Strain Fitness in Escherichia coli BW25113 around b3730

Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglmS and glmU are separated by 161 nucleotidesglmU and atpC are separated by 352 nucleotidesatpC and atpD are separated by 20 nucleotides b3729: glmS - D-fructose-6-phosphate amidotransferase (NCBI), at 3,909,862 to 3,911,691 glmS b3730: glmU - bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (NCBI), at 3,911,853 to 3,913,223 glmU b3731: atpC - F0F1 ATP synthase subunit epsilon (NCBI), at 3,913,576 to 3,913,995 atpC b3732: atpD - F0F1 ATP synthase subunit beta (NCBI), at 3,914,016 to 3,915,398 atpD Position (kb) 3911 3912 3913 3914Strain fitness (log2 ratio) -2 -1 0 1 2at 3911.718 kb on + strandat 3911.759 kb on + strandat 3911.768 kb on - strandat 3911.804 kb on + strandat 3911.813 kb on - strandat 3911.813 kb on - strandat 3913.221 kb on - strandat 3913.251 kb on + strandat 3913.251 kb on + strandat 3913.500 kb on - strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.502 kb on + strandat 3913.507 kb on + strandat 3913.510 kb on - strandat 3913.510 kb on - strandat 3913.510 kb on - strandat 3913.512 kb on + strandat 3913.581 kb on - strandat 3913.993 kb on - strandat 3913.993 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM
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3,911,718 + +0.7
3,911,759 + +0.9
3,911,768 - -0.3
3,911,804 + -0.1
3,911,813 - +0.5
3,911,813 - +0.5
3,913,221 - +0.1
3,913,251 + -1.3
3,913,251 + +0.1
3,913,500 - -0.5
3,913,502 + -0.2
3,913,502 + -0.8
3,913,502 + -0.5
3,913,502 + -0.7
3,913,502 + -0.3
3,913,502 + +0.5
3,913,502 + +0.5
3,913,507 + -2.0
3,913,510 - +0.9
3,913,510 - +0.4
3,913,510 - -1.8
3,913,512 + +0.6
3,913,581 - +2.5
3,913,993 - -0.3
3,913,993 - +0.6

Or see this region's nucleotide sequence