Strain Fitness in Pseudomonas putida KT2440 around PP_5032

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
5,733,196	+		PP_5031	0.45	-0.3
5,733,196	+		PP_5031	0.45	+0.8
5,733,229	+		PP_5031	0.47	-0.9
5,733,230	-		PP_5031	0.47	-0.3
5,733,543	+		PP_5031	0.69	-1.7
5,733,558	+		PP_5031	0.70	-1.2
5,733,558	+		PP_5031	0.70	+0.4
5,733,559	-		PP_5031	0.70	-0.3
5,733,559	-		PP_5031	0.70	-1.3
5,733,637	+		PP_5031	0.76	+0.2
5,733,637	+		PP_5031	0.76	-0.5
5,733,637	+		PP_5031	0.76	-0.7
5,733,638	-		PP_5031	0.76	+0.7
5,733,638	-		PP_5031	0.76	-0.3
5,733,638	-		PP_5031	0.76	+2.5
5,733,638	-		PP_5031	0.76	+0.3
5,733,638	-		PP_5031	0.76	+0.2
5,733,641	+		PP_5031	0.76	-0.7
5,733,642	-		PP_5031	0.76	-0.2
5,733,691	+		PP_5031	0.80	+0.4
5,733,691	+		PP_5031	0.80	-0.4
5,733,691	+		PP_5031	0.80	+0.2
5,733,730	+		PP_5031	0.83	-2.7
5,733,731	-		PP_5031	0.83	-0.8
5,733,742	+		PP_5031	0.83	-0.7
5,733,742	+		PP_5031	0.83	-0.2
5,733,832	+		PP_5031	0.90	+0.2
5,733,883	+				+0.8
5,734,076	+				-0.0
5,734,076	+				-1.5
5,734,077	-				+0.5
5,734,077	-				+0.6
5,734,175	+				+1.8
5,734,238	-				+1.4
5,734,238	-				+1.2
5,734,755	+		PP_5032	0.41	+0.4
5,734,755	+		PP_5032	0.41	+2.5
5,735,017	+		PP_5032	0.58	-0.1
5,735,018	-				+2.0
5,735,018	-		PP_5032	0.58	+1.5
5,735,018	-		PP_5032	0.58	+0.8
5,735,018	-		PP_5032	0.58	+2.5
5,735,018	-		PP_5032	0.58	+0.7
5,735,053	+		PP_5032	0.60	+0.4
5,735,170	+		PP_5032	0.68	+1.5
5,735,171	-		PP_5032	0.68	+1.2
5,735,207	+		PP_5032	0.70	-0.3
5,735,207	+		PP_5032	0.70	+0.5
5,735,207	+		PP_5032	0.70	+0.3
5,735,207	+		PP_5032	0.70	-0.2
5,735,208	-		PP_5032	0.70	+0.8
5,735,208	-		PP_5032	0.70	+1.5
5,735,208	-		PP_5032	0.70	+0.4
5,735,208	-		PP_5032	0.70	+1.0
5,735,208	-		PP_5032	0.70	-0.6
5,735,358	+		PP_5032	0.80	+1.0
5,735,511	-				+1.9
5,735,666	+				+0.2
5,735,666	+				-0.3
5,735,667	-				-1.8
5,735,667	-				-0.5
5,735,667	-				-0.1
5,735,707	+				+2.5
5,735,736	-				+0.5
5,735,736	-				+0.3
5,736,218	+		PP_5033	0.27	-1.9
5,736,218	+		PP_5033	0.27	+1.2
5,736,218	+		PP_5033	0.27	-0.5
5,736,218	+		PP_5033	0.27	-0.9
5,736,218	+		PP_5033	0.27	-2.1
5,736,218	+		PP_5033	0.27	-0.7
5,736,219	-		PP_5033	0.27	+0.4
5,736,219	-		PP_5033	0.27	-0.3
5,736,325	+		PP_5033	0.33	+0.7
5,736,325	+		PP_5033	0.33	-0.9
5,736,325	+		PP_5033	0.33	-2.4
5,736,326	-		PP_5033	0.33	-0.4
5,736,326	-		PP_5033	0.33	-1.2
5,736,326	-		PP_5033	0.33	-0.4
5,736,326	-		PP_5033	0.33	+0.2
5,736,401	-		PP_5033	0.38	+0.5
5,736,401	-		PP_5033	0.38	-0.5