Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4627 and PP_4628 are separated by 21 nucleotides PP_4628 and PP_4629 are separated by 115 nucleotides PP_4629 and PP_5702 are separated by 59 nucleotides PP_5702 and PP_4630 overlap by 16 nucleotides
PP_4627: PP_4627 - transcriptional regulator, LysR family, at 5,251,158 to 5,252,039
_4627
PP_4628: PP_4628 - conserved protein of unknown function, at 5,252,061 to 5,252,516
_4628
PP_4629: PP_4629 - Sensory box protein, at 5,252,632 to 5,253,060
_4629
PP_5702: PP_5702 - conserved protein of unknown function, at 5,253,120 to 5,253,737
_5702
PP_4630: PP_4630 - Transcriptional regulator, MerR family, at 5,253,722 to 5,254,633
_4630
Position (kb) 5252
5253
5254 Strain fitness (log2 ratio) -1
0
1
2 at 5251.719 kb on - strand, within PP_4627 at 5251.734 kb on + strand, within PP_4627 at 5251.735 kb on - strand, within PP_4627 at 5251.965 kb on - strand at 5252.215 kb on + strand, within PP_4628 at 5252.216 kb on - strand, within PP_4628 at 5252.216 kb on - strand, within PP_4628 at 5252.219 kb on + strand, within PP_4628 at 5252.219 kb on + strand, within PP_4628 at 5252.227 kb on + strand, within PP_4628 at 5252.348 kb on + strand, within PP_4628 at 5252.486 kb on - strand at 5252.502 kb on - strand at 5252.725 kb on + strand, within PP_4629 at 5252.725 kb on + strand, within PP_4629 at 5252.746 kb on + strand, within PP_4629 at 5252.758 kb on + strand, within PP_4629 at 5252.759 kb on - strand, within PP_4629 at 5252.887 kb on + strand, within PP_4629 at 5252.888 kb on - strand, within PP_4629 at 5252.962 kb on + strand, within PP_4629 at 5252.962 kb on + strand, within PP_4629 at 5252.963 kb on - strand, within PP_4629 at 5253.163 kb on + strand at 5253.241 kb on + strand, within PP_5702 at 5253.269 kb on + strand, within PP_5702 at 5253.334 kb on + strand, within PP_5702 at 5253.334 kb on + strand, within PP_5702 at 5253.335 kb on - strand, within PP_5702 at 5253.631 kb on - strand, within PP_5702 at 5253.678 kb on + strand at 5253.678 kb on + strand at 5253.679 kb on - strand at 5253.679 kb on - strand at 5253.728 kb on - strand at 5253.728 kb on - strand at 5253.798 kb on - strand at 5253.851 kb on + strand, within PP_4630
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. remove 5,251,719 - PP_4627 0.64 -0.0 5,251,734 + PP_4627 0.65 -0.7 5,251,735 - PP_4627 0.65 +0.1 5,251,965 - -0.2 5,252,215 + PP_4628 0.34 -1.6 5,252,216 - PP_4628 0.34 -0.6 5,252,216 - PP_4628 0.34 -1.1 5,252,219 + PP_4628 0.35 -0.4 5,252,219 + PP_4628 0.35 -0.2 5,252,227 + PP_4628 0.36 -1.7 5,252,348 + PP_4628 0.63 -1.0 5,252,486 - -0.6 5,252,502 - -1.0 5,252,725 + PP_4629 0.22 -0.0 5,252,725 + PP_4629 0.22 +1.4 5,252,746 + PP_4629 0.27 +1.2 5,252,758 + PP_4629 0.29 +1.8 5,252,759 - PP_4629 0.30 -0.2 5,252,887 + PP_4629 0.59 -0.0 5,252,888 - PP_4629 0.60 +1.6 5,252,962 + PP_4629 0.77 +1.5 5,252,962 + PP_4629 0.77 +1.7 5,252,963 - PP_4629 0.77 +2.4 5,253,163 + +1.3 5,253,241 + PP_5702 0.20 -0.7 5,253,269 + PP_5702 0.24 -0.4 5,253,334 + PP_5702 0.35 +1.6 5,253,334 + PP_5702 0.35 -0.5 5,253,335 - PP_5702 0.35 +0.1 5,253,631 - PP_5702 0.83 +0.5 5,253,678 + +1.3 5,253,678 + -0.3 5,253,679 - -0.6 5,253,679 - +0.3 5,253,728 - -0.0 5,253,728 - +1.0 5,253,798 - -1.7 5,253,851 + PP_4630 0.14 -0.3
Or see this region's nucleotide sequence