Strain Fitness in Escherichia coli BW25113 around b2343

Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcY and b2343 are separated by 180 nucleotidesb2343 and fadL are separated by 371 nucleotides b2342: yfcY - acetyl-CoA acetyltransferase (NCBI), at 2,457,181 to 2,458,491 yfcY b2343: b2343 - orf, hypothetical protein (VIMSS), at 2,458,672 to 2,458,956 b2343 b2344: fadL - long-chain fatty acid outer membrane transporter (RefSeq), at 2,459,328 to 2,460,668 fadL Position (kb) 2458 2459Strain fitness (log2 ratio) -1 0 1at 2457.876 kb on + strand, within yfcYat 2458.221 kb on - strand, within yfcYat 2458.233 kb on - strand, within yfcYat 2458.233 kb on - strand, within yfcYat 2458.296 kb on + strand, within yfcYat 2458.452 kb on + strandat 2458.470 kb on - strandat 2458.496 kb on - strandat 2458.654 kb on + strandat 2458.859 kb on - strand, within b2343at 2458.859 kb on - strand, within b2343at 2459.436 kb on + strandat 2459.503 kb on - strand, within fadLat 2459.653 kb on + strand, within fadLat 2459.729 kb on - strand, within fadLat 2459.751 kb on + strand, within fadLat 2459.855 kb on - strand, within fadLat 2459.935 kb on + strand, within fadLat 2459.935 kb on + strand, within fadL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM
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2,457,876 + yfcY b2342 0.53 -0.2
2,458,221 - yfcY b2342 0.79 +0.4
2,458,233 - yfcY b2342 0.80 -0.1
2,458,233 - yfcY b2342 0.80 -0.3
2,458,296 + yfcY b2342 0.85 -0.6
2,458,452 + +1.0
2,458,470 - -0.1
2,458,496 - +0.1
2,458,654 + +0.6
2,458,859 - b2343 b2343 0.66 -0.2
2,458,859 - b2343 b2343 0.66 +1.0
2,459,436 + -0.4
2,459,503 - fadL b2344 0.13 -0.4
2,459,653 + fadL b2344 0.24 -0.9
2,459,729 - fadL b2344 0.30 -0.2
2,459,751 + fadL b2344 0.32 -0.6
2,459,855 - fadL b2344 0.39 -0.6
2,459,935 + fadL b2344 0.45 +0.2
2,459,935 + fadL b2344 0.45 -1.2

Or see this region's nucleotide sequence