Strain Fitness in Escherichia coli BW25113 around b2039

Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfbX and rfbC overlap by 4 nucleotidesrfbC and rfbA overlap by 1 nucleotidesrfbA and rfbD are separated by 57 nucleotidesrfbD and rfbB overlap by 1 nucleotides b2037: rfbX - predicted polisoprenol-linked O-antigen transporter (NCBI), at 2,106,361 to 2,107,608 rfbX b2038: rfbC - dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI), at 2,107,605 to 2,108,162 rfbC b2039: rfbA - glucose-1-phosphate thymidylyltransferase (NCBI), at 2,108,162 to 2,109,043 rfbA b2040: rfbD - dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI), at 2,109,101 to 2,110,000 rfbD b2041: rfbB - dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI), at 2,110,000 to 2,111,085 rfbB Position (kb) 2108 2109 2110Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2107.198 kb on - strand, within rfbXat 2107.211 kb on - strand, within rfbXat 2107.222 kb on - strand, within rfbXat 2107.249 kb on - strand, within rfbXat 2107.257 kb on - strand, within rfbXat 2107.314 kb on - strand, within rfbXat 2107.314 kb on - strand, within rfbXat 2107.319 kb on - strand, within rfbXat 2107.400 kb on - strand, within rfbXat 2107.455 kb on - strand, within rfbXat 2107.458 kb on - strand, within rfbXat 2107.460 kb on - strand, within rfbXat 2107.505 kb on - strandat 2107.505 kb on - strandat 2107.519 kb on - strandat 2107.530 kb on - strandat 2107.530 kb on - strandat 2107.574 kb on - strandat 2107.574 kb on - strandat 2107.574 kb on - strandat 2107.575 kb on - strandat 2107.575 kb on - strandat 2107.577 kb on - strandat 2107.624 kb on - strandat 2107.632 kb on - strandat 2107.632 kb on - strandat 2107.633 kb on - strandat 2107.634 kb on - strandat 2107.649 kb on - strandat 2107.649 kb on - strandat 2107.692 kb on - strand, within rfbCat 2107.692 kb on - strand, within rfbCat 2107.717 kb on - strand, within rfbCat 2107.725 kb on - strand, within rfbCat 2107.801 kb on - strand, within rfbCat 2107.801 kb on - strandat 2107.804 kb on - strand, within rfbCat 2107.811 kb on - strand, within rfbCat 2107.825 kb on + strand, within rfbCat 2107.935 kb on - strand, within rfbCat 2107.938 kb on - strand, within rfbCat 2107.938 kb on - strand, within rfbCat 2107.940 kb on - strand, within rfbCat 2107.946 kb on - strand, within rfbCat 2107.946 kb on - strand, within rfbCat 2107.948 kb on - strand, within rfbCat 2107.948 kb on - strand, within rfbCat 2107.953 kb on - strand, within rfbCat 2107.977 kb on - strand, within rfbCat 2107.992 kb on - strand, within rfbCat 2108.015 kb on - strand, within rfbCat 2108.016 kb on - strand, within rfbCat 2108.024 kb on - strand, within rfbCat 2108.027 kb on - strand, within rfbCat 2108.027 kb on - strand, within rfbCat 2108.128 kb on - strandat 2108.286 