Strain Fitness in Pseudomonas putida KT2440 around PP_0822

Experiment: Adaptation in M9 media timecourse 3; day 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5460 and PP_0821 overlap by 4 nucleotidesPP_0821 and PP_0822 are separated by 125 nucleotidesPP_0822 and PP_0823 overlap by 1 nucleotides PP_5460: PP_5460 - conserved membrane protein of unknown function, at 959,196 to 959,735 _5460 PP_0821: PP_0821 - conserved exported protein of unknown function, at 959,732 to 960,142 _0821 PP_0822: PP_0822 - tRNA 2-selenouridine synthase, at 960,268 to 961,380 _0822 PP_0823: PP_0823 - Selenide, water dikinase, at 961,380 to 962,414 _0823 Position (kb) 960 961 962Strain fitness (log2 ratio) -2 -1 0 1 2 3at 959.282 kb on + strand, within PP_5460at 959.283 kb on - strand, within PP_5460at 959.338 kb on - strand, within PP_5460at 959.430 kb on + strand, within PP_5460at 959.431 kb on - strand, within PP_5460at 959.481 kb on + strand, within PP_5460at 959.482 kb on - strand, within PP_5460at 959.482 kb on - strand, within PP_5460at 959.520 kb on + strand, within PP_5460at 959.521 kb on - strandat 959.655 kb on + strand, within PP_5460at 959.655 kb on + strand, within PP_5460at 959.655 kb on + strand, within PP_5460at 959.656 kb on - strand, within PP_5460at 959.752 kb on + strandat 959.769 kb on - strandat 959.769 kb on - strandat 959.769 kb on - strandat 959.822 kb on + strand, within PP_0821at 960.006 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.127 kb on + strandat 960.261 kb on - strandat 960.269 kb on + strandat 960.270 kb on - strandat 960.270 kb on - strandat 960.305 kb on + strandat 960.306 kb on - strandat 960.306 kb on - strandat 960.314 kb on - strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.374 kb on + strandat 960.375 kb on - strandat 960.375 kb on - strandat 960.375 kb on - strandat 960.375 kb on - strandat 960.386 kb on + strand, within PP_0822at 960.387 kb on - strand, within PP_0822at 960.389 kb on + strand, within PP_0822at 960.390 kb on - strand, within PP_0822at 960.430 kb on + strand, within PP_0822at 960.430 kb on + strand, within PP_0822at 960.515 kb on + strand, within PP_0822at 960.516 kb on - strand, within PP_0822at 960.519 kb on + strand, within PP_0822at 960.519 kb on + strand, within PP_0822at 960.519 kb on + strand, within PP_0822at 960.519 kb on + strand, within PP_0822at 960.519 kb on + strand, within PP_0822at 960.520 kb on - strand, within PP_0822at 960.614 kb on + strand, within PP_0822at 960.692 kb on + strand, within PP_0822at 960.692 kb on + strand, within PP_0822at 960.693 kb on - strand, within PP_0822at 960.978 kb on + strand, within PP_0822at 960.996 kb on + strand, within PP_0822at 960.996 kb on + strand, within PP_0822at 960.996 kb on + strand, within PP_0822at 960.996 kb on + strand, within PP_0822at 960.997 kb on - strand, within PP_0822at 961.010 kb on + strand, within PP_0822at 961.039 kb on - strandat 961.039 kb on - strand, within PP_0822at 961.224 kb on - strand, within PP_0822at 961.273 kb on + strandat 961.497 kb on + strand, within PP_0823at 961.497 kb on + strand, within PP_0823at 961.498 kb on - strand, within PP_0823at 961.576 kb on + strand, within PP_0823at 961.577 kb on - strand, within PP_0823at 961.585 kb on + strand, within PP_0823at 961.640 kb on + strand, within PP_0823at 961.641 kb on - strand, within PP_0823at 961.664 kb on - strand, within PP_0823at 961.664 kb on - strand, within PP_0823at 961.664 kb on - strand, within PP_0823at 961.895 kb on - strand, within PP_0823at 961.895 kb on - strand, within PP_0823at 961.943 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.140 kb on + strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.141 kb on - strand, within PP_0823at 962.185 kb on + strand, within PP_0823

