Experiment: LB Aerobic with Novobiocin 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutA and eutH overlap by 4 nucleotides eutH and eutG are separated by 216 nucleotides
b2451: eutA - reactivating factor for ethanolamine ammonia lyase (NCBI), at 2,563,503 to 2,564,906
eutA
b2452: eutH - predicted inner membrane protein (NCBI), at 2,564,903 to 2,566,129
eutH
b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533
eutG
Position (kb)
2564
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1 at 2563.913 kb on - strand, within eutA at 2563.949 kb on - strand, within eutA at 2564.001 kb on + strand, within eutA at 2564.037 kb on - strand, within eutA at 2564.285 kb on + strand, within eutA at 2564.377 kb on - strand, within eutA at 2564.443 kb on - strand, within eutA at 2564.443 kb on - strand, within eutA at 2564.498 kb on - strand, within eutA at 2564.498 kb on - strand, within eutA at 2564.577 kb on - strand, within eutA at 2564.599 kb on + strand, within eutA at 2564.626 kb on + strand, within eutA at 2564.636 kb on - strand, within eutA at 2564.636 kb on - strand, within eutA at 2564.741 kb on + strand, within eutA at 2564.743 kb on + strand, within eutA at 2564.753 kb on - strand, within eutA at 2564.865 kb on + strand at 2565.050 kb on + strand, within eutH at 2565.126 kb on + strand, within eutH at 2565.171 kb on - strand, within eutH at 2565.210 kb on - strand, within eutH at 2565.259 kb on - strand, within eutH at 2565.275 kb on + strand, within eutH at 2565.275 kb on + strand, within eutH at 2565.331 kb on + strand, within eutH at 2565.353 kb on - strand, within eutH at 2565.416 kb on + strand, within eutH at 2565.454 kb on - strand, within eutH at 2565.454 kb on - strand, within eutH at 2565.593 kb on + strand, within eutH at 2565.593 kb on + strand, within eutH at 2565.770 kb on - strand, within eutH at 2565.772 kb on - strand, within eutH at 2565.783 kb on - strand, within eutH at 2565.838 kb on - strand, within eutH at 2565.840 kb on + strand, within eutH at 2565.931 kb on + strand, within eutH at 2565.948 kb on + strand, within eutH at 2565.981 kb on + strand, within eutH at 2565.981 kb on + strand, within eutH at 2566.315 kb on + strand at 2566.315 kb on + strand at 2566.364 kb on + strand at 2566.409 kb on - strand at 2566.431 kb on + strand at 2566.451 kb on - strand at 2566.515 kb on + strand, within eutG at 2566.522 kb on + strand, within eutG at 2566.522 kb on + strand, within eutG at 2566.585 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.637 kb on + strand, within eutG at 2566.894 kb on + strand, within eutG at 2566.914 kb on + strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2567.008 kb on + strand, within eutG at 2567.012 kb on - strand, within eutG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM remove 2,563,913 - eutA b2451 0.29 +0.1 2,563,949 - eutA b2451 0.32 +0.4 2,564,001 + eutA b2451 0.35 +0.0 2,564,037 - eutA b2451 0.38 -0.8 2,564,285 + eutA b2451 0.56 +0.5 2,564,377 - eutA b2451 0.62 +0.1 2,564,443 - eutA b2451 0.67 -0.2 2,564,443 - eutA b2451 0.67 -0.3 2,564,498 - eutA b2451 0.71 -1.8 2,564,498 - eutA b2451 0.71 -0.9 2,564,577 - eutA b2451 0.76 -0.3 2,564,599 + eutA b2451 0.78 +0.2 2,564,626 + eutA b2451 0.80 -0.3 2,564,636 - eutA b2451 0.81 -1.0 2,564,636 - eutA b2451 0.81 +0.2 2,564,741 + eutA b2451 0.88 -1.8 2,564,743 + eutA b2451 0.88 -0.2 2,564,753 - eutA b2451 0.89 -0.8 2,564,865 + -1.4 2,565,050 + eutH b2452 0.12 -1.6 2,565,126 + eutH b2452 0.18 +0.8 2,565,171 - eutH b2452 0.22 +1.2 2,565,210 - eutH b2452 0.25 +0.6 2,565,259 - eutH b2452 0.29 +0.4 2,565,275 + eutH b2452 0.30 +0.4 2,565,275 + eutH b2452 0.30 +1.4 2,565,331 + eutH b2452 0.35 +0.6 2,565,353 - eutH b2452 0.37 +0.2 2,565,416 + eutH b2452 0.42 +0.2 2,565,454 - eutH b2452 0.45 -0.6 2,565,454 - eutH b2452 0.45 -0.4 2,565,593 + eutH b2452 0.56 -0.0 2,565,593 + eutH b2452 0.56 +0.6 2,565,770 - eutH b2452 0.71 +0.4 2,565,772 - eutH b2452 0.71 +0.4 2,565,783 - eutH b2452 0.72 +0.4 2,565,838 - eutH b2452 0.76 +0.4 2,565,840 + eutH b2452 0.76 +0.4 2,565,931 + eutH b2452 0.84 +0.8 2,565,948 + eutH b2452 0.85 +0.2 2,565,981 + eutH b2452 0.88 +0.7 2,565,981 + eutH b2452 0.88 -1.8 2,566,315 + -1.4 2,566,315 + +0.8 2,566,364 + -0.3 2,566,409 - -0.2 2,566,431 + -1.0 2,566,451 - -0.0 2,566,515 + eutG b2453 0.14 +0.6 2,566,522 + eutG b2453 0.15 +1.3 2,566,522 + eutG b2453 0.15 +0.2 2,566,585 + eutG b2453 0.20 -1.0 2,566,620 + eutG b2453 0.23 +0.5 2,566,620 + eutG b2453 0.23 +0.5 2,566,637 + eutG b2453 0.24 -0.1 2,566,894 + eutG b2453 0.46 -0.3 2,566,914 + eutG b2453 0.48 -1.2 2,566,938 - eutG b2453 0.50 -1.2 2,566,938 - eutG b2453 0.50 -0.6 2,566,938 - eutG b2453 0.50 -0.4 2,567,008 + eutG b2453 0.56 -0.1 2,567,012 - eutG b2453 0.56 -0.6
Or see this region's nucleotide sequence