kb on - strand, within rfbAat 2108.286 kb on - strand, within rfbAat 2108.294 kb on - strand, within rfbAat 2108.300 kb on - strand, within rfbAat 2108.309 kb on - strand, within rfbAat 2108.309 kb on - strand, within rfbAat 2108.317 kb on - strand, within rfbAat 2108.317 kb on - strand, within rfbAat 2108.345 kb on - strand, within rfbAat 2108.345 kb on - strand, within rfbAat 2108.349 kb on - strand, within rfbAat 2108.349 kb on - strand, within rfbAat 2108.361 kb on - strand, within rfbAat 2108.361 kb on - strand, within rfbAat 2108.364 kb on + strand, within rfbAat 2108.366 kb on - strand, within rfbAat 2108.366 kb on - strand, within rfbAat 2108.366 kb on - strand, within rfbAat 2108.383 kb on - strand, within rfbAat 2108.383 kb on - strand, within rfbAat 2108.383 kb on - strand, within rfbAat 2108.395 kb on - strand, within rfbAat 2108.395 kb on - strand, within rfbAat 2108.397 kb on - strand, within rfbAat 2108.397 kb on - strand, within rfbAat 2108.429 kb on - strand, within rfbAat 2108.459 kb on - strand, within rfbAat 2108.463 kb on - strand, within rfbAat 2108.478 kb on - strand, within rfbAat 2108.485 kb on - strand, within rfbAat 2108.494 kb on - strand, within rfbAat 2108.494 kb on - strand, within rfbAat 2108.521 kb on + strand, within rfbAat 2108.521 kb on - strand, within rfbAat 2108.529 kb on - strand, within rfbAat 2108.564 kb on - strand, within rfbAat 2108.564 kb on - strand, within rfbAat 2108.568 kb on - strand, within rfbAat 2108.604 kb on + strand, within rfbAat 2108.610 kb on - strand, within rfbAat 2108.612 kb on - strand, within rfbAat 2108.629 kb on - strand, within rfbAat 2108.641 kb on - strand, within rfbAat 2108.641 kb on - strand, within rfbAat 2108.674 kb on + strand, within rfbAat 2108.682 kb on - strand, within rfbAat 2108.682 kb on - strand, within rfbAat 2108.682 kb on - strand, within rfbAat 2108.682 kb on - strand, within rfbAat 2108.718 kb on - strand, within rfbAat 2108.728 kb on - strand, within rfbAat 2108.728 kb on - strand, within rfbAat 2108.732 kb on + strand, within rfbAat 2108.734 kb on + strand, within rfbAat 2108.771 kb on - strand, within rfbAat 2108.790 kb on - strand, within rfbAat 2108.795 kb on - strand, within rfbAat 2108.807 kb on - strand, within rfbAat 2108.812 kb on + strand, within rfbAat 2108.814 kb on - strand, within rfbAat 2108.815 kb on - strand, within rfbAat 2108.815 kb on - strand, within rfbAat 2108.828 kb on - strand, within rfbAat 2108.828 kb on - strand, within rfbAat 2108.830 kb on - strand, within rfbAat 2108.832 kb on - strand, within rfbAat 2108.834 kb on - strand, within rfbAat 2108.835 kb on - strand, within rfbAat 2108.835 kb on - strand, within rfbAat 2108.835 kb on - strand, within rfbAat 2108.835 kb on - strand, within rfbAat 2108.835 kb on - strand, within