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Per-strain Table

Position Strand Gene LocusTag Fraction Adaptation in M9 media timecourse 3; day 2
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959,282 + PP_5460 0.16 -0.3
959,283 - PP_5460 0.16 +1.0
959,338 - PP_5460 0.26 +0.4
959,430 + PP_5460 0.43 +0.1
959,431 - PP_5460 0.44 +0.0
959,481 + PP_5460 0.53 +0.5
959,482 - PP_5460 0.53 -0.6
959,482 - PP_5460 0.53 +0.1
959,520 + PP_5460 0.60 -0.0
959,521 - -0.2
959,655 + PP_5460 0.85 -0.1
959,655 + PP_5460 0.85 -0.1
959,655 + PP_5460 0.85 -0.1
959,656 - PP_5460 0.85 +0.6
959,752 + +0.2
959,769 - -0.3
959,769 - -0.0
959,769 - +1.9
959,822 + PP_0821 0.22 +0.4
960,006 - PP_0821 0.67 -0.2
960,034 - PP_0821 0.73 -0.2
960,034 - PP_0821 0.73 +0.3
960,034 - PP_0821 0.73 +0.3
960,034 - PP_0821 0.73 -0.2
960,127 + -0.4
960,261 - +0.0
960,269 + +0.4
960,270 - +0.3
960,270 - -0.2
960,305 + -1.3
960,306 - +0.1
960,306 - -1.0
960,314 - +0.0
960,374 + +0.4
960,374 + +0.6
960,374 + +0.4
960,374 + -0.7
960,374 + -0.7
960,374 + -1.2
960,374 + +1.4
960,374 + -0.2
960,374 + -0.5
960,374 + +0.1
960,374 + -1.2
960,375 - -0.3
960,375 - -0.4
960,375 - +0.8
960,375 - -0.1
960,386 + PP_0822 0.11 +0.1
960,387 - PP_0822 0.11 -0.7
960,389 + PP_0822 0.11 +0.5
960,390 - PP_0822 0.11 +1.6
960,430 + PP_0822 0.15 +0.2
960,430 + PP_0822 0.15 -0.1
960,515 + PP_0822 0.22 -0.5
960,516 - PP_0822 0.22 +0.7
960,519 + PP_0822 0.23 -0.4
960,519 + PP_0822 0.23 +0.8
960,519 + PP_0822 0.23 +0.6
960,519 + PP_0822 0.23 -0.1
960,519 + PP_0822 0.23 +0.6
960,520 - PP_0822 0.23 -0.6
960,614 + PP_0822 0.31 -0.1
960,692 + PP_0822 0.38 +0.4
960,692 + PP_0822 0.38 +3.0
960,693 - PP_0822 0.38 -0.3
960,978 + PP_0822 0.64 -0.0
960,996 + PP_0822 0.65 +0.7
960,996 + PP_0822 0.65 +0.6
960,996 + PP_0822 0.65 -0.2
960,996 + PP_0822 0.65 -0.3
960,997 - PP_0822 0.65 +0.6
961,010 + PP_0822 0.67 +0.7
961,039 - -0.2
961,039 - PP_0822 0.69 +0.3
961,224 - PP_0822 0.86 -0.4
961,273 + +0.1
961,497 + PP_0823 0.11 -1.4
961,497 + PP_0823 0.11 +0.4
961,498 - PP_0823 0.11 -0.4
961,576 + PP_0823 0.19 -0.4
961,577 - PP_0823 0.19 +0.2
961,585 + PP_0823 0.20 +1.4
961,640 + PP_0823 0.25 -0.6
961,641 - PP_0823 0.25 -0.2
961,664 - PP_0823 0.27 +0.0
961,664 - PP_0823 0.27 +0.2
961,664 - PP_0823 0.27 +0.7
961,895 - PP_0823 0.50 +0.2
961,895 - PP_0823 0.50 +0.4
961,943 + PP_0823 0.54 +0.7
962,140 + PP_0823 0.73 -0.3
962,140 + PP_0823 0.73 -0.3
962,140 + PP_0823 0.73 -0.7
962,140 + PP_0823 0.73 -0.1
962,140 + PP_0823 0.73 +0.5
962,140 + PP_0823 0.73 +0.2
962,140 + PP_0823 0.73 +0.3
962,140 + PP_0823 0.73 +0.2
962,140 + PP_0823 0.73 -1.8
962,141 - PP_0823 0.74 +0.4
962,141 - PP_0823 0.74 -0.4
962,141 - PP_0823 0.74 -1.7
962,141 - PP_0823 0.74 +0.2
962,141 - PP_0823 0.74 +0.6
962,141 - PP_0823 0.74 -0.3
962,141 - PP_0823 0.74 -1.2
962,141 - PP_0823 0.74 +1.4
962,141 - PP_0823 0.74 +0.2
962,141 - PP_0823 0.74 +0.1
962,185 + PP_0823 0.78 -0.2

Or see this region's nucleotide sequence