Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4096 and PP_t59 are separated by 158 nucleotides PP_t59 and PP_4097 are separated by 63 nucleotides PP_4097 and PP_4098 are separated by 33 nucleotides
PP_4096: PP_4096 - conserved exported protein of unknown function, at 4,631,661 to 4,632,395
_4096
PP_t59: PP_t59 - tRNA-Gly, at 4,632,554 to 4,632,629
_t59
PP_4097: PP_4097 - CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase, at 4,632,693 to 4,633,253
_4097
PP_4098: PP_4098 - UvrABC system protein C, at 4,633,287 to 4,635,110
_4098
Position (kb)
4632
4633 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4631.556 kb on + strand at 4631.602 kb on + strand at 4631.602 kb on + strand at 4631.602 kb on + strand at 4631.603 kb on - strand at 4631.603 kb on - strand at 4631.604 kb on - strand at 4631.847 kb on + strand, within PP_4096 at 4631.848 kb on - strand, within PP_4096 at 4631.916 kb on + strand, within PP_4096 at 4631.917 kb on - strand, within PP_4096 at 4632.087 kb on + strand, within PP_4096 at 4632.087 kb on + strand, within PP_4096 at 4632.091 kb on + strand, within PP_4096 at 4632.091 kb on + strand, within PP_4096 at 4632.091 kb on + strand, within PP_4096 at 4632.091 kb on + strand, within PP_4096 at 4632.092 kb on - strand, within PP_4096 at 4632.092 kb on - strand, within PP_4096 at 4632.092 kb on - strand, within PP_4096 at 4632.092 kb on - strand, within PP_4096 at 4632.092 kb on - strand, within PP_4096 at 4632.095 kb on + strand, within PP_4096 at 4632.095 kb on + strand, within PP_4096 at 4632.233 kb on + strand, within PP_4096 at 4632.233 kb on + strand, within PP_4096 at 4632.240 kb on - strand, within PP_4096 at 4632.240 kb on - strand, within PP_4096 at 4632.361 kb on + strand at 4632.383 kb on + strand at 4632.383 kb on + strand at 4632.383 kb on + strand at 4632.384 kb on - strand at 4632.384 kb on - strand at 4632.384 kb on - strand at 4632.394 kb on - strand at 4632.543 kb on - strand at 4632.543 kb on - strand at 4632.551 kb on - strand at 4633.289 kb on - strand at 4633.289 kb on - strand at 4633.289 kb on - strand at 4633.295 kb on + strand at 4633.310 kb on - strand at 4633.332 kb on - strand at 4633.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 4,631,556 + -0.0 4,631,602 + -1.0 4,631,602 + -0.3 4,631,602 + -1.2 4,631,603 - -0.4 4,631,603 - -0.1 4,631,604 - -0.5 4,631,847 + PP_4096 0.25 -0.0 4,631,848 - PP_4096 0.25 -0.6 4,631,916 + PP_4096 0.35 +0.6 4,631,917 - PP_4096 0.35 -0.3 4,632,087 + PP_4096 0.58 -0.9 4,632,087 + PP_4096 0.58 -0.4 4,632,091 + PP_4096 0.59 +1.0 4,632,091 + PP_4096 0.59 +1.0 4,632,091 + PP_4096 0.59 -1.8 4,632,091 + PP_4096 0.59 -0.5 4,632,092 - PP_4096 0.59 +0.1 4,632,092 - PP_4096 0.59 -0.4 4,632,092 - PP_4096 0.59 +1.1 4,632,092 - PP_4096 0.59 -2.0 4,632,092 - PP_4096 0.59 +1.4 4,632,095 + PP_4096 0.59 +0.0 4,632,095 + PP_4096 0.59 -0.6 4,632,233 + PP_4096 0.78 +0.1 4,632,233 + PP_4096 0.78 +0.4 4,632,240 - PP_4096 0.79 -1.0 4,632,240 - PP_4096 0.79 -0.1 4,632,361 + +0.6 4,632,383 + -0.7 4,632,383 + -0.1 4,632,383 + -0.3 4,632,384 - +0.2 4,632,384 - +0.2 4,632,384 - +0.1 4,632,394 - +0.3 4,632,543 - +2.6 4,632,543 - +1.5 4,632,551 - -1.3 4,633,289 - -0.5 4,633,289 - +2.6 4,633,289 - +1.6 4,633,295 + +0.7 4,633,310 - -0.2 4,633,332 - -0.7 4,633,332 - -2.4
Or see this region's nucleotide sequence