rfbAat 2108.853 kb on - strand, within rfbAat 2108.864 kb on + strand, within rfbAat 2108.864 kb on + strand, within rfbAat 2108.864 kb on + strand, within rfbAat 2108.864 kb on + strand, within rfbAat 2108.870 kb on - strand, within rfbAat 2108.870 kb on - strand, within rfbAat 2108.874 kb on + strand, within rfbAat 2108.874 kb on + strand, within rfbAat 2108.913 kb on + strand, within rfbAat 2108.913 kb on + strand, within rfbAat 2108.922 kb on - strand, within rfbAat 2108.922 kb on - strand, within rfbAat 2108.924 kb on - strand, within rfbAat 2108.924 kb on - strand, within rfbAat 2108.926 kb on + strand, within rfbAat 2108.953 kb on - strand, within rfbAat 2108.956 kb on - strandat 2108.957 kb on - strandat 2108.973 kb on - strandat 2108.977 kb on - strandat 2108.977 kb on - strandat 2108.982 kb on - strandat 2108.982 kb on - strandat 2108.996 kb on - strandat 2108.996 kb on - strandat 2108.996 kb on - strandat 2108.996 kb on - strandat 2108.998 kb on - strandat 2109.009 kb on - strandat 2109.076 kb on - strandat 2109.148 kb on - strandat 2109.148 kb on - strandat 2109.148 kb on - strandat 2109.159 kb on - strandat 2109.159 kb on - strandat 2109.166 kb on - strandat 2109.234 kb on + strand, within rfbDat 2109.236 kb on + strand, within rfbDat 2109.239 kb on - strandat 2109.239 kb on - strand, within rfbDat 2109.240 kb on - strand, within rfbDat 2109.253 kb on + strand, within rfbDat 2109.253 kb on + strand, within rfbDat 2109.254 kb on - strand, within rfbDat 2109.266 kb on - strand, within rfbDat 2109.272 kb on - strand, within rfbDat 2109.274 kb on + strand, within rfbDat 2109.299 kb on - strand, within rfbDat 2109.299 kb on - strand, within rfbDat 2109.329 kb on - strand, within rfbDat 2109.329 kb on - strand, within rfbDat 2109.329 kb on - strand, within rfbDat 2109.336 kb on + strand, within rfbDat 2109.344 kb on - strand, within rfbDat 2109.353 kb on - strand, within rfbDat 2109.360 kb on - strand, within rfbDat 2109.362 kb on + strand, within rfbDat 2109.362 kb on + strand, within rfbDat 2109.370 kb on - strand, within rfbDat 2109.370 kb on - strand, within rfbDat 2109.370 kb on - strand, within rfbDat 2109.370 kb on - strand, within rfbDat 2109.370 kb on - strand, within rfbDat 2109.375 kb on - strand, within rfbDat 2109.409 kb on + strand, within rfbDat 2109.412 kb on - strand, within rfbDat 2109.412 kb on - strand, within rfbDat 2109.412 kb on - strand, within rfbDat 2109.412 kb on - strand, within rfbDat 2109.421 kb on + strand, within rfbDat 2109.430 kb on + strand, within rfbDat 2109.438 kb on - strand, within rfbDat 2109.438 kb on - strand, within rfbDat 2109.521 kb on + strand, within rfbDat 2109.521 kb on + strand, within rfbDat 2109.653 kb on - strand, within rfbDat 2109.653 kb on - strand, within rfbDat 2109.653 kb