Experiment: Adaptation in M9 media timecourse 3; day 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3415 and PP_3416 are separated by 206 nucleotides PP_3416 and PP_3417 are separated by 111 nucleotides PP_3417 and PP_3418 are separated by 86 nucleotides PP_3418 and PP_3419 are separated by 14 nucleotides
PP_3415: PP_3415 - Transcriptional regulator, LacI family, at 3,865,858 to 3,866,877
_3415
PP_3416: PP_3416 - D-gluconate kinase, at 3,867,084 to 3,867,623
_3416
PP_3417: PP_3417 - D-gluconate transporter, at 3,867,735 to 3,869,087
_3417
PP_3418: PP_3418 - conserved protein of unknown function, at 3,869,174 to 3,869,476
_3418
PP_3419: PP_3419 - Sigma-54 dependent transcriptional regulator/response regulator, at 3,869,491 to 3,870,918
_3419
Position (kb)
3867
3868
3869
3870 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3866.853 kb on + strand at 3866.854 kb on - strand at 3866.854 kb on - strand at 3866.854 kb on - strand at 3866.892 kb on + strand at 3866.921 kb on + strand at 3866.922 kb on - strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.953 kb on + strand at 3866.954 kb on - strand at 3866.997 kb on + strand at 3866.998 kb on - strand at 3867.020 kb on - strand at 3867.020 kb on - strand at 3867.079 kb on + strand at 3867.079 kb on + strand at 3867.080 kb on - strand at 3867.584 kb on - strand at 3867.714 kb on + strand at 3867.715 kb on - strand at 3867.715 kb on - strand at 3867.750 kb on - strand at 3867.750 kb on - strand at 3867.783 kb on - strand at 3867.783 kb on - strand at 3867.928 kb on + strand, within PP_3417 at 3867.928 kb on + strand, within PP_3417 at 3868.287 kb on + strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.440 kb on - strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.453 kb on + strand, within PP_3417 at 3868.454 kb on - strand, within PP_3417 at 3868.856 kb on + strand, within PP_3417 at 3868.909 kb on + strand, within PP_3417 at 3868.981 kb on - strand at 3868.981 kb on - strand at 3868.981 kb on - strand at 3869.101 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.156 kb on - strand at 3869.156 kb on - strand at 3869.347 kb on - strand, within PP_3418 at 3869.467 kb on + strand at 3869.490 kb on - strand at 3869.490 kb on - strand at 3869.492 kb on + strand at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419
Per-strain Table
Position Strand Gene LocusTag Fraction Adaptation in M9 media timecourse 3; day 2 remove 3,866,853 + -0.7 3,866,854 - -0.2 3,866,854 - +0.3 3,866,854 - -0.3 3,866,892 + -0.4 3,866,921 + +0.2 3,866,922 - +0.4 3,866,953 + +0.1 3,866,953 + -1.6 3,866,953 + -0.1 3,866,953 + -0.5 3,866,954 - +0.0 3,866,997 + -0.4 3,866,998 - -1.4 3,867,020 - -0.0 3,867,020 - -0.4 3,867,079 + +0.3 3,867,079 + -0.3 3,867,080 - -0.4 3,867,584 - -0.8 3,867,714 + +0.4 3,867,715 - -1.4 3,867,715 - +1.0 3,867,750 - +3.8 3,867,750 - +1.4 3,867,783 - -0.1 3,867,783 - -0.4 3,867,928 + PP_3417 0.14 -1.1 3,867,928 + PP_3417 0.14 -0.6 3,868,287 + PP_3417 0.41 -0.3 3,868,440 - PP_3417 0.52 +0.2 3,868,440 - PP_3417 0.52 +0.9 3,868,440 - PP_3417 0.52 -1.3 3,868,453 + PP_3417 0.53 -0.5 3,868,453 + PP_3417 0.53 -0.6 3,868,453 + PP_3417 0.53 -0.7 3,868,454 - PP_3417 0.53 +0.5 3,868,856 + PP_3417 0.83 -0.7 3,868,909 + PP_3417 0.87 +0.1 3,868,981 - -1.0 3,868,981 - -0.3 3,868,981 - -1.1 3,869,101 + +0.7 3,869,140 + -0.6 3,869,140 + -0.2 3,869,140 + -0.2 3,869,141 - -1.4 3,869,141 - -1.4 3,869,141 - -0.7 3,869,141 - -0.6 3,869,156 - +0.1 3,869,156 - -0.4 3,869,347 - PP_3418 0.57 +0.3 3,869,467 + -0.3 3,869,490 - -0.4 3,869,490 - +0.7 3,869,492 + -0.2 3,869,832 + PP_3419 0.24 +0.0 3,869,832 + PP_3419 0.24 +0.3 3,869,832 + PP_3419 0.24 +0.0 3,869,832 + PP_3419 0.24 -0.3 3,869,833 - PP_3419 0.24 +0.1 3,869,833 - PP_3419 0.24 +0.4
Or see this region's nucleotide sequence