Strain Fitness in Pseudomonas putida KT2440 around PP_4131

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
4,668,724	-		PP_4130	0.39	+2.0
4,668,724	-		PP_4130	0.39	+1.6
4,668,795	+		PP_4130	0.43	+2.6
4,668,795	+		PP_4130	0.43	+1.6
4,668,796	-		PP_4130	0.44	+2.6
4,668,917	+		PP_4130	0.51	+2.6
4,668,918	-		PP_4130	0.51	-0.3
4,669,032	+		PP_4130	0.59	+2.6
4,669,033	-		PP_4130	0.59	+1.6
4,669,125	+		PP_4130	0.65	+0.0
4,669,221	+		PP_4130	0.71	+2.6
4,669,387	+		PP_4130	0.82	+1.6
4,669,464	+		PP_4130	0.87	+0.6
4,669,465	-		PP_4130	0.87	+2.6
4,669,544	-				+1.6
4,669,567	-				+0.6
4,669,659	+				+0.6
4,669,895	-		PP_4131	0.15	+2.6
4,669,948	+		PP_4131	0.19	+2.6
4,669,948	+		PP_4131	0.19	+2.6
4,669,948	+		PP_4131	0.19	+0.6
4,669,996	+				-0.7
4,669,996	+		PP_4131	0.22	+2.6
4,669,996	+		PP_4131	0.22	+1.6
4,670,098	+		PP_4131	0.29	+2.6
4,670,113	+		PP_4131	0.30	+0.3
4,670,114	-		PP_4131	0.30	+1.0
4,670,156	-		PP_4131	0.33	+2.6
4,670,156	-		PP_4131	0.33	+2.6
4,670,212	+		PP_4131	0.37	+0.6
4,670,213	-		PP_4131	0.37	+3.2
4,670,371	+		PP_4131	0.48	+1.6
4,670,372	-		PP_4131	0.48	+2.6
4,670,429	+		PP_4131	0.52	+2.6
4,670,430	-		PP_4131	0.52	-0.2
4,670,430	-		PP_4131	0.52	+2.6
4,670,445	-		PP_4131	0.53	+0.3
4,670,495	+		PP_4131	0.56	+2.6
4,670,495	+		PP_4131	0.56	+3.6
4,670,496	-		PP_4131	0.56	+1.0
4,670,496	-		PP_4131	0.56	-0.5
4,670,496	-		PP_4131	0.56	+1.6
4,670,578	+		PP_4131	0.62	+2.6
4,670,579	-		PP_4131	0.62	-0.4
4,670,671	+		PP_4131	0.68	+2.6
4,670,671	+		PP_4131	0.68	+2.6
4,670,799	-		PP_4131	0.77	+1.6
4,670,863	+		PP_4131	0.81	+2.6
4,670,863	+		PP_4131	0.81	+2.6
4,670,864	-		PP_4131	0.81	+2.6
4,670,864	-		PP_4131	0.81	+2.6
4,670,941	+		PP_4131	0.87	+2.6
4,670,941	+		PP_4131	0.87	+2.6
4,670,941	+				+2.6
4,670,941	+		PP_4131	0.87	+2.6
4,670,945	-		PP_4131	0.87	+2.6
4,670,945	-		PP_4131	0.87	+0.6
4,670,948	-		PP_4131	0.87	+2.6
4,670,964	+		PP_4131	0.88	+1.0
4,671,210	-				-1.1
4,671,302	+		PP_4132	0.28	-0.1
4,671,303	-		PP_4132	0.28	+0.5
4,671,402	-		PP_4132	0.66	+0.6
4,671,438	+		PP_4132	0.79	-0.3
4,671,438	+		PP_4132	0.79	+1.0
4,671,439	-		PP_4132	0.80	+0.0
4,671,439	-		PP_4132	0.80	-1.1
4,671,442	+		PP_4132	0.81	-0.1
4,671,442	+		PP_4132	0.81	-1.2
4,671,442	+		PP_4132	0.81	-0.7
4,671,442	+		PP_4132	0.81	-0.4
4,671,442	+		PP_4132	0.81	-1.3
4,671,442	+		PP_4132	0.81	-0.5
4,671,443	-		PP_4132	0.81	+1.0
4,671,443	-		PP_4132	0.81	-0.0
4,671,443	-		PP_4132	0.81	-1.3
4,671,443	-		PP_4132	0.81	+0.6
4,671,443	-		PP_4132	0.81	+1.6
4,671,482	+				+0.3
4,671,482	+				-0.9
4,671,482	+				+1.6
4,671,482	+				+1.9
4,671,482	+				-2.0
4,671,483	-				+0.5
4,671,483	-				+0.1
4,671,483	-				+0.5
4,671,486	+				-0.8
4,671,487	-				-0.3
4,671,499	+				+1.6
4,671,499	+				+0.0
4,671,499	+				+0.2
4,671,506	+				+0.0
4,671,506	+				-0.7
4,671,506	+				+1.9
4,671,506	+				-1.0
4,671,506	+				-0.8
4,671,507	-				+1.4
4,671,507	-				+1.2
4,671,507	-				+3.6
4,671,507	-				+0.9
4,671,557	+				+0.3
4,671,558	-				-0.4
4,671,558	-				-0.6
4,671,558	-				-0.7
4,671,558	-				-0.8
4,671,649	+				+0.4
4,671,649	+				+0.6
4,671,649	+				-0.8
4,671,649	+				-0.2
4,671,649	+				+0.3
4,671,649	+				-0.3
4,671,649	+				+0.3
4,671,649	+				-1.0
4,671,649	+				-0.5
4,671,649	+				-0.6
4,671,649	+				+2.6
4,671,649	+				-0.9
4,671,649	+				+1.1
4,671,649	+				-0.3
4,671,664	+				+1.3
4,671,677	+				-1.2
4,671,823	+		PP_4133	0.40	-1.1
4,671,862	+		PP_4133	0.50	+0.2
4,672,093	+		PP_4134	0.13	-0.4