Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4309 and Atu4310 are separated by 99 nucleotides Atu4310 and Atu4311 are separated by 66 nucleotides Atu4311 and Atu4312 overlap by 4 nucleotides
Atu4309: Atu4309 - AraC family transcriptional regulator, at 1,434,893 to 1,435,924
Atu4309
Atu4310: Atu4310 - sarcosine oxidase beta subunit, at 1,436,024 to 1,437,277
Atu4310
Atu4311: Atu4311 - sarcosine oxidase delta subunit, at 1,437,344 to 1,437,664
Atu4311
Atu4312: Atu4312 - sarcosine oxidase alpha subunit, at 1,437,661 to 1,440,651
Atu4312
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1435.060 kb on + strand, within Atu4309 at 1435.068 kb on - strand, within Atu4309 at 1435.081 kb on - strand, within Atu4309 at 1435.110 kb on + strand, within Atu4309 at 1435.169 kb on - strand, within Atu4309 at 1435.171 kb on + strand, within Atu4309 at 1435.185 kb on - strand, within Atu4309 at 1435.264 kb on + strand, within Atu4309 at 1435.283 kb on + strand, within Atu4309 at 1435.432 kb on - strand, within Atu4309 at 1435.474 kb on - strand, within Atu4309 at 1435.474 kb on - strand, within Atu4309 at 1435.543 kb on + strand, within Atu4309 at 1435.649 kb on + strand, within Atu4309 at 1435.693 kb on + strand, within Atu4309 at 1435.772 kb on - strand, within Atu4309 at 1435.814 kb on + strand, within Atu4309 at 1435.826 kb on + strand at 1435.826 kb on + strand at 1436.009 kb on + strand at 1436.334 kb on + strand, within Atu4310 at 1436.437 kb on + strand, within Atu4310 at 1436.448 kb on + strand, within Atu4310 at 1436.498 kb on + strand, within Atu4310 at 1436.498 kb on + strand, within Atu4310 at 1436.498 kb on + strand, within Atu4310 at 1436.506 kb on - strand, within Atu4310 at 1436.506 kb on - strand, within Atu4310 at 1436.506 kb on - strand, within Atu4310 at 1436.529 kb on - strand, within Atu4310 at 1436.531 kb on + strand, within Atu4310 at 1436.539 kb on - strand, within Atu4310 at 1436.539 kb on - strand, within Atu4310 at 1436.584 kb on + strand, within Atu4310 at 1436.593 kb on - strand, within Atu4310 at 1436.630 kb on + strand, within Atu4310 at 1436.638 kb on - strand, within Atu4310 at 1436.667 kb on - strand, within Atu4310 at 1436.814 kb on + strand, within Atu4310 at 1436.864 kb on + strand, within Atu4310 at 1436.921 kb on + strand, within Atu4310 at 1436.921 kb on + strand, within Atu4310 at 1436.921 kb on + strand, within Atu4310 at 1436.921 kb on + strand, within Atu4310 at 1436.921 kb on + strand, within Atu4310 at 1436.929 kb on - strand, within Atu4310 at 1436.931 kb on - strand, within Atu4310 at 1436.939 kb on - strand, within Atu4310 at 1437.033 kb on - strand, within Atu4310 at 1437.049 kb on + strand, within Atu4310 at 1437.070 kb on - strand, within Atu4310 at 1437.082 kb on + strand, within Atu4310 at 1437.152 kb on + strand at 1437.152 kb on + strand at 1437.152 kb on + strand at 1437.160 kb on - strand at 1437.160 kb on - strand at 1437.160 kb on - strand at 1437.160 kb on - strand at 1437.160 kb on - strand at 1437.166 kb on - strand at 1437.213 kb on + strand at 1437.412 kb on + strand, within Atu4311 at 1437.429 kb on + strand, within Atu4311 at 1437.434 kb on + strand, within Atu4311 at 1437.458 kb on - strand, within Atu4311 at 