Strain Fitness in Escherichia coli BW25113 around b2492

Experiment: LB Aerobic with Novobiocin 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfR and focB are separated by 21 nucleotidesfocB and yfgO are separated by 37 nucleotides b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934 hyfR b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804 focB b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903 yfgO Position (kb) 2611 2612 2613Strain fitness (log2 ratio) -1 0 1 2at 2611.065 kb on + strand, within hyfRat 2611.090 kb on + strand, within hyfRat 2611.139 kb on - strand, within hyfRat 2611.139 kb on - strand, within hyfRat 2611.289 kb on + strand, within hyfRat 2611.392 kb on - strand, within hyfRat 2611.599 kb on - strand, within hyfRat 2611.599 kb on - strand, within hyfRat 2611.829 kb on - strandat 2611.950 kb on + strandat 2612.067 kb on - strand, within focBat 2612.067 kb on - strand, within focBat 2612.133 kb on - strand, within focBat 2612.145 kb on + strand, within focBat 2612.214 kb on + strand, within focBat 2612.214 kb on + strand, within focBat 2612.217 kb on - strand, within focBat 2612.217 kb on - strand, within focBat 2612.397 kb on + strand, within focBat 2612.425 kb on + strand, within focBat 2612.497 kb on - strand, within focBat 2612.579 kb on + strand, within focBat 2612.579 kb on + strand, within focBat 2612.589 kb on - strand, within focBat 2612.690 kb on - strand, within focBat 2612.690 kb on - strand, within focBat 2612.720 kb on - strandat 2612.720 kb on - strandat 2612.749 kb on + strandat 2612.749 kb on + strandat 2612.764 kb on + strandat 2612.764 kb on + strandat 2612.845 kb on - strandat 2612.845 kb on - strandat 2612.875 kb on - strandat 2612.875 kb on - strandat 2612.958 kb on - strand, within yfgOat 2612.986 kb on - strand, within yfgOat 2612.986 kb on - strand, within yfgOat 2613.179 kb on - strand, within yfgOat 2613.179 kb on - strand, within yfgOat 2613.183 kb on + strand, within yfgOat 2613.309 kb on - strand, within yfgOat 2613.313 kb on - strand, within yfgOat 2613.313 kb on - strand, within yfgOat 2613.320 kb on + strand, within yfgOat 2613.402 kb on + strand, within yfgOat 2613.402 kb on + strand, within yfgOat 2613.424 kb on - strand, within yfgOat 2613.629 kb on + strand, within yfgOat 2613.642 kb on - strand, within yfgOat 2613.642 kb on - strand, within yfgOat 2613.668 kb on - strand, within yfgOat 2613.668 kb on - strand, within yfgOat 2613.678 kb on + strand, within yfgOat 2613.750 kb on + strand, within yfgO

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM
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2,611,065 + hyfR b2491 0.57 -0.9
2,611,090 + hyfR b2491 0.58 +0.4
2,611,139 - hyfR b2491 0.60 +1.5
2,611,139 - hyfR b2491 0.60 -0.6
2,611,289 + hyfR b2491 0.68 +1.6
2,611,392 - hyfR b2491 0.73 -0.9
2,611,599 - hyfR b2491 0.83 +1.0
2,611,599 - hyfR b2491 0.83 -0.2
2,611,829 - +0.2
2,611,950 + -0.1
2,612,067 - focB b2492 0.13 -0.2
2,612,067 - focB b2492 0.13 +0.6
2,612,133 - focB b2492 0.21 -0.0
2,612,145 + focB b2492 0.22 -0.6
2,612,214 + focB b2492 0.30 +0.6
2,612,214 + focB b2492 0.30 +1.0
2,612,217 - focB b2492 0.31 -0.6
2,612,217 - focB b2492 0.31 +1.7
2,612,397 + focB b2492 0.52 +0.2
2,612,425 + focB b2492 0.55 +1.0
2,612,497 - focB b2492 0.64 +0.4
2,612,579 + focB b2492 0.73 +1.0
2,612,579 + focB b2492 0.73 -0.6
2,612,589 - focB b2492 0.75 -0.1
2,612,690 - focB b2492 0.86 +0.3
2,612,690 - focB b2492 0.86 -0.9
2,612,720 - -0.5
2,612,720 - -0.2
2,612,749 + +0.6
2,612,749 + -0.0
2,612,764 + -0.3
2,612,764 + -0.3
2,612,845 - +1.5
2,612,845 - -0.1
2,612,875 - +0.3
2,612,875 - -0.5
2,612,958 - yfgO b2493 0.11 +0.6
2,612,986 - yfgO b2493 0.14 -0.7
2,612,986 - yfgO b2493 0.14 -1.2
2,613,179 - yfgO b2493 0.32 +1.0
2,613,179 - yfgO b2493 0.32 -0.4
2,613,183 + yfgO b2493 0.32 +2.0
2,613,309 - yfgO b2493 0.44 +1.8
2,613,313 - yfgO b2493 0.44 -0.8
2,613,313 - yfgO b2493 0.44 -0.3
2,613,320 + yfgO b2493 0.45 +0.6
2,613,402 + yfgO b2493 0.53 -0.1
2,613,402 + yfgO b2493 0.53 +1.0
2,613,424 - yfgO b2493 0.55 -1.1
2,613,629 + yfgO b2493 0.74 -0.2
2,613,642 - yfgO b2493 0.75 +1.3
2,613,642 - yfgO b2493 0.75 +0.2
2,613,668 - yfgO b2493 0.78 -0.2
2,613,668 - yfgO b2493 0.78 +2.6
2,613,678 + yfgO b2493 0.79 -0.3
2,613,750 + yfgO b2493 0.85 +0.3

Or see this region's nucleotide sequence