on - strand, within rfbDat 2109.698 kb on - strand, within rfbDat 2109.726 kb on - strand, within rfbDat 2109.742 kb on + strand, within rfbDat 2109.742 kb on + strand, within rfbDat 2109.742 kb on + strand, within rfbDat 2109.767 kb on - strand, within rfbDat 2109.797 kb on + strand, within rfbDat 2109.797 kb on + strand, within rfbDat 2109.805 kb on - strand, within rfbDat 2109.805 kb on - strand, within rfbDat 2109.805 kb on - strand, within rfbDat 2109.805 kb on - strand, within rfbDat 2109.805 kb on - strand, within rfbDat 2109.815 kb on - strand, within rfbDat 2109.815 kb on - strand, within rfbDat 2109.861 kb on + strand, within rfbDat 2109.908 kb on - strand, within rfbDat 2109.936 kb on + strandat 2109.936 kb on + strandat 2109.943 kb on + strandat 2109.945 kb on + strandat 2109.945 kb on - strandat 2109.947 kb on + strandat 2109.958 kb on - strandat 2109.958 kb on - strandat 2109.958 kb on - strandat 2109.960 kb on - strandat 2109.963 kb on - strandat 2109.963 kb on - strandat 2109.963 kb on - strandat 2109.963 kb on - strandat 2109.965 kb on + strandat 2109.965 kb on - strandat 2109.995 kb on + strandat 2110.018 kb on - strandat 2110.036 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM
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2,107,198 - rfbX b2037 0.67 +2.8
2,107,211 - rfbX b2037 0.68 -0.4
2,107,222 - rfbX b2037 0.69 +1.8
2,107,249 - rfbX b2037 0.71 +0.2
2,107,257 - rfbX b2037 0.72 +1.2
2,107,314 - rfbX b2037 0.76 +0.2
2,107,314 - rfbX b2037 0.76 +0.2
2,107,319 - rfbX b2037 0.77 +1.2
2,107,400 - rfbX b2037 0.83 -1.8
2,107,455 - rfbX b2037 0.88 +1.2
2,107,458 - rfbX b2037 0.88 +1.5
2,107,460 - rfbX b2037 0.88 +2.2
2,107,505 - -1.4
2,107,505 - +0.8
2,107,519 - -0.6
2,107,530 - +1.2
2,107,530 - +1.2
2,107,574 - -0.4
2,107,574 - +2.8
2,107,574 - -0.4
2,107,575 - +1.4
2,107,575 - -0.4
2,107,577 - +0.8
2,107,624 - +0.2
2,107,632 - +0.2
2,107,632 - +0.6
2,107,633 - -0.6
2,107,634 - -2.4
2,107,649 - -0.6
2,107,649 - +1.2
2,107,692 - rfbC b2038 0.16 -0.8
2,107,692 - rfbC b2038 0.16 -0.1
2,107,717 - rfbC b2038 0.20 -1.1
2,107,725 - rfbC b2038 0.22 -0.0
2,107,801 - rfbC b2038 0.35 +0.2
2,107,801 - +1.2
2,107,804 - rfbC b2038 0.36 -1.3
2,107,811 - rfbC b2038 0.37 -1.6
2,107,825 + rfbC b2038 0.39 +1.2
2,107,935 - rfbC b2038 0.59 -0.4
2,107,938 - rfbC b2038 0.60 -2.5
2,107,938 - rfbC b2038 0.60 -0.2
2,107,940 - rfbC b2038 0.60 -1.0
2,107,946 - rfbC b2038 0.61 -1.6
2,107,946 - rfbC b2038 0.61 -0.4
2,107,948 - rfbC b2038 0.61 -0.7
2,107,948 - rfbC b2038 0.61 -0.8
2,107,953 - rfbC b2038 0.62 -1.0
2,107,977 - rfbC b2038 0.67 -0.4
2,107,992 - rfbC b2038 0.69 -1.6
2,108,015 - rfbC b2038 0.73 +1.8
2,108,016 - rfbC b2038 0.74 +0.6
2,108,024 - rfbC b2038 0.75 +0.8
2,108,027 - rfbC b2038 0.76 +0.2
2,108,027 - rfbC b2038 0.76 +0.2
