Strain Fitness in Escherichia coli BW25113 around b2906

Experiment: LB Aerobic with Novobiocin 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgcvT and visC are separated by 447 nucleotidesvisC and ubiH are separated by 22 nucleotides b2905: gcvT - glycine cleavage system aminomethyltransferase T (NCBI), at 3,047,595 to 3,048,689 gcvT b2906: visC - hypothetical protein (NCBI), at 3,049,137 to 3,050,339 visC b2907: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase (NCBI), at 3,050,362 to 3,051,540 ubiH Position (kb) 3049 3050 3051Strain fitness (log2 ratio) -1 0 1 2 3at 3048.181 kb on - strand, within gcvTat 3048.181 kb on - strand, within gcvTat 3048.196 kb on - strand, within gcvTat 3048.196 kb on - strand, within gcvTat 3048.231 kb on - strand, within gcvTat 3048.231 kb on - strand, within gcvTat 3048.264 kb on - strand, within gcvTat 3048.306 kb on + strand, within gcvTat 3048.324 kb on - strand, within gcvTat 3048.339 kb on + strand, within gcvTat 3048.339 kb on + strand, within gcvTat 3048.399 kb on + strand, within gcvTat 3048.569 kb on + strand, within gcvTat 3048.569 kb on + strand, within gcvTat 3048.608 kb on - strandat 3048.624 kb on - strandat 3048.683 kb on + strandat 3048.683 kb on + strandat 3048.756 kb on + strandat 3048.756 kb on + strandat 3048.859 kb on - strandat 3048.859 kb on - strandat 3048.928 kb on - strandat 3049.195 kb on - strandat 3049.205 kb on - strandat 3049.205 kb on - strandat 3049.208 kb on - strandat 3049.208 kb on - strandat 3049.213 kb on - strandat 3049.310 kb on - strand, within visCat 3049.310 kb on - strand, within visCat 3049.315 kb on - strand, within visCat 3049.365 kb on + strand, within visCat 3049.406 kb on + strand, within visCat 3049.458 kb on + strand, within visCat 3049.474 kb on - strand, within visCat 3049.536 kb on + strand, within visCat 3049.544 kb on - strand, within visCat 3049.544 kb on - strand, within visCat 3049.546 kb on - strand, within visCat 3049.546 kb on - strand, within visCat 3049.546 kb on - strand, within visCat 3049.549 kb on + strand, within visCat 3049.563 kb on - strand, within visCat 3049.632 kb on + strand, within visCat 3049.947 kb on + strand, within visCat 3049.947 kb on + strand, within visCat 3049.999 kb on - strand, within visCat 3049.999 kb on - strand, within visCat 3050.041 kb on + strand, within visCat 3050.041 kb on + strand, within visCat 3050.041 kb on - strand, within visCat 3050.093 kb on + strand, within visCat 3050.093 kb on + strand, within visCat 3050.093 kb on + strand, within visCat 3050.093 kb on - strand, within visCat 3050.184 kb on + strand, within visCat 3050.241 kb on + strandat 3050.241 kb on + strandat 3050.370 kb on + strandat 3050.370 kb on + strandat 3050.376 kb on + strandat 3050.376 kb on + strandat 3050.399 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM
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3,048,181 - gcvT b2905 0.54 +0.1
3,048,181 - gcvT b2905 0.54 +1.0
3,048,196 - gcvT b2905 0.55 -0.2
3,048,196 - gcvT b2905 0.55 -0.2
3,048,231 - gcvT b2905 0.58 -0.1
3,048,231 - gcvT b2905 0.58 -0.7
3,048,264 - gcvT b2905 0.61 +1.0
3,048,306 + gcvT b2905 0.65 -0.3
3,048,324 - gcvT b2905 0.67 +0.9
3,048,339 + gcvT b2905 0.68 -0.1
3,048,339 + gcvT b2905 0.68 +0.2
3,048,399 + gcvT b2905 0.73 +0.2
3,048,569 + gcvT b2905 0.89 -1.7
3,048,569 + gcvT b2905 0.89 -0.4
3,048,608 - +0.7
3,048,624 - -0.3
3,048,683 + -1.2
3,048,683 + +1.5
3,048,756 + -0.2
3,048,756 + +0.8
3,048,859 - +2.2
3,048,859 - -1.1
3,048,928 - -1.5
3,049,195 - +2.4
3,049,205 - +1.1
3,049,205 - +1.3
3,049,208 - +0.1
3,049,208 - -0.7
3,049,213 - +2.8
3,049,310 - visC b2906 0.14 -0.3
3,049,310 - visC b2906 0.14 +0.0
3,049,315 - visC b2906 0.15 -0.7
3,049,365 + visC b2906 0.19 +3.3
3,049,406 + visC b2906 0.22 +1.6
3,049,458 + visC b2906 0.27 +2.4
3,049,474 - visC b2906 0.28 +2.1
3,049,536 + visC b2906 0.33 +2.7
3,049,544 - visC b2906 0.34 +1.6
3,049,544 - visC b2906 0.34 -0.1
3,049,546 - visC b2906 0.34 +2.3
3,049,546 - visC b2906 0.34 +2.0
3,049,546 - visC b2906 0.34 +2.0
3,049,549 + visC b2906 0.34 +2.3
3,049,563 - visC b2906 0.35 +1.7
3,049,632 + visC b2906 0.41 +2.8
3,049,947 + visC b2906 0.67 +2.0
3,049,947 + visC b2906 0.67 +2.8
3,049,999 - visC b2906 0.72 +1.5
3,049,999 - visC b2906 0.72 +0.3
3,050,041 + visC b2906 0.75 +2.4
3,050,041 + visC b2906 0.75 +2.5
3,050,041 - visC b2906 0.75 +2.4
3,050,093 + visC b2906 0.79 +1.5
3,050,093 + visC b2906 0.79 +2.3
3,050,093 + visC b2906 0.79 +0.8
3,050,093 - visC b2906 0.79 +2.2
3,050,184 + visC b2906 0.87 +1.6
3,050,241 + +1.8
3,050,241 + +1.6
3,050,370 + +0.7
3,050,370 + +1.0
3,050,376 + +1.0
3,050,376 + +0.6
3,050,399 - -0.4

Or see this region's nucleotide sequence