Experiment: LB Aerobic with Novobiocin 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdH and ybdL are separated by 108 nucleotides ybdL and ybdM are separated by 0 nucleotides ybdM and ybdN overlap by 28 nucleotides
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
b0601: ybdM - hypothetical protein (NCBI), at 633,970 to 634,599
ybdM
b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792
ybdN
Position (kb) 632
633
634 Strain fitness (log2 ratio) -2
-1
0
1
2 at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH at 632.877 kb on - strand at 632.877 kb on - strand at 632.948 kb on + strand, within ybdL at 633.223 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on - strand, within ybdL at 633.320 kb on - strand, within ybdL at 633.322 kb on - strand, within ybdL at 633.327 kb on - strand, within ybdL at 633.351 kb on - strand, within ybdL at 633.418 kb on + strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.541 kb on - strand, within ybdL at 633.551 kb on - strand, within ybdL at 633.570 kb on - strand, within ybdL at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL at 633.717 kb on - strand, within ybdL at 633.825 kb on + strand, within ybdL at 633.853 kb on - strand, within ybdL at 633.970 kb on + strand at 634.194 kb on + strand, within ybdM at 634.206 kb on + strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.309 kb on + strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.337 kb on - strand, within ybdM at 634.464 kb on - strand, within ybdM at 634.477 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.593 kb on + strand at 634.672 kb on + strand at 634.687 kb on + strand at 634.720 kb on - strand, within ybdN at 634.963 kb on + strand, within ybdN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM remove 631,825 + ybdH b0599 0.20 -1.3 631,828 - ybdH b0599 0.20 -0.5 631,828 - ybdH b0599 0.20 +1.5 631,833 - ybdH b0599 0.20 -0.4 631,833 - ybdH b0599 0.20 -0.0 631,926 + ybdH b0599 0.29 +2.3 631,926 - ybdH b0599 0.29 +0.7 632,035 + ybdH b0599 0.39 +0.5 632,048 + ybdH b0599 0.40 -0.3 632,147 + ybdH b0599 0.49 +0.2 632,160 + ybdH b0599 0.50 +0.1 632,399 + ybdH b0599 0.72 -0.6 632,466 - ybdH b0599 0.78 -0.8 632,581 + ybdH b0599 0.89 -0.0 632,877 - +0.3 632,877 - +0.2 632,948 + ybdL b0600 0.12 -0.4 633,223 + ybdL b0600 0.36 -0.3 633,228 + ybdL b0600 0.36 -1.5 633,228 + ybdL b0600 0.36 +0.7 633,228 - ybdL b0600 0.36 -0.5 633,320 - ybdL b0600 0.44 +2.1 633,322 - ybdL b0600 0.44 +0.3 633,327 - ybdL b0600 0.45 +0.2 633,351 - ybdL b0600 0.47 +0.1 633,418 + ybdL b0600 0.52 +1.4 633,418 - ybdL b0600 0.52 +0.1 633,418 - ybdL b0600 0.52 +0.8 633,442 - ybdL b0600 0.55 +0.6 633,442 - ybdL b0600 0.55 -1.2 633,541 - ybdL b0600 0.63 -1.5 633,551 - ybdL b0600 0.64 +0.4 633,570 - ybdL b0600 0.66 +0.4 633,572 + ybdL b0600 0.66 +0.6 633,587 + ybdL b0600 0.67 +1.3 633,597 - ybdL b0600 0.68 +0.2 633,717 - ybdL b0600 0.78 -0.2 633,825 + ybdL b0600 0.88 +0.9 633,853 - ybdL b0600 0.90 -0.4 633,970 + -0.4 634,194 + ybdM b0601 0.36 +0.6 634,206 + ybdM b0601 0.37 +1.1 634,214 - ybdM b0601 0.39 +0.1 634,214 - ybdM b0601 0.39 +0.4 634,214 - ybdM b0601 0.39 +0.9 634,214 - ybdM b0601 0.39 -1.8 634,214 - ybdM b0601 0.39 -0.1 634,309 + ybdM b0601 0.54 +0.7 634,335 - ybdM b0601 0.58 -0.4 634,335 - ybdM b0601 0.58 +0.7 634,337 - ybdM b0601 0.58 -0.4 634,464 - ybdM b0601 0.78 -0.5 634,477 - ybdM b0601 0.80 -2.2 634,486 - ybdM b0601 0.82 -0.5 634,486 - ybdM b0601 0.82 -0.2 634,593 + -0.0 634,672 + +0.2 634,687 + +0.2 634,720 - ybdN b0602 0.12 -0.5 634,963 + ybdN b0602 0.32 -0.9
Or see this region's nucleotide sequence