2,108,128 - +0.2
2,108,286 - rfbA b2039 0.14 -2.0
2,108,286 - rfbA b2039 0.14 +0.1
2,108,294 - rfbA b2039 0.15 -0.1
2,108,300 - rfbA b2039 0.16 -0.4
2,108,309 - rfbA b2039 0.17 -0.1
2,108,309 - rfbA b2039 0.17 +0.3
2,108,317 - rfbA b2039 0.18 +1.3
2,108,317 - rfbA b2039 0.18 +0.2
2,108,345 - rfbA b2039 0.21 -0.4
2,108,345 - rfbA b2039 0.21 +0.4
2,108,349 - rfbA b2039 0.21 +0.6
2,108,349 - rfbA b2039 0.21 +0.6
2,108,361 - rfbA b2039 0.23 +3.0
2,108,361 - rfbA b2039 0.23 -0.2
2,108,364 + rfbA b2039 0.23 +1.2
2,108,366 - rfbA b2039 0.23 -0.1
2,108,366 - rfbA b2039 0.23 +1.2
2,108,366 - rfbA b2039 0.23 +0.2
2,108,383 - rfbA b2039 0.25 +0.4
2,108,383 - rfbA b2039 0.25 +0.2
2,108,383 - rfbA b2039 0.25 +1.2
2,108,395 - rfbA b2039 0.26 -0.1
2,108,395 - rfbA b2039 0.26 -0.4
2,108,397 - rfbA b2039 0.27 -1.6
2,108,397 - rfbA b2039 0.27 -0.8
2,108,429 - rfbA b2039 0.30 +0.2
2,108,459 - rfbA b2039 0.34 +0.2
2,108,463 - rfbA b2039 0.34 +0.0
2,108,478 - rfbA b2039 0.36 -0.0
2,108,485 - rfbA b2039 0.37 +0.1
2,108,494 - rfbA b2039 0.38 +0.2
2,108,494 - rfbA b2039 0.38 -0.8
2,108,521 + rfbA b2039 0.41 +1.2
2,108,521 - rfbA b2039 0.41 +1.2
2,108,529 - rfbA b2039 0.42 -1.6
2,108,564 - rfbA b2039 0.46 +0.6
2,108,564 - rfbA b2039 0.46 -0.6
2,108,568 - rfbA b2039 0.46 +1.4
2,108,604 + rfbA b2039 0.50 +1.2
2,108,610 - rfbA b2039 0.51 +0.0
2,108,612 - rfbA b2039 0.51 -0.3
2,108,629 - rfbA b2039 0.53 +1.4
2,108,641 - rfbA b2039 0.54 -0.4
2,108,641 - rfbA b2039 0.54 +0.2
2,108,674 + rfbA b2039 0.58 +1.2
2,108,682 - rfbA b2039 0.59 -0.6
2,108,682 - rfbA b2039 0.59 +0.4
2,108,682 - rfbA b2039 0.59 +1.2
2,108,682 - rfbA b2039 0.59 -1.3
2,108,718 - rfbA b2039 0.63 -1.1
2,108,728 - rfbA b2039 0.64 -0.4
2,108,728 - rfbA b2039 0.64 -0.6
2,108,732 + rfbA b2039 0.65 +1.2
2,108,734 + rfbA b2039 0.65 +1.2
2,108,771 - rfbA b2039 0.69 -0.3
2,108,790 - rfbA b2039 0.71 +1.2
2,108,795 - rfbA b2039 0.72 +0.2
2,108,807 - rfbA b2039 0.73 +0.7
2,108,812 + rfbA b2039 0.74 +1.2
2,108,814 - rfbA b2039 0.74 +0.2
2,108,815 - rfbA b2039 0.74 -0.1
2,108,815 - rfbA b2039 0.74 -2.0
2,108,828 - rfbA b2039 0.76 +0.2
2,108,828 - rfbA b2039 0.76 +0.8
2,108,830 - rfbA b2039 0.76 +0.4
2,108,832 - rfbA b2039 0.76 -2.0
2,108,834 - rfbA b2039 0.76 -1.0
2,108,835 - rfbA b2039 0.76 -0.5
2,108,835 - rfbA b2039 0.76 +0.3
2,108,835 - rfbA b2039 0.76 +0.0
2,108,835 - rfbA b2039 0.76 +0.8
2,108,835 - rfbA b2039 0.76 -1.1
2,108,853 - rfbA b2039 0.78 +0.2
2,108,864 + rfbA b2039 0.80 -0.8
2,108,864 + rfbA b2039 0.80 -1.6
2,108,864 + rfbA b2039 0.80 +1.2
2,108,864 + rfbA b2039 0.80 +0.1
2,108,870 - rfbA b2039 0.80 +0.1
2,108,870 - rfbA b2039 0.80 +1.0
2,108,874 + rfbA b2039 0.81 -0.8
2,108,874 + rfbA b2039 0.81 -0.6
2,108,913 + rfbA b2039 0.85 +1.2
2,108,913 + rfbA b2039 0.85 +1.2
2,108,922 - rfbA b2039 0.86 -0.1
