand, within Atu5458 at 450.772 kb on + strand, within Atu5458 at 450.778 kb on - strand, within Atu5458 at 450.793 kb on - strand, within Atu5458 at 450.803 kb on - strand, within Atu5458 at 450.851 kb on + strand, within Atu5458 at 450.955 kb on + strand, within Atu5458 at 450.963 kb on - strand, within Atu5458 at 451.006 kb on + strand, within Atu5458 at 451.021 kb on - strand, within Atu5458 at 451.021 kb on - strand, within Atu5458 at 451.041 kb on + strand, within Atu5458 at 451.188 kb on + strand, within Atu5458 at 451.194 kb on - strand, within Atu5458 at 451.255 kb on - strand, within Atu5458 at 451.285 kb on - strand, within Atu5458 at 451.329 kb on - strand, within Atu5458 at 451.410 kb on - strand at 451.444 kb on - strand at 451.444 kb on - strand at 451.452 kb on + strand at 451.531 kb on + strand at 451.587 kb on + strand at 451.595 kb on - strand at 451.637 kb on - strand, within Atu5459 at 451.798 kb on + strand, within Atu5459 at 451.823 kb on + strand, within Atu5459 at 451.826 kb on - strand, within Atu5459 at 451.831 kb on - strand, within Atu5459 at 451.905 kb on + strand, within Atu5459 at 451.906 kb on + strand, within Atu5459 at 451.907 kb on + strand, within Atu5459 at 451.913 kb on - strand, within Atu5459 at 451.951 kb on + strand, within Atu5459 at 452.022 kb on + strand, within Atu5459 at 452.070 kb on + strand, within Atu5459 at 452.070 kb on + strand, within Atu5459 at 452.089 kb on + strand, within Atu5459 at 452.159 kb on - strand, within Atu5459 at 452.200 kb on + strand, within Atu5459 at 452.208 kb on - strand, within Atu5459 at 452.217 kb on + strand, within Atu5459 at 452.259 kb on - strand, within Atu5459 at 452.318 kb on + strand, within Atu5459 at 452.326 kb on - strand, within Atu5459 at 452.327 kb on - strand, within Atu5459 at 452.367 kb on + strand, within Atu5459 at 452.593 kb on - strand, within Atu5459 at 452.744 kb on - strand at 452.751 kb on + strand at 452.772 kb on + strand at 452.788 kb on + strand at 452.807 kb on + strand at 452.824 kb on + strand at 452.830 kb on - strand at 452.935 kb on + strand at 452.935 kb on + strand at 452.943 kb on - strand at 452.966 kb on - strand, within Atu5460 at 452.966 kb on - strand, within Atu5460 at 452.966 kb on - strand, within Atu5460 at 453.031 kb on - strand, within Atu5460 at 453.071 kb on + strand, within Atu5460 at 453.073 kb on + strand, within Atu5460 at 453.082 kb on - strand, within Atu5460 at 453.118 kb on + strand, within Atu5460 at 453.124 kb on + strand, within Atu5460 at 453.124 kb on + strand, within Atu5460 at 453.134 kb on + strand, within Atu5460 at 453.134 kb on + strand, within Atu5460 at 453.163 kb on + strand, within Atu5460 at 453.232 kb on - strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.239 kb on + strand, within Atu5460 at 453.242 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.247 kb on - strand, within Atu5460 at 453.269 kb on + strand, within Atu5460 at 453.354 kb on - strand, within Atu5460 at 453.412 kb on - strand, within Atu5460 at 453.463 kb on - strand, within Atu5460 at 453.510 kb on - strand, within Atu5460 at 453.564 kb on - strand, within Atu5460 at 453.578 kb on + strand, within Atu5460 at 453.578 kb on + strand, within Atu5460 at 453.586 kb on - strand, within Atu5460 