Experiment: Adaptation in M9 media timecourse 3; day 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0229 and PP_0230 are separated by 62 nucleotides PP_0230 and PP_0231 are separated by 48 nucleotides PP_0231 and PP_0232 overlap by 4 nucleotides PP_0232 and PP_0233 are separated by 13 nucleotides
PP_0229: PP_0229 - choline/carnitine/betaine transporter, at 282,550 to 284,553
_0229
PP_0230: PP_0230 - alpha-ketoglutarate-dependent sulfonate dioxygenase, at 284,616 to 285,449
_0230
PP_0231: PP_0231 - taurine ABC transporter permease subunit, at 285,498 to 286,337
_0231
PP_0232: PP_0232 - ATP-binding taurine transporter subunit, at 286,334 to 287,122
_0232
PP_0233: PP_0233 - taurine ABC transporter periplasmic binding subunit, at 287,136 to 288,107
_0233
Position (kb) 285
286
287 Strain fitness (log2 ratio) -1
0
1
2 at 284.533 kb on + strand at 284.533 kb on + strand at 284.533 kb on + strand at 285.092 kb on - strand, within PP_0230 at 285.377 kb on + strand at 285.823 kb on - strand, within PP_0231 at 285.877 kb on + strand, within PP_0231 at 285.878 kb on - strand, within PP_0231 at 285.878 kb on - strand, within PP_0231 at 286.007 kb on - strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.114 kb on + strand, within PP_0231 at 286.115 kb on - strand, within PP_0231 at 286.218 kb on + strand, within PP_0231 at 286.512 kb on - strand, within PP_0232 at 287.085 kb on + strand at 287.164 kb on + strand at 287.164 kb on + strand at 287.164 kb on + strand at 287.165 kb on - strand at 287.165 kb on - strand at 287.288 kb on - strand, within PP_0233
Per-strain Table
Position Strand Gene LocusTag Fraction Adaptation in M9 media timecourse 3; day 2 remove 284,533 + -1.1 284,533 + +0.6 284,533 + +2.4 285,092 - PP_0230 0.57 -0.8 285,377 + +0.5 285,823 - PP_0231 0.39 -0.2 285,877 + PP_0231 0.45 +0.1 285,878 - PP_0231 0.45 -0.8 285,878 - PP_0231 0.45 -0.8 286,007 - PP_0231 0.61 -0.9 286,114 + PP_0231 0.73 +1.4 286,114 + PP_0231 0.73 -0.7 286,114 + PP_0231 0.73 -0.6 286,114 + PP_0231 0.73 -0.2 286,114 + PP_0231 0.73 -0.6 286,114 + PP_0231 0.73 -0.1 286,115 - PP_0231 0.73 -1.3 286,218 + PP_0231 0.86 -0.2 286,512 - PP_0232 0.23 -0.2 287,085 + -0.3 287,164 + -0.5 287,164 + -0.1 287,164 + +0.5 287,165 - -1.1 287,165 - -0.2 287,288 - PP_0233 0.16 +0.2
Or see this region's nucleotide sequence