Strain Fitness in Pseudomonas putida KT2440 around PP_4120

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
4,655,685	+		PP_4118	0.83	+1.4
4,655,686	-		PP_4118	0.83	-0.2
4,655,762	-				+2.6
4,655,828	-				+1.0
4,655,946	+				-0.7
4,655,951	-				+1.6
4,655,966	-				-0.2
4,656,015	+				-0.1
4,656,021	+				-1.5
4,656,028	-				+1.6
4,656,062	+				-1.6
4,656,135	+				-0.2
4,656,169	+				+0.8
4,656,169	+				+0.3
4,656,172	-				+1.0
4,656,172	-				+1.0
4,656,181	+				+2.6
4,656,182	-				+1.6
4,656,384	-		PP_4119	0.48	+0.6
4,656,499	-				+2.6
4,656,524	+		PP_4119	0.82	+2.6
4,656,529	-		PP_4119	0.83	+2.6
4,656,529	-		PP_4119	0.83	+1.6
4,656,614	+				+2.6
4,656,614	+				+2.6
4,656,614	+				+2.6
4,656,615	-				+2.6
4,656,615	-				+1.0
4,656,615	-				+2.6
4,656,791	+		PP_4120	0.27	+2.6
4,656,815	+		PP_4120	0.31	+1.6
4,656,815	+		PP_4120	0.31	+2.6
4,656,962	+				+2.6
4,656,963	-		PP_4120	0.52	+1.6
4,657,029	-		PP_4120	0.62	+2.6
4,657,029	-		PP_4120	0.62	+2.0
4,657,029	-		PP_4120	0.62	+1.0
4,657,029	-		PP_4120	0.62	+2.6
4,657,082	+		PP_4120	0.70	+3.6
4,657,082	+		PP_4120	0.70	+2.6
4,657,082	+		PP_4120	0.70	+2.6
4,657,083	-		PP_4120	0.70	+0.3
4,657,083	-		PP_4120	0.70	+1.6
4,657,083	-		PP_4120	0.70	+2.6
4,657,083	-		PP_4120	0.70	+2.6
4,657,199	+		PP_4120	0.87	+2.6
4,657,200	-		PP_4120	0.87	+2.6
4,657,200	-		PP_4120	0.87	+0.6
4,657,254	+				+2.6
4,657,338	+				+2.6
4,657,338	+				-0.2
4,657,383	-				+0.3
4,657,383	-				+1.3
4,657,383	-				+2.6
4,657,398	+				+2.6
4,657,399	-				+1.6
4,657,399	-				+2.6
4,657,557	+		PP_4121	0.11	+2.6
4,657,558	-		PP_4121	0.11	+2.6
4,657,569	+		PP_4121	0.11	+1.0
4,657,644	+		PP_4121	0.16	+1.6
4,657,644	+		PP_4121	0.16	+2.6
4,657,644	+		PP_4121	0.16	+1.6
4,657,644	+		PP_4121	0.16	+2.6
4,657,644	+		PP_4121	0.16	+2.6
4,657,668	-		PP_4121	0.17	+2.6
4,657,756	-		PP_4121	0.22	+2.6
4,657,864	-				+2.6
4,657,864	-		PP_4121	0.28	+1.6
4,657,989	+		PP_4121	0.35	+0.6
4,657,989	+		PP_4121	0.35	+2.6
4,657,990	-		PP_4121	0.35	+1.0
4,657,990	-		PP_4121	0.35	+1.6
4,658,157	+				+2.6
4,658,158	-		PP_4121	0.45	+1.0
4,658,176	-		PP_4121	0.46	-0.8
4,658,205	+				+2.6