Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0319 and Atu0320 are separated by 36 nucleotides Atu0320 and Atu0321 are separated by 143 nucleotides Atu0321 and Atu0322 are separated by 46 nucleotides
Atu0319: Atu0319 - ubiquinone biosynthesis protein, at 312,080 to 313,654
Atu0319
Atu0320: Atu0320 - ubiquinone/menaquinone biosynthesis methyltransferase, at 313,691 to 314,467
Atu0320
Atu0321: Atu0321 - formamidopyrimidine-DNA glycosylase, at 314,611 to 315,507
Atu0321
Atu0322: Atu0322 - enoyl CoA hydratase, at 315,554 to 316,315
Atu0322
Position (kb) 314
315
316 Strain fitness (log2 ratio) -2
-1
0
1
2 at 313.696 kb on - strand at 314.542 kb on - strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.567 kb on + strand at 314.570 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.575 kb on - strand at 314.637 kb on + strand at 314.637 kb on + strand at 314.669 kb on + strand at 314.669 kb on + strand at 314.677 kb on - strand at 314.677 kb on - strand at 314.749 kb on + strand, within Atu0321 at 314.935 kb on + strand, within Atu0321 at 314.949 kb on + strand, within Atu0321 at 314.967 kb on + strand, within Atu0321 at 314.989 kb on - strand, within Atu0321 at 314.996 kb on + strand, within Atu0321 at 315.019 kb on - strand, within Atu0321 at 315.047 kb on - strand, within Atu0321 at 315.181 kb on + strand, within Atu0321 at 315.213 kb on + strand, within Atu0321 at 315.281 kb on - strand, within Atu0321 at 315.347 kb on + strand, within Atu0321 at 315.347 kb on + strand, within Atu0321 at 315.347 kb on + strand, within Atu0321 at 315.347 kb on + strand, within Atu0321 at 315.355 kb on - strand, within Atu0321 at 315.355 kb on - strand, within Atu0321 at 315.355 kb on - strand, within Atu0321 at 315.420 kb on - strand at 315.430 kb on - strand at 315.525 kb on + strand at 315.527 kb on + strand at 315.655 kb on - strand, within Atu0322 at 315.810 kb on - strand, within Atu0322 at 315.815 kb on + strand, within Atu0322 at 315.820 kb on + strand, within Atu0322 at 316.003 kb on + strand, within Atu0322 at 316.012 kb on - strand, within Atu0322 at 316.182 kb on + strand, within Atu0322 at 316.182 kb on + strand, within Atu0322 at 316.220 kb on - strand, within Atu0322
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 313,696 - -0.0 314,542 - -0.2 314,567 + +1.2 314,567 + -0.2 314,567 + -0.1 314,567 + -0.8 314,567 + -0.4 314,567 + -0.1 314,567 + +0.9 314,567 + +0.7 314,567 + +0.7 314,567 + -0.2 314,567 + +1.3 314,567 + -1.4 314,567 + -0.4 314,567 + -0.4 314,570 - -0.5 314,575 - +1.8 314,575 - +0.3 314,575 - -0.3 314,575 - +1.2 314,575 - -0.1 314,575 - +0.1 314,575 - -1.2 314,575 - +1.5 314,575 - -0.9 314,575 - -2.1 314,575 - +2.4 314,575 - -0.2 314,575 - +0.8 314,575 - -0.1 314,575 - -2.1 314,637 + +1.8 314,637 + -0.1 314,669 + +0.5 314,669 + +1.5 314,677 - +0.0 314,677 - +0.1 314,749 + Atu0321 0.15 +1.7 314,935 + Atu0321 0.36 -1.7 314,949 + Atu0321 0.38 -0.2 314,967 + Atu0321 0.40 +0.9 314,989 - Atu0321 0.42 +0.1 314,996 + Atu0321 0.43 -0.3 315,019 - Atu0321 0.45 +2.2 315,047 - Atu0321 0.49 +0.4 315,181 + Atu0321 0.64 +0.1 315,213 + Atu0321 0.67 +1.2 315,281 - Atu0321 0.75 +0.5 315,347 + Atu0321 0.82 +0.5 315,347 + Atu0321 0.82 +0.9 315,347 + Atu0321 0.82 -0.3 315,347 + Atu0321 0.82 +0.2 315,355 - Atu0321 0.83 +0.4 315,355 - Atu0321 0.83 +0.1 315,355 - Atu0321 0.83 -0.8 315,420 - -2.6 315,430 - -0.8 315,525 + +1.1 315,527 + -0.5 315,655 - Atu0322 0.13 -0.5 315,810 - Atu0322 0.34 +0.9 315,815 + Atu0322 0.34 -2.3 315,820 + Atu0322 0.35 +1.5 316,003 + Atu0322 0.59 -0.7 316,012 - Atu0322 0.60 -1.4 316,182 + Atu0322 0.82 -1.3 316,182 + Atu0322 0.82 +0.3 316,220 - Atu0322 0.87 -1.1
Or see this region's nucleotide sequence