Strain Fitness in Pseudomonas putida KT2440 around PP_3746

Experiment: 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
4,274,557	+		PP_3745	0.34	+0.2
4,274,558	-		PP_3745	0.34	-0.6
4,274,558	-		PP_3745	0.34	-0.9
4,274,598	+		PP_3745	0.37	-1.0
4,274,599	-		PP_3745	0.37	-0.3
4,274,599	-		PP_3745	0.37	+0.4
4,274,753	+		PP_3745	0.47	-0.8
4,275,097	+		PP_3745	0.70	+0.6
4,275,097	+		PP_3745	0.70	-1.1
4,275,098	-		PP_3745	0.70	+1.9
4,275,098	-		PP_3745	0.70	+0.6
4,275,098	-		PP_3745	0.70	-1.2
4,275,098	-		PP_3745	0.70	+0.6
4,275,098	-		PP_3745	0.70	-0.3
4,275,098	-		PP_3745	0.70	+0.8
4,275,098	-		PP_3745	0.70	-0.1
4,275,098	-		PP_3745	0.70	-0.5
4,275,098	-		PP_3745	0.70	-1.3
4,275,132	-		PP_3745	0.73	+0.2
4,275,172	+		PP_3745	0.75	+0.2
4,275,173	-		PP_3745	0.75	-0.7
4,275,608	+				-0.1
4,275,609	-				+1.7
4,275,674	-		PP_3746	0.12	+0.3
4,275,708	+		PP_3746	0.16	-0.3
4,275,709	-		PP_3746	0.16	+0.9
4,275,709	-		PP_3746	0.16	+1.6
4,275,728	-		PP_3746	0.18	-0.3
4,275,905	-		PP_3746	0.34	+0.0
4,276,069	+		PP_3746	0.50	-0.7
4,276,182	+		PP_3746	0.61	+2.3
4,276,183	-		PP_3746	0.61	+0.2
4,276,471	-		PP_3746	0.88	+0.9
4,276,521	+				-0.6
4,276,619	+				+0.7
4,276,681	+				+1.0
4,276,681	+				-1.7
4,276,681	+				+0.6
4,276,682	-				-1.5
4,276,716	+				+0.8
4,276,716	+				+0.0
4,276,761	+		PP_3747	0.13	+3.0
4,276,762	-		PP_3747	0.13	-0.8
4,276,762	-		PP_3747	0.13	+1.1
4,276,875	+		PP_3747	0.22	+1.2
4,276,876	-		PP_3747	0.22	-1.7
4,277,033	-		PP_3747	0.35	-2.0
4,277,157	-		PP_3747	0.45	+0.5
4,277,292	-		PP_3747	0.56	+0.9
4,277,333	+		PP_3747	0.59	-0.6
4,277,376	+		PP_3747	0.62	-0.7
4,277,407	-		PP_3747	0.65	+0.2
4,277,407	-		PP_3747	0.65	-0.9