at 453.642 kb on + strand, within Atu5460 at 453.672 kb on - strand, within Atu5460 at 453.717 kb on + strand at 453.726 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 450,513 + Atu5458 0.17 +1.3 450,514 + Atu5458 0.17 +0.9 450,558 + Atu5458 0.21 -1.6 450,566 - Atu5458 0.21 +0.0 450,605 + Atu5458 0.25 +2.6 450,651 + Atu5458 0.29 +0.0 450,659 - Atu5458 0.29 -1.0 450,713 + Atu5458 0.34 +0.5 450,772 + Atu5458 0.39 -0.6 450,778 - Atu5458 0.40 -0.5 450,793 - Atu5458 0.41 -0.3 450,803 - Atu5458 0.42 -0.5 450,851 + Atu5458 0.46 -0.4 450,955 + Atu5458 0.55 -0.6 450,963 - Atu5458 0.55 +2.3 451,006 + Atu5458 0.59 +0.2 451,021 - Atu5458 0.60 -0.3 451,021 - Atu5458 0.60 +0.9 451,041 + Atu5458 0.62 +1.2 451,188 + Atu5458 0.75 +0.3 451,194 - Atu5458 0.75 +0.1 451,255 - Atu5458 0.80 +0.3 451,285 - Atu5458 0.83 +2.3 451,329 - Atu5458 0.87 -0.1 451,410 - -1.6 451,444 - -0.9 451,444 - +0.1 451,452 + +0.1 451,531 + -1.1 451,587 + +0.3 451,595 - +1.0 451,637 - Atu5459 0.10 -0.8 451,798 + Atu5459 0.23 +0.7 451,823 + Atu5459 0.25 +1.3 451,826 - Atu5459 0.25 -0.3 451,831 - Atu5459 0.25 +0.4 451,905 + Atu5459 0.31 -1.1 451,906 + Atu5459 0.31 +0.2 451,907 + Atu5459 0.31 +0.6 451,913 - Atu5459 0.32 -0.3 451,951 + Atu5459 0.35 -0.5 452,022 + Atu5459 0.40 -0.2 452,070 + Atu5459 0.44 -0.9 452,070 + Atu5459 0.44 -1.1 452,089 + Atu5459 0.46 -0.0 452,159 - Atu5459 0.51 -0.1 452,200 + Atu5459 0.54 +0.4 452,208 - Atu5459 0.55 +1.1 452,217 + Atu5459 0.56 -0.9 452,259 - Atu5459 0.59 +2.6 452,318 + Atu5459 0.64 -0.2 452,326 - Atu5459 0.64 +1.3 452,327 - Atu5459 0.64 +0.2 452,367 + Atu5459 0.68 -1.1 452,593 - Atu5459 0.85 -1.1 452,744 - -0.3 452,751 + -0.0 452,772 + +0.2 452,788 + -0.3 452,807 + +0.2 452,824 + -3.2 452,830 - +0.7 452,935 + -1.1 452,935 + -0.9 452,943 - -2.9 452,966 - Atu5460 0.11 +0.2 452,966 - Atu5460 0.11 -0.0 452,966 - Atu5460 0.11 +0.5 453,031 - Atu5460 0.18 +3.0 453,071 + Atu5460 0.23 -3.1 453,073 + Atu5460 0.23 -0.6 453,082 - Atu5460 0.24 -0.3 453,118 + Atu5460 0.28 -0.5 453,124 + Atu5460 0.28 -0.2 453,124 + Atu5460 0.28 -1.6 453,134 + Atu5460 0.29 +0.7 453,134 + Atu5460 0.29 +4.4 453,163 + Atu5460 0.32 +0.2 453,232 - Atu5460 0.40 -0.9 453,239 + Atu5460 0.41 -0.5 453,239 + Atu5460 0.41 -0.3 453,239 + Atu5460 0.41 +1.1 453,239 + Atu5460 0.41 -1.6 453,239 + Atu5460 0.41 +0.9 453,239 + Atu5460 0.41 +0.2 453,239 + Atu5460 0.41 -1.9 453,239 + Atu5460 0.41 -0.6 453,239 + Atu5460 0.41 -0.0 453,239 + Atu5460 0.41 +0.8 453,239 + Atu5460 0.41 -0.4 453,239 + Atu5460 0.41 -2.6 453,239 + Atu5460 0.41 -0.9 453,239 + Atu5460 0.41 -0.9 453,242 - Atu5460 0.41 -2.0 453,247 - Atu5460 0.42 -0.7 453,247 - Atu5460 0.42 +0.9 453,247 - Atu5460 0.42 -0.3 453,247 - Atu5460 0.42 -0.5 453,247 - Atu5460 0.42 -1.6 453,247 - Atu5460 0.42 -0.4 453,247 - Atu5460 0.42 -0.3 453,247 - Atu5460 0.42 +2.2 453,247 - Atu5460 0.42 +3.2 453,247 - Atu5460 0.42 -0.4 453,247 - Atu5460 0.42 +0.0 453,269 + Atu5460 0.44 -0.0 453,354 - Atu5460 0.53 -0.8 453,412 - Atu5460 0.59 -0.7 453,463 - Atu5460 0.65 +0.7 453,510 - Atu5460 0.70 +0.2 453,564 - Atu5460 0.76 -0.4 453,578 + Atu5460 0.77 -0.9 453,578 + Atu5460 0.77 +0.5 453,586 - Atu5460 0.78 -0.6 453,642 + Atu5460 0.84 -0.6 453,672 - Atu5460 0.87 -1.0 453,717 + +0.4 453,726 + -2.1
Or see this region's nucleotide sequence