1437.475 kb on - strand, within Atu4311 at 1437.505 kb on - strand, within Atu4311 at 1437.529 kb on - strand, within Atu4311 at 1437.552 kb on - strand, within Atu4311 at 1437.607 kb on - strand, within Atu4311 at 1437.640 kb on - strand at 1437.645 kb on + strand at 1437.653 kb on - strand at 1437.653 kb on - strand at 1437.767 kb on + strand at 1437.788 kb on + strand at 1437.788 kb on - strand at 1437.819 kb on - strand at 1437.847 kb on + strand at 1437.858 kb on + strand at 1437.890 kb on - strand at 1437.890 kb on - strand at 1437.997 kb on + strand, within Atu4312 at 1438.038 kb on - strand, within Atu4312 at 1438.182 kb on - strand, within Atu4312 at 1438.234 kb on + strand, within Atu4312 at 1438.242 kb on - strand, within Atu4312 at 1438.242 kb on - strand, within Atu4312
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,435,060 + Atu4309 0.16 +5.7 1,435,068 - Atu4309 0.17 +0.1 1,435,081 - Atu4309 0.18 +0.1 1,435,110 + Atu4309 0.21 +0.1 1,435,169 - Atu4309 0.27 +1.1 1,435,171 + Atu4309 0.27 +0.3 1,435,185 - Atu4309 0.28 -0.2 1,435,264 + Atu4309 0.36 +0.3 1,435,283 + Atu4309 0.38 +0.1 1,435,432 - Atu4309 0.52 +1.2 1,435,474 - Atu4309 0.56 +0.4 1,435,474 - Atu4309 0.56 -0.1 1,435,543 + Atu4309 0.63 -0.6 1,435,649 + Atu4309 0.73 -1.1 1,435,693 + Atu4309 0.78 -1.4 1,435,772 - Atu4309 0.85 -0.1 1,435,814 + Atu4309 0.89 +0.3 1,435,826 + -0.2 1,435,826 + -0.0 1,436,009 + +0.2 1,436,334 + Atu4310 0.25 -0.5 1,436,437 + Atu4310 0.33 -0.2 1,436,448 + Atu4310 0.34 -0.2 1,436,498 + Atu4310 0.38 +1.8 1,436,498 + Atu4310 0.38 +0.3 1,436,498 + Atu4310 0.38 +0.0 1,436,506 - Atu4310 0.38 +0.8 1,436,506 - Atu4310 0.38 -0.7 1,436,506 - Atu4310 0.38 +0.1 1,436,529 - Atu4310 0.40 +2.4 1,436,531 + Atu4310 0.40 -1.0 1,436,539 - Atu4310 0.41 -0.2 1,436,539 - Atu4310 0.41 +0.3 1,436,584 + Atu4310 0.45 -0.2 1,436,593 - Atu4310 0.45 +0.9 1,436,630 + Atu4310 0.48 -0.9 1,436,638 - Atu4310 0.49 -0.9 1,436,667 - Atu4310 0.51 +0.8 1,436,814 + Atu4310 0.63 +0.1 1,436,864 + Atu4310 0.67 -0.1 1,436,921 + Atu4310 0.72 +1.5 1,436,921 + Atu4310 0.72 -0.3 1,436,921 + Atu4310 0.72 -0.1 1,436,921 + Atu4310 0.72 +1.1 1,436,921 + Atu4310 0.72 +0.3 1,436,929 - Atu4310 0.72 +1.2 1,436,931 - Atu4310 0.72 +0.5 1,436,939 - Atu4310 0.73 +0.8 1,437,033 - Atu4310 0.80 -0.5 1,437,049 + Atu4310 0.82 +1.5 1,437,070 - Atu4310 0.83 -0.0 1,437,082 + Atu4310 0.84 +0.8 1,437,152 + -1.4 1,437,152 + -0.1 1,437,152 + -0.4 1,437,160 - +0.3 1,437,160 - +0.2 1,437,160 - -0.5 1,437,160 - +0.1 1,437,160 - -0.2 1,437,166 - -0.0 1,437,213 + -0.0 1,437,412 + Atu4311 0.21 +0.2 1,437,429 + Atu4311 0.26 -0.5 1,437,434 + Atu4311 0.28 +0.6 1,437,458 - Atu4311 0.36 +0.2 1,437,475 - Atu4311 0.41 +1.7 1,437,505 - Atu4311 0.50 -0.0 1,437,529 - Atu4311 0.58 +1.3 1,437,552 - Atu4311 0.65 +0.2 1,437,607 - Atu4311 0.82 +0.7 1,437,640 - -0.0 1,437,645 + -0.4 1,437,653 - -0.5 1,437,653 - -0.8 1,437,767 + -0.2 1,437,788 + -0.5 1,437,788 - +0.9 1,437,819 - +0.8 1,437,847 + +0.0 1,437,858 + +0.9 1,437,890 - -0.4 1,437,890 - -0.2 1,437,997 + Atu4312 0.11 +0.2 1,438,038 - Atu4312 0.13 -0.0 1,438,182 - Atu4312 0.17 -1.4 1,438,234 + Atu4312 0.19 -0.4 1,438,242 - Atu4312 0.19 +0.0 1,438,242 - Atu4312 0.19 -0.4
Or see this region's nucleotide sequence