2,108,922 - rfbA b2039 0.86 -0.3
2,108,924 - rfbA b2039 0.86 -1.4
2,108,924 - rfbA b2039 0.86 +1.2
2,108,926 + rfbA b2039 0.87 +1.2
2,108,953 - rfbA b2039 0.90 -0.2
2,108,956 - -0.6
2,108,957 - -0.4
2,108,973 - -0.7
2,108,977 - +3.2
2,108,977 - -2.4
2,108,982 - -0.2
2,108,982 - +0.4
2,108,996 - +0.2
2,108,996 - +1.2
2,108,996 - +0.0
2,108,996 - -0.6
2,108,998 - -0.3
2,109,009 - -0.6
2,109,076 - -0.9
2,109,148 - -0.4
2,109,148 - +0.8
2,109,148 - -2.2
2,109,159 - -0.1
2,109,159 - +0.3
2,109,166 - -0.8
2,109,234 + rfbD b2040 0.15 +1.2
2,109,236 + rfbD b2040 0.15 +1.2
2,109,239 - -0.4
2,109,239 - rfbD b2040 0.15 -1.1
2,109,240 - rfbD b2040 0.15 -1.4
2,109,253 + rfbD b2040 0.17 +1.2
2,109,253 + rfbD b2040 0.17 +1.2
2,109,254 - rfbD b2040 0.17 -0.4
2,109,266 - rfbD b2040 0.18 -0.8
2,109,272 - rfbD b2040 0.19 -1.8
2,109,274 + rfbD b2040 0.19 +1.2
2,109,299 - rfbD b2040 0.22 -1.6
2,109,299 - rfbD b2040 0.22 -2.7
2,109,329 - rfbD b2040 0.25 +0.2
2,109,329 - rfbD b2040 0.25 +1.2
2,109,329 - rfbD b2040 0.25 -1.4
2,109,336 + rfbD b2040 0.26 +0.2
2,109,344 - rfbD b2040 0.27 -1.3
2,109,353 - rfbD b2040 0.28 -1.1
2,109,360 - rfbD b2040 0.29 -1.7
2,109,362 + rfbD b2040 0.29 +0.2
2,109,362 + rfbD b2040 0.29 +1.2
2,109,370 - rfbD b2040 0.30 -1.4
2,109,370 - rfbD b2040 0.30 -1.8
2,109,370 - rfbD b2040 0.30 -1.3
2,109,370 - rfbD b2040 0.30 -1.3
2,109,370 - rfbD b2040 0.30 -0.6
2,109,375 - rfbD b2040 0.30 -0.3
2,109,409 + rfbD b2040 0.34 -1.1
2,109,412 - rfbD b2040 0.35 -0.6
2,109,412 - rfbD b2040 0.35 -0.1
2,109,412 - rfbD b2040 0.35 -0.4
2,109,412 - rfbD b2040 0.35 -1.3
2,109,421 + rfbD b2040 0.36 -1.1
2,109,430 + rfbD b2040 0.37 +1.2
2,109,438 - rfbD b2040 0.37 -1.1
2,109,438 - rfbD b2040 0.37 -1.7
2,109,521 + rfbD b2040 0.47 -1.4
2,109,521 + rfbD b2040 0.47 +0.2
2,109,653 - rfbD b2040 0.61 -1.8
2,109,653 - rfbD b2040 0.61 -1.1
2,109,653 - rfbD b2040 0.61 +1.2
2,109,698 - rfbD b2040 0.66 -2.0
2,109,726 - rfbD b2040 0.69 -1.5
2,109,742 + rfbD b2040 0.71 -0.8
2,109,742 + rfbD b2040 0.71 -0.4
2,109,742 + rfbD b2040 0.71 -0.4
2,109,767 - rfbD b2040 0.74 -1.9
2,109,797 + rfbD b2040 0.77 +1.2
2,109,797 + rfbD b2040 0.77 +0.2
2,109,805 - rfbD b2040 0.78 -0.4
2,109,805 - rfbD b2040 0.78 -2.4
2,109,805 - rfbD b2040 0.78 -0.8
2,109,805 - rfbD b2040 0.78 +0.6
2,109,805 - rfbD b2040 0.78 -1.4
2,109,815 - rfbD b2040 0.79 -1.4
2,109,815 - rfbD b2040 0.79 +0.2
2,109,861 + rfbD b2040 0.84 +1.2
2,109,908 - rfbD b2040 0.90 +0.2
2,109,936 + +0.2
2,109,936 + +0.2
2,109,943 + +1.2
2,109,945 + +0.2
2,109,945 - +0.6
2,109,947 + +1.2
2,109,958 - -2.8
2,109,958 - -0.7
2,109,958 - -1.3
2,109,960 - -0.4
2,109,963 - -2.4
2,109,963 - -2.1
2,109,963 - +1.2
2,109,963 - -0.2
2,109,965 + +1.2
2,109,965 - -1.3
2,109,995 + -0.4
2,110,018 - -1.2
2,110,036 - -0.8

Or see this region's nucleotide sequence